Laccases in Bioremediation and Waste Valorisation


Book Description

This Microbiology Monographs volume covers the latest advances in laccase applications in bioremediation and waste valorisation. The first three chapters provide a comprehensive introduction to fungal and bacterial laccases (the two most important enzyme groups from an application viewpoint) and their practical use in bioremediation and lignocellulosic waste valorisation. Subsequent chapters discuss possible combinations of laccases and further potentially collaborating enzymes, and offer in-depth insights into laccase immobilisation for wastewater treatment and environmental biosensor applications of laccases. Lastly, the book addresses the quest for enzymes with improved and better-fitting properties, covering laccase engineering by directed and computational evolution, and novel enzymes from extreme environments. As such, it is a fascinating read for microbiologists in both industry and academia.




Bacterial Laccases


Book Description

Bacterial Laccases: Engineering, Immobilization, Heterologous Production, and Industrial Applications provides a list of approaches that upgrades bacterial laccases to industrially relevant enzymes. Providing protocols for enzyme production and downstream processing, and including up-to-date information on bacterial laccases, as well as techniques that can be explored to enhance properties and production levels, this book clarifies research gaps and explains how they can be dealt with. Written for research scholars working on enzymes, this is a valuable tool for industries and policymakers. - Leads to a better understanding of traditional and novel methodologies for enhancing production and properties of bacterial laccases - Serves as a useful guide for researchers, industrialists and students - Includes chapters written by experts known for their contributions in respective areas - Describes the latest advances made in the field of bacterial laccases, including strategies that could be undertaken to improve their utility at an industrial level - Provides a comprehensive, up-to-date review of bacterial laccases and their important applications




Microbial Oxidative Enzymes


Book Description

Microbial oxidative enzymes are in need of today and in the future also. Several microbial oxidative enzymes are being used by various sectors like food, agriculture, medicine, detergents, leather, paper, etc. Microbial oxidative enzymes are a natural product, hence, the application of these enzymes is eco-friendly. Oxidative enzymes from microbes like bacteria, and fungi will be helpful in numerous applications including plant-soil health management, and waste treatments. This book will be more informative as well as useful for related industries and end users and will be of great value to those interested in present-day research on oxidation-reduction enzymes. In the coming years, this book will be a game changer for the field of oxidative enzyme development and its applications.




Microbial Enzymes


Book Description

Comprehensive discussion of production and purification strategies for microbial enzymes important to various industries, from food and beverages to pharmaceuticals Microbial Enzymes provides expert insight into diverse aspects of microbial enzymes, highlighting strategies for their production, purification, and manipulation, elucidating eco-friendly industrial applications, and discussing several production processes, such as the production of cellulose and non-synthetic indigo dye. This book emphasizes recent technological interventions in microbial enzyme technology like metagenomics, system biology, molecular biology, genomics, directed evolution, and bioinformatics. The important microbial enzymes highlighted in this book include xylanases, ureases, methane monooxygenase, polyhydroxyalkanoates, pectinases, peroxidases, α-L-rhamnosidase, alkane hydroxylases, laccases, proteases, gallic acid decarboxylase, chitinases, beta-glucosidase, lipases, inulinases, tannase, mycozyme, ACC deaminase, ligninolytic enzymes, and many more. Novel treatment methods involving strains of microorganisms with desirable properties applicable in the process of bioremediation through mitigating climate concern, increasing green production technology, improving agriculture productivity, and providing a means of earning a livelihood are discussed. Readers will also gain state-of-the-art background knowledge on existing technologies and their current challenges and future prospects. Contributed to by leading experts in the field and edited by four highly qualified academics, Microbial Enzymes explores important topics including: Strategies for the discovery and enhancement of enzyme function, and potentials of system biology to better understand the kinetics of industrially important enzymes Production and therapeutic applications of monoclonal antibodies in cancer and other diseases, and characterization of tannase as a virulence factor Opportunities to produce enzymes through food waste and byproducts, and recent developments in computational tools Use of Omics tools in the discovery of fungal enzymes and secondary metabolites Microbial Enzymes is a thorough and highly practical reference on the subject for students, scientists, biotechnologists, microbiologists, and policymakers working in environmental microbiology, biotechnology, and environmental sciences.




Laccase and Polyphenol Oxidase


Book Description

Laccase and Polyphenol Oxidase: Biochemistry and Biotechnological Applications, a new volume in the Foundations and Frontiers in Enzymology series, provides a thorough discussion of the laccase and polyphenol oxidase enzyme families, which play a key role in the oxidation of phenolic substrates and an important biological function in many organisms. In recent years, significant advances have been made in understanding these enzymes, but few practical resources exist on their fundamental biology, research methods, and applications. In this book, leading world experts discuss the biochemistry of laccase and polyphenol oxidases, research methods, and uses across industries, offering a practical overview of these important enzyme classes, and empowering researchers to develop their own studies and hone applications. The book deftly connects topics across these intricate enzyme systems, with early chapters on physicochemical properties of PPO and laccase, followed by separate sections on these related enzyme classes' function in biological and industrial systems, production, purification, active assays, and application areas from food and wine to healthcare, bioremediation, and green and computational chemistry. Step-by-step instructions and explanation offer clear guidance in replicating key research and application methodologies discussed. - Examines the fundamental biology of laccase and polyphenol oxidases, research methods, and their uses across industries - Features chapter contributions from leading international experts in the field - Offers step-by-step instructions in key research and application methodologies




