Progress in Plant Protoplast Research


Book Description

Proceedings of the 7th International Protoplast Symposium, Wageningen, The Netherlands, December 6-11, 1987




Protoplast Fusion


Book Description

Although only about 3 years have passed since the preparation of the original manuscript of this book for the Russian edition, the number of successful experiments on somatic hybridization of higher plants has doubled. Although the main inferences of the first edition still remain in force, most of them have received conclusive experimental support and, moreover, some new con clusions have been drawn. It can be expected that these inferences and conclusions will constitute a more or less durable foundation for somatic cell genetics of higher plants. We thus hope this book will also remain useful over the next years, in spite of the rapid progress of experiments and the increase in the number of scientific reports in this field. Though it might appear strange to an uninvolved observer, the principal progress in hybridization of somatic cells of higher plants has been due to plant physiologists (who entered the field by elaborating methods and techniques for plant cell and, later, for isolated protoplast culture) rather than plant geneticists. However, further qualitative improvement in this field is inconceivable without the instillation of genetic ideology and the strict logic of genetic experiments. The main purpose ofthis book is the attempt to organize the available experimental data in terms and cate gories of genetic analysis. For this reason, this book lays no claim on being a comprehensive treatise on somatic hybridization.




Somaclonal Variation and Induced Mutations in Crop Improvement


Book Description

Genetic variability is an important parameter for plant breeders in any con ventional crop improvement programme. Very often the desired variation is un available in the right combination, or simply does not exist at all. However, plant breeders have successfully recombined the desired genes from cultivated crop gerrnplasm and related wild species by sexual hybridization, and have been able to develop new cultivars with desirable agronomie traits, such as high yield, disease, pest, and drought resistance. So far, conventional breeding methods have managed to feed the world's ever-growing population. Continued population growth, no further scope of expanding arable land, soil degradation, environ mental pollution and global warrning are causes of concern to plant biologists and planners. Plant breeders are under continuous pressure to improve and develop new cultivars for sustainable food production. However, it takes several years to develop a new cultivar. Therefore, they have to look for new technologies, which could be combined with conventional methods to create more genetic variability, and reduce the time in developing new cultivars, with early-maturity, and improved yield. The first report on induced mutation of a gene by HJ. Muller in 1927 was a major mi1estone in enhancing variation, and also indicated the potential applica tions of mutagenesis in plant improvement. Radiation sources, such as X-rays, gamma rays and fast neutrons, and chemical mutagens (e. g. , ethyl methane sulphonate) have been widely used to induce mutations.







Plant Cell, Tissue and Organ Culture


Book Description

This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operation and management as well as a glossary and all the information needed to set up and carry out any of the procedures without having to use other resource books. It is especially designed for professionals and advanced students who wish to acquire practical skills and first-hand experience in plant biotechnology.




Fungal Protoplasts


Book Description

Until now, information on fungal protoplasts has been scattered throughout various sources. With authoritative reviews of protoplast isolation and applications in fungal biology research, Fungal Protoplasts: Applications in Biochemistry and Genetics is the first volume devoted to a major area in experimental mycology-fungal protoplasts. Written by 18 pioneering experts, this unmatched, illustrated reference presents well-established knowledge of protoplast research as well as newer ideas and methods. The book encompasses advances in protoplast isolation techniques and methodology, uses of protoplasts in physiological, biochemical, and genetic studies, and developments in protoplast fusion that form the basis for transformation and gene cloning experiments, including applications in industrial biotechnology. This fact-filled book also features end-of-chapter bibliographies for further research.




Morphogenesis in Plant Tissue Cultures


Book Description

This book presents a detailed analysis of up-to-date literature on in vitro morphogenesis at cell, tissue, organ, and whole plant levels. Its driving force is the substantial advances made in the field of morphogenesis in tissue cultures during the last 25 years.




