Author : Verma, Ashish S./ Das Surajit & Singh Anchal
Publisher : S. Chand Publishing
Page : 760 pages
File Size : 48,81 MB
Release :
Category : Science
ISBN : 938374622X
Laboratory Manual in Biotechnology Students
Author : Robert N. Trigiano
Publisher : CRC Press
Page : 608 pages
File Size : 39,96 MB
Release : 2016-03-30
Category : Medical
ISBN : 1439896143
Under the vast umbrella of Plant Sciences resides a plethora of highly specialized fields. Botanists, agronomists, horticulturists, geneticists, and physiologists each employ a different approach to the study of plants and each for a different end goal. Yet all will find themselves in the laboratory engaging in what can broadly be termed biotechnol
Author : Oluf Gamborg
Publisher : Springer Science & Business Media
Page : 367 pages
File Size : 15,27 MB
Release : 2013-06-29
Category : Science
ISBN : 3642790488
This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operation and management as well as a glossary and all the information needed to set up and carry out any of the procedures without having to use other resource books. It is especially designed for professionals and advanced students who wish to acquire practical skills and first-hand experience in plant biotechnology.
Author : Heather B. Miller
Publisher : Academic Press
Page : 227 pages
File Size : 25,12 MB
Release : 2011-10-18
Category : Science
ISBN : 0123855454
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Author : H. S. Chawla
Publisher :
Page : 318 pages
File Size : 45,9 MB
Release : 2003
Category : Nature
ISBN :
Basics; Laboratory organization; Sterilization techniques; Nutrition medium; Choice of the explant; Plant tissue culture; Seed culture; Micropropagation- meristem culture; Micropropagation- axillary bud proliferation; Micropropagation- adventitious regeneration; Micropropagation- organogenesis; Micropropagation- embryogenesis; Cell suspension; Secondary metabolite production in a cell suspension culture; Anther culture; Protoplast isolation and fusion; Biotechnology; SDS-PAGE electrophoresis of proteins; Isolation of DNA from plant tissues; Isolation an purification of plasmid DNA; Restriction enzyme digestion of DNA; Agarose gel electrophoresis; Preparation of competent cells, transformation of E. coil with plasmid DNA and ligation of insert DNA to a vector; Agrobacterium-mediated gene transfer; Biolistic method of transformation in plants; In vitro amplification of DNA by PCR: detection of transgenes; RAPD analysis; Microsatellite marker analysis; Southerm biotting; Southerm hybridization.
Author : Chawla
Publisher : Oxford and IBH Publishing
Page : 318 pages
File Size : 41,60 MB
Release : 2004
Category : Plant biotechnology
ISBN : 9788120416130
This practical laboratory manual has been designed to familiarise students with protocols on plant tissue culture and recombinant DNA technology. It deals with the basic aspects on introduction, laboratory organization, sterilization techniques, nutrition medium and the choice of explant. It also has exercises on plant tissue culture: seed culture, embryo culture, meristem culture, node culture, axillary bud proliferation etc. A part of the manual also deals with recombinant DNA technology.
Author : P.T. Kalaichelvan
Publisher : MJP Publisher
Page : 267 pages
File Size : 28,74 MB
Release : 2019-06-11
Category : Medical
ISBN :
Safety Guidelines Microbial Cell Counting Microscopic Observation of Microorganisms Appendix-I Appendix-II
Author : Khalid Z. Masoodi
Publisher : Academic Press
Page : 220 pages
File Size : 44,12 MB
Release : 2020-10-28
Category : Science
ISBN : 012824450X
Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. - Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology - Features clear, step-by-step instruction for applying the techniques covered - Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment
Author : S. S. Purohit
Publisher :
Page : 408 pages
File Size : 23,43 MB
Release : 2004
Category : Plant biotechnology
ISBN : 9788177542226
Author : Carlos F. Barbas
Publisher : CSHL Press
Page : 724 pages
File Size : 43,54 MB
Release : 2001
Category : Science
ISBN : 0879697407
Phage-display technology has begun to make critical contributions to the study of molecular recognition. DNA sequences are cloned into phage, which then present on their surface the proteins encoded by the DNA. Individual phage are rescued through interaction of the displayed protein with a ligand, and the specific phage is amplified by infection of bacteria. Phage-display technology is powerful but challenging and the aim of this manual is to provide comprehensive instruction in its theoretical and applied so that any scientist with even modest molecular biology experience can effectively employ it. The manual reflects nearly a decade of experience with students of greatly varying technical expertise andexperience who attended a course on the technology at Cold Spring Harbor Laboratory. Phage-display technology is growing in importance and power. This manual is an unrivalled source of expertise in its execution and application.