Soil Bioremediation


Book Description

SOIL BIOREMEDIATION A practical guide to the environmentally sustainable bioremediation of soil Soil Bioremediation: An Approach Towards Sustainable Technology provides the first comprehensive discussion of sustainable and effective techniques for soil bioremediation involving microbes. Presenting established and updated research on emerging trends in bioremediation, this book provides contributions from both experimental and numerical researchers who provide reports on significant field trials. Soil Bioremediation instructs the reader on several different environmentally friendly bioremediation techniques, including: Bio-sorption Bio-augmentation Bio-stimulation Emphasizing molecular approaches and biosynthetic pathways of microbes, this one-of-a-kind reference focuses heavily on the role of microbes in the degradation and removal of xenobiotic substances from the environment and presents a unique management and conservation perspective in the field of environmental microbiology. Soil Bioremediation is perfect for undergraduate students in the fields of environmental science, microbiology, limnology, freshwater ecology and microbial biotechnology. It is also invaluable for researchers and scientists working in the areas of environmental science, environmental microbiology, and waste management.




Microorganisms for Sustainable Environment and Health


Book Description

Microorganisms for Sustainable Environment and Health covers hazardous pollutants released from natural as well as anthropogenic activities and implications on environmental and human health. This book serves as a valuable source of basic knowledge and recent developments in the clean technologies and pollution-associated diseases and abnormalities in the context of microorganisms. Focused on current solutions to various environmental problems in the field of bioremediation, it provides a detailed knowledge on the various types of toxic environmental pollutants discharged from different sources, their toxicological effects in environments, humans, animals and plants as well as their biodegradation and bioremediation approaches. This book helps environmental scientists and microbiologists learn about existing environmental problems and suggests ways to control or contain their effects by employing various treatment approaches. - Provides information on waste treatment approaches using microbes - Includes applications in biofuel and bioenergy production - Covers green belt development, hydroponics, phytoremediation, wetland treatment technology, and common effluent treatment plants (CETPs) - Discusses dissemination of antibiotic resistance among pathogenic microbes and strategies to combat multi-drug resistance (MDR)




Microbial Enzymes: Roles and Applications in Industries


Book Description

“Microbial Enzymes: Roles and applications in industry” offers an essential update on the field of microbial biotechnology, and presents the latest information on a range of microbial enzymes such as fructosyltransferase, laccases, amylases, lipase, and cholesterol oxidase, as well as their potential applications in various industries. Production and optimisation technologies for several industrially relevant microbial enzymes are also addressed. In recent years, genetic engineering has opened up new possibilities for redesigning microbial enzymes that are useful in multiple industries, an aspect that the book explores. In addition, it demonstrates how some of the emerging issues in the fields of agriculture, environment and human health can be resolved with the aid of green technologies based on microbial enzymes. The topics covered here will not only provide a better understanding of the commercial applications of microbial enzymes, but also outline futuristic approaches to use microbial enzymes as driver of industrial sustainability. Lastly, the book is intended to provide readers with an overview of recent applications of microbial enzymes in various industrial sectors, and to pique researchers’ interest in the development of novel microbial enzyme technologies to meet the changing needs of industry.




Microbes and Enzymes in Soil Health and Bioremediation


Book Description

Microbial enzymes play a vital role in maintaining soil health and removing pollutants from contaminated land. Soil microflora is closely associated with maintaining soil fertility, and the use of chemical pesticides, fertilizers and other volatile sprays in agriculture threatens the health ofthe microbial population in the soil. Every single particle of healthy soil contains millions of bacteria, which interact with the nutrients available, sustaining the nutrient cycle and making this microflora an essential component of life on earth. How do microbes help in the nutrient cycle? Either by intracellular digestion of macromolecules and converting these into smaller units in their metabolic pathways, or by secreting enzymes into the extracellular environment to facilitate the conversion of complex macromolecules into micro-molecules that can be easily absorbed by other living species. To meet demands for energy and food for the growing global population, it is important to protect agricultural land from contamination and maintain its productivity. Heavy metal ions from contaminated land canenter crops, fish or aquatic organismsvia contaminated water, and theseare then taken up by the human body, where they can accumulate and alter the normal microflora. The microbiological component of the soil is ahighly complex system and is still not fully understood. How do microbes survive in the changing physicochemical environment of soil?. This book helps readers understand the mechanism, various routes of microbialsoil remediation, the interactionsof different genera, and how microbial enzymes support the sustainable restoration of healthy soil.




Microbial Enzyme Technology in Food Applications


Book Description

The aim of food processing is to produce food that is palatable and tastes good, extend its shelf-life, increase the variety, and maintain the nutritional and healthcare quality of food. To achieve favorable processing conditions and for the safety of the food to be consumed, use of food grade microbial enzymes or microbes (being the natural biocatalysts) is imperative. This book discusses the uses of enzymes in conventional and non-conventional food and beverage processing as well as in dairy processing, brewing, bakery and wine making. Apart from conventional uses, the development of bioprocessing tools and techniques have significantly expanded the potential for extensive application of enzymes such as in production of bioactive peptides, oligosaccharides and lipids, flavor and colorants. Some of these developments include extended use of the biocatalysts (as immobilized/encapsulated enzymes), microbes (both natural and genetically modified) as sources for bulk enzymes, solid state fermentation technology for enzyme production. Extremophiles and marine microorganisms are another source of food grade enzymes. The book throws light on potential applications of microbial enzymes to expand the base of food processing industries.