Author : Martin E. Schimpf
Publisher : John Wiley & Sons
Page : 618 pages
File Size : 27,35 MB
Release : 2000-08-02
Category : Science
ISBN : 9780471184300
Field flow fractionation (FFF) is an emerging separation technique, which has been proven successful in the analysis of pharmaceuticals, biotechnology products, polymers, soils, and foods, among others. In this book, Martin Schimpf joins forces with Karin Caldwell and J. Calvin Giddings, two of the primary developers of this technique, to bring you the first comprehensive, one-stop reference on the technique.
Author : S. Kim R. Williams
Publisher : Springer Science & Business Media
Page : 306 pages
File Size : 34,25 MB
Release : 2011-12-07
Category : Science
ISBN : 3709101530
This is a timely collection of important biomedical applications for a set of separation/characterization techniques that are rapidly gaining popularity due to their wide dynamic range, high resolution, and ability to function in most commonly used solvent systems. Importantly, the field-flow fractionation (FFF) technique has recently emerged as a prominent complement to size exclusion chromatography for protein pharmaceuticals. Fractionation with FFF is gentle and preserves protein structural integrity better than existing alternatives. In the present text, different chapters are written by experts in their respective field of application, who offer comparisons between the FFF techniques and other methods for characterizing their special focus material. Practical guide-lines for successful implementation, such as choice of operating conditions, are offered in conjunction with each application. In addition to new instrumentation and approaches that address important current topics, readers are provided with an overall sense of prior (but timeless) major developments that may be overlooked in literature searches.
Author : Guilaume Greyling
Publisher : Springer
Page : 125 pages
File Size : 27,49 MB
Release : 2019-02-21
Category : Technology & Engineering
ISBN : 3030106500
This book introduces the reader to thermal field-flow fractionation and discusses its advantages over the fundamental problems associated with traditional column-based analytical techniques commonly used to characterize polymers and macromolecules. The authors discuss the theoretical background, equipment, experimental procedures as well as the recent advances and applications of thermal field-flow fractionation. Complete with several practical examples and troubleshooting guidelines, the book is written for beginners and experienced separation scientists alike and will enable its readers to optimize their experimental conditions for their specific separation needs and problems.
Author : Joseph Banoub
Publisher : Springer Science & Business Media
Page : 360 pages
File Size : 27,31 MB
Release : 2010-11-05
Category : Science
ISBN : 9048198798
The threat of biological and chemical terrorism has driven the demand for timely techniques that can quickly detect the agent or agents used in an attack. The detection and/or prevention of these potential security threats provide significant scientific and technical challenges due to the combination of possible agents and modes of delivery available. This book will present a thorough look at the importance and technological challenges of mass spectrometry (MS) for the detection & identification of biological and chemical threats. This new contribution’s general aims are to draw the attention of recognized practitioners, experts and graduate students trying to grasp the latest MS developments in the cutting-edge fields of MS-biodefense technologies for the rapid/early/specific sensitive threat detection of pathogens, viruses, explosives, mycotoxins, chemical agents, and biological markers of xenobiotic chemicals.
Author : Stepan Podzimek
Publisher : John Wiley & Sons
Page : 325 pages
File Size : 45,55 MB
Release : 2011-04-20
Category : Technology & Engineering
ISBN : 111810272X
A comprehensive, practical approach to three powerful methods of polymer analysis and characterization This book serves as a complete compendium of three important methods widely used for the characterization of synthetic and natural polymers—light scattering, size exclusion chromatography (SEC), and asymmetric flow field flow fractionation (A4F). Featuring numerous up-to-date examples of experimental results obtained by light scattering, SEC, and A4F measurements, Light Scattering, Size Exclusion Chromatography and Asymmetric Flow Field Flow Fractionation takes an all-in-one approach to deliver a complete and thorough explanation of the principles, theories, and instrumentation needed to characterize polymers from the viewpoint of their molar mass distribution, size, branching, and aggregation. This comprehensive resource: Is the only book gathering light scattering, size exclusion chromatography, and asymmetric flow field flow fractionation into a single text Systematically compares results of size exclusion chromatography with results of asymmetric flow field flow fractionation, and how these two methods complement each other Provides in-depth guidelines for reproducible and correct determination of molar mass and molecular size of polymers using SEC or A4F coupled with a multi-angle light scattering detector Offers a detailed overview of the methodology, detection, and characterization of polymer branching Light Scattering, Size Exclusion Chromatography and Asymmetric Flow Field Flow Fractionation should be of great interest to all those engaged in the polymer analysis and characterization in industrial and university research, as well as in manufacturing quality control laboratories. Both beginners and experienced can confidently rely on this volume to confirm their own understanding or to help interpret their results.
