Book Description
Collection, isolation and maintenance of Rhizobia and Frankia; Colletion, cultivation and maintenance of azolla; Collection, isolation, cultivation and maintenance of associative N2-fixing bacteria; Fluorescence methods for study of Rhizobium in culture and in situ; Serological techniques for Brabyrhizobium and Rhizobium identification; Enzyme-linked immunosorbent assay (ELISA) for the detection and identification of Rhizobium strains; Use of Intrinsic culture and antibiotic resistance for Rhizobium Study; Isolation and identification of genetically marked strains of nitrogen-fixing microsymbionts of soybens; Measurement of nitrogen fixation by direct means; Measurement of biological fixation using acetylene reduction; Measuring ureides; Evaluation of nitrogen fixation by legumes in the greenhouse and growth chamber; Principles and practice of field designs to evaluate symbiotic nitrogen fixation; Production and quality control of inoculants; Role of culture collectios in biological nitrogen fixation.