Histological & Histochemical Methods


Book Description

Revised and updated edition (1st was 1981) of a textbook on chemical and physical principles of fixation, staining and histochemistry. For students i all biological subjects using histological techniques, as well as researcher and medical laboratory technologists. Annotation copyright Book News, Inc




Phytoplasmas


Book Description




Basic Cell Culture Protocols


Book Description

At some point in their careers, virtually every scientist and technician, as well as many medical professionals, regardless of their area of specialization have a need to utilize cell culture systems. Updating and significantly expanding upon the previous editions, Basic Cell Culture Protocols, Fourth Edition provides the novice cell culturist with sufficient information to perform the basic techniques, to ensure the health and identity of their cell lines, and to be able to isolate and culture specialized primary cell types. The intent of this extensive volume is to generate a valuable resource containing clear methodologies pertinent to current areas of investigation, rather than attempting to educate cell culturists on specific cell types or organ systems. Written in the highly successful Methods in Molecular BiologyTM, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Basic Cell Culture Protocols, Fourth Edition compiles the essential techniques needed to approach this vital laboratory activity with full success.




New and Emerging Proteomic Techniques


Book Description

Leading researchers and innovators describe in step-by-step detail the latest techniques that promise to significantly impact the practice of proteomics, as well as its success in developing novel clinical agents. The methods span the entire spectrum of top-down and bottom-up approaches, including microarrays, gels, chromatography, and affinity separations, and address every aspect of the human proteome, both quantitatively and qualitatively. The techniques of protein detection utilized are diverse and range from fluorescence and resonance light scattering to surface plasmon resonance and mass spectrometry. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.




Molecular Methods in Plant Disease Diagnostics


Book Description

Using molecular methods for plant disease diagnosis provides diagnosticians with a number of advantages over more traditional methods. They can allow the identification of morphologically similar species, for example, or the detection of infection prior to symptom formation. Not only can molecular tools help by increasing the efficacy, accuracy and speed of diagnosis; their common technological basis provides further benefits, especially where resources are limited and traditional skills are hard to sustain. This book provides protocols for nucleic acid-based methods currently applied to plant pathogen detection and identification. It takes the practitioner through the full range of molecular diagnostic and detection methods and, as these generic techniques are appropriate for use on any target with minimal modification, also provides a useful resource for students of plant pathology and plant pathologists. Beginning with the background and future directions of the science, it then addresses DNA barcoding, microarrays, polymerase chain reactions (PCR), quality assurance and more, forming a complete reference on the subject.




Ion Channels


Book Description

The diverse applications in this volume range from the study of allosteric regulation of ion channel activity using a classic mutagenesis approach, to the study of channel subunit stoichiometry using a novel biophysical approach based on fluorescence resonance energy transfer. Highlights include methods for heterologous expression of ion channels in cells, for determining channel structure-function, and for studying channel regulation.




Histochemical Techniques


Book Description

Histochemical Techniques, 2nd Edition deals with different methods of tissue processing and their application to histochemical procedures, including freeze drying and freeze substitution. Topics covered include fixation, production of paraffin and frozen sections, and principles of enzyme histochemistry. The use of histochemical methods to demonstrate carbohydrates, proteins, amino acids, lipids, nucleic acids, pigments, and various enzymes is also discussed. This book is comprised of 20 chapters and begins with an overview of the problems and values of histochemistry. The discussion then turns to carbohydrates, along with concepts and methods in carbohydrate histochemistry such as blocking and enzyme methods. Other chapters focus on amyloids, pigments, minerals, phosphatases, and esterases. The principles of enzyme histochemistry are also considered, with emphasis on preservation and factors affecting enzyme activity. The final chapter is devoted to ultra-histochemistry, the application of histochemistry to electron microscopy. This monograph will be of interest to histochemists, histopathologists, and technologists involved in histochemical work.




Quantitative Marketing and Marketing Management


Book Description

Quantitative marketing has been gaining importance during the last decade. This is indicated by the growing number of model- and method-oriented studies published in leading journals as well as by the many successful applications of quantitative approaches in pricing, advertising, new product planning, and market segmentation decisions. In addition, market research has clearly benefitted from applying advanced quantitative models and methods in practice. Some 60 researchers – among them worldwide leading scholars – offer a broad overview of quantitative approaches in marketing. They not only highlight diverse mathematical and methodological perspectives, but also demonstrate the relevance and practical consequences of applying quantitative approaches to marketing problems.




Fundamentals of Quantum Computing


Book Description

This introductory book on quantum computing includes an emphasis on the development of algorithms. Appropriate for both university students as well as software developers interested in programming a quantum computer, this practical approach to modern quantum computing takes the reader through the required background and up to the latest developments. Beginning with introductory chapters on the required math and quantum mechanics, Fundamentals of Quantum Computing proceeds to describe four leading qubit modalities and explains the core principles of quantum computing in detail. Providing a step-by-step derivation of math and source code, some of the well-known quantum algorithms are explained in simple ways so the reader can try them either on IBM Q or Microsoft QDK. The book also includes a chapter on adiabatic quantum computing and modern concepts such as topological quantum computing and surface codes. Features: o Foundational chapters that build the necessary background on math and quantum mechanics. o Examples and illustrations throughout provide a practical approach to quantum programming with end-of-chapter exercises. o Detailed treatment on four leading qubit modalities -- trapped-ion, superconducting transmons, topological qubits, and quantum dots -- teaches how qubits work so that readers can understand how quantum computers work under the hood and devise efficient algorithms and error correction codes. Also introduces protected qubits - 0-π qubits, fluxon parity protected qubits, and charge-parity protected qubits. o Principles of quantum computing, such as quantum superposition principle, quantum entanglement, quantum teleportation, no-cloning theorem, quantum parallelism, and quantum interference are explained in detail. A dedicated chapter on quantum algorithm explores both oracle-based, and Quantum Fourier Transform-based algorithms in detail with step-by-step math and working code that runs on IBM QisKit and Microsoft QDK. Topics on EPR Paradox, Quantum Key Distribution protocols, Density Matrix formalism, and Stabilizer formalism are intriguing. While focusing on the universal gate model of quantum computing, this book also introduces adiabatic quantum computing and quantum annealing. This book includes a section on fault-tolerant quantum computing to make the discussions complete. The topics on Quantum Error Correction, Surface codes such as Toric code and Planar code, and protected qubits help explain how fault tolerance can be built at the system level.




Team Cohesion


Book Description

Research on Managing Groups and Teams provides a forum for truly novel ideas and emerging lines of inquiry across many group-related topics.