Industrial Scale Suspension Culture of Living Cells


Book Description

The submersed cultivation of organisms in sterile containments or fermenters has become the standard manufacturing procedure, and will remain the gold standard for some time to come. This book thus addresses submersed cell culture and fermentation and its importance for the manufacturing industry. It goes beyond expression systems and integrally investigates all those factors relevant for manufacturing using suspension cultures. In so doing, the contributions cover all industrial cultivation methods in a comprehensive and comparative manner, with most of the authors coming from the industry itself. Depending on the maturity of the technology, the chapters address in turn the expression system, basic process design, key factors affecting process economics, plant and bioreactor design, and regulatory aspects.




Industrial Scale Suspension Culture of Living Cells


Book Description

The submersed cultivation of organisms in sterile containments or fermenters has become the standard manufacturing procedure, and will remain the gold standard for some time to come. This book thus addresses submersed cell culture and fermentation and its importance for the manufacturing industry. It goes beyond expression systems and integrally investigates all those factors relevant for manufacturing using suspension cultures. In so doing, the contributions cover all industrial cultivation methods in a comprehensive and comparative manner, with most of the authors coming from the industry itself. Depending on the maturity of the technology, the chapters address in turn the expression system, basic process design, key factors affecting process economics, plant and bioreactor design, and regulatory aspects.




Industrial Biotechnology


Book Description

The latest volume in the Advanced Biotechnology series provides an overview of the main product classes and platform chemicals produced by biotechnological processes today, with applications in the food, healthcare and fine chemical industries. Alongside the production of drugs and flavors as well as amino acids, bio-based monomers and polymers and biofuels, basic insights are also given as to the biotechnological processes yielding such products and how large-scale production may be enabled and improved. Of interest to biotechnologists, bio and chemical engineers, as well as those working in the biotechnological, chemical, and food industries.




Introduction to Cell and Tissue Culture


Book Description

It is a pleasure to contribute the foreword to Introduction to Cell and Tissue Culture: The ory and Techniques by Mather and Roberts. Despite the occasional appearance of thought ful works devoted to elementary or advanced cell culture methodology, a place remains for a comprehensive and definitive volume that can be used to advantage by both the novice and the expert in the field. In this book, Mather and Roberts present the relevant method ology within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology that renders technical cell culture information in a comprehensive, logical for mat. This allows topics to be presented with an emphasis on troubleshooting problems from a basis of understanding the underlying theory. The material is presented in a way that is adaptable to student use in formal courses; it also should be functional when used on a daily basis by professional cell culturists in a- demia and industry. The volume includes references to relevant Internet sites and other use ful sources of information. In addition to the fundamentals, attention is also given to mod ern applications and approaches to cell culture derivation, medium formulation, culture scale-up, and biotechnology, presented by scientists who are pioneers in these areas. With this volume, it should be possible to establish and maintain a cell culture laboratory devot ed to any of the many disciplines to which cell culture methodology is applicable.




Current Developments in Biotechnology and Bioengineering


Book Description

Current Developments in Biotechnology and Bioengineering: Bioprocesses, Bioreactors and Controls provides extensive coverage of new developments, state-of-the-art technologies, and potential future trends, reviewing industrial biotechnology and bioengineering practices that facilitate and enhance the transition of processes from lab to plant scale, which is becoming increasingly important as such transitions continue to grow in frequency. Focusing on industrial bioprocesses, bioreactors for bioprocesses, and controls for bioprocesses, this title reviews industrial practice to identify bottlenecks and propose solutions, highlighting that the optimal control of a bioprocess involves not only maximization of product yield, but also taking into account parameters such as quality assurance and environmental aspects. - Describes industrial bioprocesses based on the reaction media - Lists the type of bioreactors used for a specific bioprocess/application - Outlines the principles of control systems in various bioprocesses




