Biosensors Based on Aptamers and Enzymes


Book Description

Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English.




Electrochemical Biosensors


Book Description

Electrochemical Biosensors summarizes fundamentals and trends in electrochemical biosensing. It introduces readers to the principles of transducing biological information to measurable electrical signals to identify and quantify organic and inorganic substances in samples. The complexity of devices related to biological matrices makes this challenging, but this measurement and analysis are critically valuable in biotechnology and medicine. Electrochemical biosensors combine the sensitivity of electroanalytical methods with the inherent bioselectivity of the biological component. Some of these sensor devices have reached the commercial stage and are routinely used in clinical, environmental, industrial and agricultural applications. - Describes several electrochemical methods used as detection techniques with biosensors - Discusses different modifiers, including nanomaterials, for preparing suitable pathways for immobilizing biomaterials at the sensor - Explains various types of signal monitoring, along with several recognition systems, including antibodies/antigens, DNA-based biosensors, aptamers (protein-based), and more




The Aptamer Handbook


Book Description

In The Aptamer Handbook, leading scientists from academia as well as biotech and pharma companies introduce the revolutionary concept of designing RNA and DNA oligonucleotides with novel functions by in vitro selection. These functions comprise high affinity binding (aptamers), catalytic activity (ribozymes and deoxyribozymes) or combinations of binding and catalytic properties (aptazymes). Basic concepts and technologies describing in detail how these functional oligonucleotides can be identified are presented. Numerous examples demonstrate the versatility of in vitro selected oligonucleotides. Special emphasis has been put on a section that shows the broad applicability of aptamers, e. g. in target validation, for analytics, or as new therapeutics. This first overview in the field is of prime interest for a broad audience of scientists both in academia and in industry who wish to expand their knowledge on the potential of new oligonucleotide functions and their applications.




Enzymes As Sensors


Book Description

Volume 589, the latest volume in the Methods in Enzymology series, focuses on enzymes as sensors. Contain contributions from leading authorities Informs and updates on all the latest developments in the field




Frontiers in Biosensorics I


Book Description

Volume I provides an in-depth discussion of the most recent developments of crucical biosensor components. It concentrates on the interface between the analyte phase and the detector, namely, the implementation of novel recognition elements, including nucleic acids, and of leading-edge technology in the construction of responsive thin layers. Thus, the reader can obtain a foretaste of achievable future progress in the field.




Ribozymes


Book Description

Ribozymes Provides comprehensive coverage of a core field in the molecular biosciences, bringing together decades of knowledge from the world’s top professionals in the field Timely and unique in its breadth of content, this all-encompassing and authoritative reference on ribozymes documents the great diversity of nucleic acid-based catalysis. It integrates the knowledge gained over the past 35 years in the field and features contributions from virtually every leading expert on the subject. Ribozymes is organized into six major parts. It starts by describing general principles and strategies of nucleic acid catalysis. It then introduces naturally occurring ribozymes and includes the search for new catalytic motifs or novel genomic locations of known motifs. Next, it covers the development and design of engineered ribozymes, before moving on to DNAzymes as a close relative of ribozymes. The next part examines the use of ribozymes for medicinal and environmental diagnostics, as well as for therapeutic tools. It finishes with a look at the tools and methods in ribozyme research, including the techniques and assays for structural and functional characterization of nucleic acid catalysts. The first reference to tie together all aspects of the multi-faceted field of ribozymes Features more than 30 comprehensive chapters in two volumes Covers the chemical principles of RNA catalysis; naturally occurring ribozymes, engineered ribozymes; DNAzymes; ribozymes as tools in diagnostics and therapy, and tools and methods to study ribozymes Includes first-hand accounts of concepts, techniques, and applications by a team of top international experts from leading academic institutions Dedicates half of its content to methods and practical applications, ranging from bioanalytical tools to medical diagnostics to therapeutics Ribozymes is an unmatched resource for all biochemists, biotechnologists, molecular biologists, and bioengineers interested in the topic.




