› › Advances In Immunohistochemistry

Immunohistochemistry and Immunocytochemistry

Author : Simon Renshaw

Publisher : John Wiley & Sons

Page : 258 pages

File Size : 20,63 MB

Release : 2017-02-06

Category : Science

ISBN : 1118717775

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Book Description

Immunohistochemistry and immunocytochemistry are invaluable tools for the visualization of tissue and cellular antigens in diagnostic and biological research environments. The need to obtain accurate, reliable and reproducible results is paramount. It is with this fundamental aim in mind that we have compiled Immunohistochemistry: Essential Methods. We have achieved this by examining each aspect of immunochemistry in turn, with each chapter including detailed information regarding the subject matter in question. Each chapter is written by an expert in their field and includes protocols that are typically used in their own research. Subjects covered are, amongst others, antibodies and their production; selection of reporter labels; immunochemical staining methods and experimental design (both using single and multiple reporter labels); quality assurance; automated immunochemistry; confocal microscopy and electron microscopy. In addition, benefits and limitations of each approach are discussed within the chapters.





Modern Immunohistochemistry

Author : Peiguo Chu

Publisher : Cambridge University Press

Page : 1179 pages

File Size : 14,85 MB

Release : 2014-02-27

Category : Medical

ISBN : 1316184455

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Book Description

Since the publication of the first edition of this best-selling book in 2009, the field of immunohistochemistry has advanced significantly. Fully updated to reflect the latest developments in the field, Modern Immunohistochemistry, Second Edition, is a practical guide to all the important diagnostic markers in each organ system. Concise text is supplemented by over 1,100 high-quality colour images and algorithms. The new edition features even more summary tables, highlighting the key points of differential immunophenotypic panels. A new, expanded introduction explains the basic principles of immunohistochemistry, and chapters have been updated to incorporate predictive/prognostic markers and the latest WHO classifications. All chapters are written by the same expert authors, providing a consistent, engaging style throughout and avoiding contradictory advice. An essential text for residents, this is also an extremely valuable resource for practitioners in anatomic pathology wishing to familiarise themselves with diagnostic markers at a quick glance.





Immunohistochemistry

Author : Trung Nguyen

Publisher : Cambridge University Press

Page : 283 pages

File Size : 25,35 MB

Release : 2022-07-07

Category : Medical

ISBN : 1009116053

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Book Description

This book will enable practitioners to understand the many complex intricacies of immunohistochemistry (IHC) and make best use of this powerful analytical tool. Providing a thorough grounding in the fundamentals of immunohistochemistry, the book includes several chapters on robotics and automation technology, giving key information on the design of machines and tips to maximise workflow efficiencies. The relationship between IHC and molecular pathology is explained clearly, demonstrating the increasing impact on personalized medicine and targeted therapies for cancer patients. The staining protocol is deconstructed, allowing the reader to adapt it for a variety of diagnostic and research applications. Written by experts at the forefront of hospital immunohistochemistry, there is a strong emphasis on practical guidance on a range of techniques as well as troubleshooting of common problems driven by the authors' experiences. Extensively illustrated with high-quality colour images, this is an invaluable resource to all pathology practitioners utilising the technique.



Biobanking

Author : William H. Yong

Publisher : Humana Press

Page : 470 pages

File Size : 27,77 MB

Release : 2018-12-14

Category : Medical

ISBN : 9781493989331

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Book Description

This volume not only discusses various common biobanking topics, it also delves into less-discussed subjects such as what is needed to start a biobank, training of new biobanking personnel, and ethnic representation in biospecimen research. Other chapters in this book span practical topics including: disaster prevention and recovery; information technology; flora and fauna preservation including zoological fluid specimen photography; surgical and autopsy biobanking; biobanking of bodily fluids; biosafety; cutting frozen sections; immunohistochemistry; nucleic acid extraction; and biospecimen shipping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Unique and comprehensive, Biobanking: Methods and Protocols is a valuable resource for novice and practicing biobankers, and for end-user researchers. This book aims to bring new insight into the field and expand on current biomedical biobanking studies.



Immunohistochemistry

Author : Charles Streckfus

Publisher : BoD – Books on Demand

Page : 142 pages

File Size : 20,63 MB

Release : 2020-03-04

Category : Medical

ISBN : 1838808205

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Book Description

Immunohistochemistry - The Ageless Biotechnology is a book that is ideal for undergraduate and graduate biomedical researchers, and medical and dental health professionals. It is a detailed text, which emphasizes the laboratory and clinical implications of immunohistochemistry. The text covers the advances of immunohistochemistry from its humble origins in the 1930s up to the new decade of 2020. The book also offers a review of the immunohistochemistry detection systems with emphasis on their principles, history, and their advantages. It also stipulates the limitations and delineates the factors that need to be considered for choosing an appropriate detection system for IHC applications. The book describes current laboratory techniques and new applications for the technology. As the reader will observe, the book provides new and useful information concerning the rapidly advancing field of immunohistochemistry.



Immunohistochemistry: Basics and Methods

Author : Igor B. Buchwalow

Publisher : Springer Science & Business Media

Page : 153 pages

File Size : 30,83 MB

Release : 2010-01-26

Category : Medical

ISBN : 3642046096

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Book Description

This concise yet comprehensive guide to the methods and protocols of immunohistochemistry covers established techniques and current developments in the field such as the use of epitope tags, multiple immunolabeling and diagnostic immunohistochemistry.



Immunohistochemistry in Diagnostic Dermatopathology

Author : Mai P. Hoang

Publisher : Cambridge University Press

Page : 315 pages

File Size : 30,54 MB

Release : 2017-09-28

Category : Medical

ISBN : 1107150167

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Book Description

One of only a few textbooks on the market dedicated to the important role of immunohistochemistry in diagnostic dermatopathology.



Antigen Retrieval Immunohistochemistry Based Research and Diagnostics

Author : Shan-Rong Shi

Publisher : Wiley

Page : 450 pages

File Size : 30,98 MB

Release : 2010-09-28

Category : Science

ISBN : 9780470624524

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Book Description

The most complete, up-to-date reference on antigen retrieval and immunohistochemistry An antigen is a substance that prompts the generation of antibodies and can cause an immune response. The antigen retrieval (AR) technique is in wide use across the globe, and is a critical technique used in medical diagnosis of disease, particularly clinical targeted cancer treatment. Antigen Retrieval Immunohistochemistry Based Research and Diagnostics discusses several scientific approaches to the standardization of quantifiable immunohistochemistry (IHC). Based on the development and application of AR by the editors, this volume summarizes recent achievements in AR-IHC and analyzes numerous cutting-edge issues for future research projects. Featuring contributions from a worldwide group of leading experts and research scientists in the field, this important work: Summarizes the key problems in the four fields of antigen retrieval Discusses the advances of AR techniques and their applications Provides practical methods and protocols in AR-IHC, such as extraction of nucleic acids and proteins for molecular analysis, cell/tissue sample preparation, and standardization and development of various techniques to meet the future needs of¿clinical and research molecular analysis Encourages further research in AR and IHC, particularly how AR methods might be employed for improved test performance and the development of greater reliability and reproducibility of IHC Includes an appendix of related laboratory protocols Antigen Retrieval Immunohistochemistry Based Research and Diagnostics is intended for clinical pathologists, molecular cell biologists, basic research scientists, technicians, and graduate students who undertake tissue/cell morphologic and molecular analysis and wish to use and extend the power of immunohistochemistry. It is also pertinent for most biotechnology companies majoring in development of IHC products. Wiley Series in Biomedical Engineering and Multi-Disciplinary Integrated Systems / Kai Chang, Series Editor