RNA


Book Description

So much has been learned about RNA in the past ten years that the ability to purify, analyze, and manipulate RNA molecules is now essential in all kinds of bioscience. Initiating RNA research can be intimidating but the new book RNA: A Laboratory Manualprovides a broad range of up-to-date techniques presented in a functional framework, so that any investigator can confidently handle RNA and carry out meaningful experiments, from the most basic to the highly sophisticated. Originating in three of the field's most prominent laboratories, this manual provides the necessary background and strategies for approaching any RNA investigation, as well as detailed protocols and extensive tips and troubleshooting information. It is required reading for every research laboratory in the life sciences.




Antibodies


Book Description

Introduction to immunochemistry for molecular biologists and other nonspecialists. Spiral.




Live Cell Imaging


Book Description

Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book. The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.




A Laboratory Manual for Forensic Anthropology


Book Description

A Laboratory Manual for Forensic Anthropology approaches forensic anthropology as a modern and well-developed science, and includes consideration of forensic anthropology within the broader forensic science community, with extensive use of case studies and recent research, technology and challenges that are applied in field and lab contexts. This book covers all practical aspects of forensic anthropology, from field recoveries, to lab analyses, emphasizing hands-on activities. Topics include human osteology and odontology, examination methods, medicolegal significance, scene processing methods, forensic taphonomy, skeletal processing and sampling, sex estimation, ancestry estimation, age estimation, stature estimation, skeletal variation, trauma analysis, and personal identification. Although some aspects are specific to the United States, the vast majority of the material is internationally-relevant and therefore suitable for forensic anthropology courses in other countries. - Provides a comprehensive lab manual that is applicable to coursework in forensic anthropology and archaeology - Covers all practical aspects of forensic anthropology, from field recoveries, to lab analyses - Includes discussions of human osteology and odontology, examination methods, medicolegal significance, scene processing methods, forensic taphonomy, skeletal processing and sampling, sex estimation, and more - Emphasizes best practices in the field, providing an approach that is in line with today's professional forensic anthropology




Food Analysis Laboratory Manual


Book Description

This second edition laboratory manual was written to accompany Food Analysis, Fourth Edition, ISBN 978-1-4419-1477-4, by the same author. The 21 laboratory exercises in the manual cover 20 of the 32 chapters in the textbook. Many of the laboratory exercises have multiple sections to cover several methods of analysis for a particular food component of characteristic. Most of the laboratory exercises include the following: introduction, reading assignment, objective, principle of method, chemicals, reagents, precautions and waste disposal, supplies, equipment, procedure, data and calculations, questions, and references. This laboratory manual is ideal for the laboratory portion of undergraduate courses in food analysis.




Antibodies


Book Description

This second edition of the now-classic lab manual Antibodies, by Harlow and Lane, has been revised, extended, and updated by Edward Greenfield of the Dana-Farber Cancer Center, with contributions from other leaders in the field. Once again, the manual is an essential resource for molecular biology, immunology, and cell culture labs on all matters relating to antibodies. The chapters on hybridomas and monoclonal antibodies have been recast with extensive new information and there are additional chapters on characterizing antibodies, antibody engineering, and flow cytometry. As in the original book, the emphasis in this second edition is on providing clear and authoritative protocols with sufficient background information and troubleshooting advice for the novice as well as the experienced investigator.




Laboratory Manual for Introductory Geology


Book Description

Developed by three experts to coincide with geology lab kits, this laboratory manual provides a clear and cohesive introduction to the field of geology. Introductory Geology is designed to ease new students into the often complex topics of physical geology and the study of our planet and its makeup. This text introduces readers to the various uses of the scientific method in geological terms. Readers will encounter a comprehensive yet straightforward style and flow as they journey through this text. They will understand the various spheres of geology and begin to master geological outcomes which derive from a growing knowledge of the tools and subjects which this text covers in great detail.




Anatomy and Physiology, Laboratory Manual


Book Description

The Allen Laboratory Manual for Anatomy and Physiology, 6th Edition contains dynamic and applied activities and experiments that help students both visualize anatomical structures and understand complex physiological topics. Lab exercises are designed in a way that requires students to first apply information they learned and then critically evaluate it. With many different format options available, and powerful digital resources, it’s easy to customize this laboratory manual to best fit your course.




CRISPR-Cas


Book Description

CRISPR/Cas-based techniques are revolutionizing the way geneticists and molecular biologists modify DNA sequences and modulate gene expression in cells and organisms. This laboratory manual presents step-by-step protocols for applying this cutting-edge technology to any system of interest. Contributors describe approaches for de.




Imaging


Book Description

Imaging: A Laboratory Manual is organized into three sections, each containing background information and step-by-step protocols