Author : Edward Harlow
Publisher : CSHL Press
Page : 748 pages
File Size : 41,73 MB
Release : 1988
Category : Medical
ISBN : 9780879693145
Introduction to immunochemistry for molecular biologists and other nonspecialists. Spiral.
Author : S. S. Purohit
Publisher :
Page : 408 pages
File Size : 21,4 MB
Release : 2004
Category : Plant biotechnology
ISBN : 9788177542226
Author : Robert N. Trigiano
Publisher : CRC Press
Page : 608 pages
File Size : 28,27 MB
Release : 2016-03-30
Category : Medical
ISBN : 1439896143
Under the vast umbrella of Plant Sciences resides a plethora of highly specialized fields. Botanists, agronomists, horticulturists, geneticists, and physiologists each employ a different approach to the study of plants and each for a different end goal. Yet all will find themselves in the laboratory engaging in what can broadly be termed biotechnol
Author : Oluf Gamborg
Publisher : Springer Science & Business Media
Page : 367 pages
File Size : 11,21 MB
Release : 2013-06-29
Category : Science
ISBN : 3642790488
This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operation and management as well as a glossary and all the information needed to set up and carry out any of the procedures without having to use other resource books. It is especially designed for professionals and advanced students who wish to acquire practical skills and first-hand experience in plant biotechnology.
Author : Heather B. Miller
Publisher : Academic Press
Page : 227 pages
File Size : 49,40 MB
Release : 2011-10-18
Category : Science
ISBN : 0123855454
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Author : M.S. Punia
Publisher : Scientific Publishers
Page : 263 pages
File Size : 38,74 MB
Release : 2018-01-01
Category : Technology & Engineering
ISBN : 9388172361
The book, “A Laboratory Manual of Plant Biotechnology and Molecular Biology” comprises of workable laboratory protocols for a large number of techniques related to plant biotechnology, genetic engineering and molecular biology. This includes plant cell and tissue culture, callus and suspension culture, anther culture, ovule culture, embryo culture, Cryopreservation, Isolation of Plant protoplasts, Protoplast culture and regeneration, production of somatic hybrids through protoplast fusion, gene transformation using Agrobacterium as vector, direct gene transfer using biolistic gun, Isolation of plant and organells DNA, construction and screening of genomic DNA libraries, Molecular markers like RFLP, RAPD, SCARS and CAPS, DNA sequencing, RNA isolation and northern blotting, Isolation of proteins and western blotting etc. The manual is prepared with the objective to cater the needs of post- graduate students as well as for scientists working in the disciplines of Plant Breeding, Genetics, Botany, Plant physiology, Biochemistry, Plant Biotechnology, Molecular Biology etc. It gives an update on some well established methods and presents reliable protocols.
Author : Robert D. Goldman
Publisher : CSHL Press
Page : 658 pages
File Size : 43,1 MB
Release : 2005
Category : Science
ISBN : 9780879696832
Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book. The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.
Author : C. Neal Stewart, Jr.
Publisher : John Wiley & Sons
Page : 554 pages
File Size : 12,48 MB
Release : 2012-12-13
Category : Science
ISBN : 1118589483
Designed to inform and inspire the next generation of plant biotechnologists Plant Biotechnology and Genetics explores contemporary techniques and applications of plant biotechnology, illustrating the tremendous potential this technology has to change our world by improving the food supply. As an introductory text, its focus is on basic science and processes. It guides students from plant biology and genetics to breeding to principles and applications of plant biotechnology. Next, the text examines the critical issues of patents and intellectual property and then tackles the many controversies and consumer concerns over transgenic plants. The final chapter of the book provides an expert forecast of the future of plant biotechnology. Each chapter has been written by one or more leading practitioners in the field and then carefully edited to ensure thoroughness and consistency. The chapters are organized so that each one progressively builds upon the previous chapters. Questions set forth in each chapter help students deepen their understanding and facilitate classroom discussions. Inspirational autobiographical essays, written by pioneers and eminent scientists in the field today, are interspersed throughout the text. Authors explain how they became involved in the field and offer a personal perspective on their contributions and the future of the field. The text's accompanying CD-ROM offers full-color figures that can be used in classroom presentations with other teaching aids available online. This text is recommended for junior- and senior-level courses in plant biotechnology or plant genetics and for courses devoted to special topics at both the undergraduate and graduate levels. It is also an ideal reference for practitioners.
Author : C. C. Giri
Publisher : I. K. International Pvt Ltd
Page : 157 pages
File Size : 16,22 MB
Release : 2013-12-30
Category : Plant biotechnology
ISBN : 8189866141
Plant Biotechnology: Practical Manual covers most of the important areas of present-day plant biotechnology, beginning from plant tissue culture media preparation to transgenic plant production and related molecular biology protocols. It is meant for both students who are being introduced to plant biotechnology and those wanting to do advance research in this field. It would also be helpful for teachers in formulating their own practical protocols using different model plant systems. This book includes the principles, theoretical background and the basis for each protocol supported by the authors own research findings. This approach has been adopted to help the learners and researchers modify their procedures to develop their own protocols and methods utilizing the proven protocols included in the book.
Author : Mark T. Windham
Publisher : CRC Press
Page : 432 pages
File Size : 17,84 MB
Release : 2003-07-28
Category : Science
ISBN : 9780849310379
Instructors, students and researchers in plant pathology have been searching for a primary text that combines an informal, easy-to-read style with a thorough introduction to the concepts and terminology of plant pathology. Plant Pathology Concepts and Laboratory Exercises answers their demand by presenting pathology principles, protocols and procedures, serving as a valuable resource tool for both students and researchers. This guide explains definitions of disease, characteristics of organisms that cause disease, and how diseases interact with hosts and the environment. Each topic is addressed by an expert in the field, and is supported by one or more lab exercises. The structure of the text allows for easy reading, with references minimized and major concepts highlighted at the beginning of each chapter. The laboratory exercises give added flexibility to instructors. There are experiments for both beginning and advanced students, and a broad choice of exercise topics that can be selected based upon the focus within each individual class. Step-by-step instructions are provided for each laboratory exercise.