Production of Biomass and Bioactive Compounds Using Bioreactor Technology


Book Description

The bioactive compounds of plants have world-wide applications in pharmaceutical, nutraceutical and food industry with a huge market. In this book, a group of active researchers have addressed on the most recent advances in plant cell and organ cultures for the production of biomass and bioactive compounds using bioreactors. Tremendous efforts have been made to commercialize the production of plant metabolites by employing plant cell and organ cultures in bioreactors. This book emphasizes on the fundamental topics like designing of bioreactors for plant cell and organ cultures, various types of bioreactors including stirred tank, airlift, photo-bioreactor, disposable bioreactor used for plant cell and organ cultures and the advantages and disadvantages of bioreactor cultures. Various strategies for biomass production and metabolite accumulation have been discussed in different plant systems including Korean/Chinese ginseng, Siberian ginseng, Indian ginseng, Echinacea, St. John’s wort, Noni, Chinese licorice, Caterpillar fungus and microalgae. Researches on the industrial application of plant cells and organs with future prospects as well as the biosafety of biomass produced in bioreactors are also described. The topics covered in this book, such as plant cell and organ cultures, hairy roots, bioreactors, bioprocess techniques, will be a valuable reference for plant biotechnologists, plant biologists, pharmacologists, pharmacists, food technologists, nutritionists, research investigators of healthcare industry, academia, faculty and students of biology and biomedical sciences. The multiple examples of large-scale applications of cell and organ cultures will be useful and significant to industrial transformation and real commercialization.




Plant Cell Culture Protocols


Book Description




Engineering Health


Book Description

Biotechnology harnesses cellular and biochemical systems to advance knowledge of the molecular cause of disease and to provide new diagnostic tools and more precisely targeted drugs. Within a decade, global investment in medical biotechnology has increased more than ten-fold, resulting in therapies for previously untreatable conditions. But what exactly is biotechnology and what are its origins? What further benefits to human health could it offer in the future? Written in an accessible style, contributors to this book explore the history behind different biotechnology tools, how they are used, and how they are reshaping the future of diagnostics, therapeutics and vaccines. Among the technologies examined are genetic engineering, DNA sequencing, monoclonal antibodies, stem cells, gene therapy, cancer immunotherapy and the most recent newcomer - synthetic biology. Applying new biotechnologies in medicine is not without great challenges. As medicines shift from small organic molecules to large, complex structures, such as therapeutic proteins, drugs become difficult to make, administer and regulate. This book will intrigue anyone interested in medicine and how we have been, and may continue to, engineer better health for ourselves. Such changes have major implications for how and where drugs are manufactured, the cost of medicine and the ethics of how far society is prepared to go to combat disease.




Biopharmaceuticals


Book Description

Biopharmaceuticals are derived from biological sources, either live organisms or their active components; nowadays, they are mainly produced by biotechnologies. Biopharmaceuticals are extensively used in the treatment of various diseases such as cardiovascular, metabolic, neurological diseases, cancer, and others for which there are no available therapeutic methods. With the advance of science, biopharmaceuticals have revolutionized the treatment, prevention, and diagnosis of many patients with disabling and life-threatening diseases. Innovative biopharmaceuticals definitely improve the life quality of patients and enhance the effectiveness of the healthcare system. This book encompasses the discovery, production, application, and regulation of biopharmaceuticals to demonstrate their research achievement, prospects, and challenges. We expect the significance of biopharmaceuticals to be revealed and emphasized by this book.




Green Biocatalysis


Book Description

Green Biocatalysis presents an exciting green technology that uses mild and safe processes with high regioselectivity and enantioselectivity. Bioprocesses are carried out under ambient temperature and atmospheric pressure in aqueous conditions that do not require any protection and deprotection steps to shorten the synthetic process, offering waste prevention and using renewable resources. Drawing on the knowledge of over 70 internationally renowned experts in the field of biotechnology, Green Biocatalysis discusses a variety of case studies with emphases on process R&D and scale-up of enzymatic processes to catalyze different types of reactions. Random and directed evolution under process conditions to generate novel highly stable and active enzymes is described at length. This book features: A comprehensive review of green bioprocesses and application of enzymes in preparation of key compounds for pharmaceutical, fine chemical, agrochemical, cosmetic, flavor, and fragrance industries using diverse enzymatic reactions Discussion of the development of efficient and stable novel biocatalysts under process conditions by random and directed evolution and their applications for the development of environmentally friendly, efficient, economical, and sustainable green processes to get desired products in high yields and enantiopurity The most recent technological advances in enzymatic and microbial transformations and cuttingedge topics such as directed evolution by gene shuffling and enzyme engineering to improve biocatalysts With over 3000 references and 800 figures, tables, equations, and drawings, Green Biocatalysis is an excellent resource for biochemists, organic chemists, medicinal chemists, chemical engineers, microbiologists, pharmaceutical chemists, and undergraduate and graduate students in the aforementioned disciplines.