Author : Wim Jiskoot
Publisher : Springer Science & Business Media
Page : 694 pages
File Size : 24,7 MB
Release : 2005-12-05
Category : Medical
ISBN : 9780971176720
Protein pharmaceuticals form a fast-growing category in the arsenal of drugs. This book explores the nature of different analytical techniques and the way in which they are related to pharmaceutical proteins. In addition to serving the analytical chemist, this book is needed by the formulation scientist who is responsible for design and formulation of a pharmaceutical protein that can be monitored during production and over time.
Author : Jack Cazes
Publisher : CRC Press
Page : 2968 pages
File Size : 18,99 MB
Release : 2009-10-12
Category : Science
ISBN : 1439870675
Thoroughly revised and expanded, this third edition offers illustrative tables and figures to clarify technical points in the articles and provides a valuable, reader-friendly reference for all those who employ chromatographic methods for analysis of complex mixtures of substances. An authoritative source of information, this introductory guide to specific chromatographic techniques and theory discusses the relevant science and technology, offering key references for analyzing specific chemicals and applications in industry and focusing on emerging technologies and uses.
Author : Kevin J. Wilkinson
Publisher : John Wiley & Sons
Page : 702 pages
File Size : 28,84 MB
Release : 2007-01-30
Category : Science
ISBN : 047002433X
This text presents the current knowledge of environmental colloids and includes reviews of the current understanding of structure, role and behaviour of environmental colloids and particles, whilst focussing directly on aquatic systems and soils. In addition, there is substantial critical assessment of the techniques employed for the sampling, size fractionation and characterisation of colloids and particles. Chemical, physical and biological processes and interactions involving colloids are described, and particular attention is paid to quantitative approaches that take account of particle heterogeneity and polydispersity. Presents critical reviews of the state-of-the-art knowledge of environmental colloids Critical assessment of techniques employed for the sampling, size fractionation and characterisation of colloids and particles are given Theoretical and experimental aspects of the methods as well as the required developments and possible recommendations are discussed Each chapter gives a brief introduction general enough for the non-specialist Written by a internationally recognized group of contributors
Author :
Publisher : Elsevier
Page : 445 pages
File Size : 23,87 MB
Release : 2006-10-06
Category : Science
ISBN : 0080522548
The ability of polypeptides to form alternatively folded, polymeric structures such as amyloids and related aggregates is being increasingly recognized as a major new frontier in protein research. This new volume of Methods in Enzymology along with Part C (volume 413) on Amyloid, Prions and other Protein Aggregates continue in the tradition of the first volume (309) in containing detailed protocols and methodological insights, provided by leaders in the field, into the latest methods for investigating the structures, mechanisms of formation, and biological activities of this important class of protein assemblies. - Presents detailed protocols - Includes troubleshooting tips - Provides coverage on structural biology, computational methods, and biology
Author : Ann-Charlotte Eliasson
Publisher : CRC Press
Page : 537 pages
File Size : 20,80 MB
Release : 2017-08-23
Category : Technology & Engineering
ISBN : 1315355515
Carbohydrates in Food, Third Edition provides thorough and authoritative coverage of the chemical analysis, structure, functional properties, analytical methods, and nutritional relevance of monosaccharides, disaccharides, and polysaccharides used in food. Carbohydrates have become a hot topic in the debate about what to eat. This new edition includes increased treatment of resistant starch, dietary fiber, and starch digestion, especially in relation to different diets, suggesting that carbohydrate consumption should be reduced. New to the Third Edition: Explains how models for starch molecules have been improved recently leading to clearer understanding Discusses the growing interest in new sources of carbohydrates, such as chitosan and fructans, because of their function as prebiotics Features the latest developments on research into dietary fiber and starch digestion Carbohydrates in Food, Third Edition combines the latest data on the analytical, physicochemical, and nutritional properties of carbohydrates, offering a comprehensive and accessible single source of information. It evaluates the advantages and disadvantages of using various analytical methods, presents discussion of relevant physicochemical topics that relate to the use of carbohydrates in food that allow familiarity with important functional aspects of carbohydrates; and includes information on relevant nutritional topics in relation to the use of carbohydrates in food.