Graphene Field-Effect Transistors


Book Description

Graphene Field-Effect Transistors In-depth resource on making and using graphene field effect transistors for point-of-care diagnostic devices Graphene Field-Effect Transistors focuses on the design, fabrication, characterization, and applications of graphene field effect transistors, summarizing the state-of-the-art in the field and putting forward new ideas regarding future research directions and potential applications. After a review of the unique electronic properties of graphene and the production of graphene and graphene oxide, the main part of the book is devoted to the fabrication of graphene field effect transistors and their sensing applications. Graphene Field-Effect Transistors includes information on: Electronic properties of graphene, production of graphene oxide and reduced graphene oxide, and graphene functionalization Fundamentals and fabrication of graphene field effect transistors, and nanomaterial/graphene nanostructure-based field-effect transistors Graphene field-effect transistors integrated with microfluidic platforms and flexible graphene field-effect transistors Graphene field-effect transistors for diagnostics applications, and DNA biosensors and immunosensors based on graphene field-effect transistors Graphene field-effect transistors for targeting cancer molecules, brain activity recording, bacterial detection, and detection of smell and taste Providing both fundamentals of the technology and an in-depth overview of using graphene field effect transistors for fabricating bioelectronic devices that can be applied for point-of-care diagnostics, Graphene Field-Effect Transistors is an essential reference for materials scientists, engineering scientists, laboratory medics, and biotechnologists.




Electrochemical DNA Biosensors


Book Description

This book focuses on the basic electrochemical applications of DNA in various areas, from basic principles to the most recent discoveries. The book comprises theoretical and experimental analysis of various properties of nucleic acids, research methods, and some promising applications. The topics discussed in the book include electrochemical detection of DNA hybridization based on latex/gold nanoparticle and nanotubes; nanomaterial-based electrochemical DNA detection; electrochemical detection of microorganism-based DNA biosensors; gold nanoparticle-based electrochemical DNA biosensors; electrochemical detection of the aptamer-target interaction; nanoparticle-induced catalysis for DNA biosensing; basic terms regarding electrochemical DNA (nucleic acids) biosensors; screen-printed electrodes for electrochemical DNA detection; application of field-effect transistors to label free electrical DNA biosensor arrays; and electrochemical detection of nucleic acids using branched DNA amplifiers.




Advanced Biosensors for Health Care Applications


Book Description

Advanced Biosensors for Health Care Applications highlights the different types of prognostic and diagnostic biomarkers associated with cancer, diabetes, Alzheimer's disease, brain and retinal diseases, cardiovascular diseases, bacterial infections, as well as various types of electrochemical biosensor techniques used for early detection of the potential biomarkers of these diseases. Many advanced nanomaterials have attracted intense interests with their unique optical and electrical properties, high stability, and good biocompatibility. Based on these properties, advanced nanoparticles have been used as biomolecular carriers, signal producers, and signal amplifiers in biosensor design. Recent studies reported that there are several diagnostic methods available, but the major issue is the sensitivity and selectivity of these approaches. This book outlines the need of novel strategies for developing new systems to retrieve health information of patients in real time. It explores the potential of nano-multidisciplinary science in the design and development of smart sensing technology using micro-nanoelectrodes, novel sensing materials, integration with MEMS, miniaturized transduction systems, novel sensing strategy, that is, FET, CMOS, System-on-a-Chip (SoC), Diagnostic-on-a-Chip (DoC), and Lab-on-a-Chip (LOC), for diagnostics and personalized health-care monitoring. It is a useful handbook for specialists in biotechnology and biochemical engineering. - Describes advanced nanomaterials for biosensor applications - Relates the properties of available nanomaterials to specific biomarkers applications - Includes diagnosis and electrochemical studies based on biosensors - Explores the potential of nano-multidisciplinary science to design and develop smart sensing technologies - Describes novel strategies for developing a new class of assay systems to retrieve the desired health information




Aptamers for Analytical Applications


Book Description

An essential guide that puts the focus on method developments and applications in aptamers In recent years, aptamer-based systems have been developed for a wide-range of analytical and medical applications. Aptamers for Analytical Applications offers an introduction to the topic, outlines the common protocols for aptamer synthesis, as well as providing information on the different optimization strategies that can obtain higher affinities to target molecules. The contributors?noted experts on the topic?provide an in-depth review of the characterization of aptamer-target molecule interaction and immobilization strategies and discuss the developments of methods for all the relevant applications. The book outlines different schemes to efficiently immobilize aptamers on substrates as well as summarizing the characterization methods for aptamer-ligand complexes. In addition, aptamer-based colorimetric, enzyme-linked, fluorescent, electrochemical, lateral flow and non-labeling analytical methods are presented. The book also reflects state-of-the-art and emerging applications of aptamer-based methods. This important resource: -Provides a guide to aptamers which provide highly specific and sensitive molecular recognition, with affinities in the range of antibodies and are much cheaper to produce -Offers a discussion of the analytical method developments and improvements with established systems and beyond -Offers a comprehensive guide to all the relevant application areas -Presents an authoritative book from contributors who are noted experts in the field Written for analytical chemists, biochemists, analytical researchers, Aptamers for Analytical Applications is a comprehensive book that adopts a methodological point of view to the important aspects of aptamer generation and modification with a strong emphasis on method developments for relevant applications.