The Physiological Properties of Plant Protoplasts


Book Description

The idea for the p~esent book arose from a 3-day seminar which I organized in March 1984 for young research workers in plant physiology. Participants came from several universities of the French-speaking part of Switzerland and speakers from Basel, Mtinchen, Nottingham, Perpignan, Regensburg, Sheffield, Toulouse, Yale, ZUrich ... and Lausannne. The theme of the seminar was chosen from the range of research fields of our Institute. Meanwhile, feeling it was important to bear in mind that some of our hearers were not specialists in the chosen topic, I wished to pro vide a subject that would be of scientific, methodological and epistemologi cal interest. The critical analysis of the structural and functional characteristics of plant protoplasts exactly met these criteria. There exists ample material for discussion of the techniques of protoplast preparation, methods used in morphological, biological and biochemical studies, and for the comparison of protoplasts with the cells from which they are obtained.




Plant Genetic Resources of Legumes in the Mediterranean


Book Description

Genetic erosion, that is, the loss of native plant and genetic diversity has been exponential from the Mediterranean Basin through the Twentieth century. This careless eradication of species and genetic diversity as a result of human activities from a 'hot-spot' of diversity threatens sustainable agriculture and food security for the temperate regions of the world. Since the early 1900s there has been a largely ad hoc movement to halt the loss of plant diversity and enhance its utilisation. The Convention on Biological Diversity and Food and Agriculture Organisation of the United Nations International Undertaking on Plant Genetic Resources, both highlight the need to improve conservation methodologies and enhance utilisation techniques. It has been argued that the most important component of biodiversity is the genetic diversity of crop and forage species used to feed humans and livestock. These cultivated and related wild species provides the raw material for further selection and improvement. Leguminosae species are of major economic importance (peas, chickpeas, lentils and faba beans, as well as numerous forage species) and provide a particularly rich source of protein for human and animal foods. Their distribution is concentrated in the Mediterranean region and therefore the improvement of their conservation and use in the region is critical. This text is designed to help ensure an adequate breadth of legume diversity is conserved and to help maximise the use of that conserved diversity. The subjects of conservation and use of legume diversity, the Mediterranean ecosystem and taxonomy of legumes are introduced. Generic reviews of the taxonomy, centre of diversity, ecogeographic distribution, genetic diversity distribution, conservation status, conservation gaps and future research needs are provided, along with a discussion of the importance of rhizobia to the maintenance of legume diversity. Current ex situ and in situ conservation activities as well current legume uses are reviewed. In conclusion future priorities for ex situ and in situ plant genetic conservation and use of Mediterranean legumes are highlighted. All contributors look forward rather than simply reviewing past and current activities and therefore it is hoped that the identification of genetic erosion, location of taxonomic and genetic diversity and promotion of more efficient utilisation of conserved material will be enhanced.




In Vitro Haploid Production in Higher Plants


Book Description

Since the beginning of agricultural production, there has been a continuous effort to grow more and better quality food to feed ever increasing popula tions. Both improved cultural practices and improved crop plants have allowed us to divert more human resources to non-agricultural activities while still increasing agricultural production. Malthusian population predictions continue to alarm agricultural researchers, especially plant breeders, to seek new technologies that will continue to allow us to produce more and better food by fewer people on less land. Both improvement of existing cultivars and development of new high-yielding cultivars are common goals for breeders of all crops. In vitro haploid production is among the new technologies that show great promise toward the goal of increasing crop yields by making similar germplasm available for many crops that was used to implement one of the greatest plant breeding success stories of this century, i. e. , the development of hybrid maize by crosses of inbred lines. One of the main applications of anther culture has been to produce diploid homozygous pure lines in a single generation, thus saving many generations of backcrossing to reach homozygosity by traditional means or in crops where self-pollination is not possible. Because doubled haploids are equivalent to inbred lines, their value has been appreciated by plant breeders for decades. The search for natural haploids and methods to induce them has been ongoing since the beginning of the 20th century.