Author : Alison Campbell
Publisher : CRC Press
Page : 136 pages
File Size : 48,11 MB
Release : 2015-01-21
Category : Medical
ISBN : 1482214474
Unlike conventional single daily observations, time lapse technology provides hundreds of images, which allows pinpointing of key events in the embryo's in vitro development as well as the detection of brief but significant critical changes. This information is beneficial in selecting the most viable embryos from a cohort and increases the chances
Author : Alison Campbell
Publisher : CRC Press
Page : 644 pages
File Size : 41,64 MB
Release : 2024-03-22
Category : Medical
ISBN : 1003848915
Clinical scientists, embryologists, and reproductive technologists, at all levels, as well as trainees and students interested in assisted reproductive technology and reproductive medicine, will find here a clear synopsis of the best laboratory practice, clinical biology, assisted reproduction techniques, and advanced practical skills they will need to know as clinical practitioners. Expert embryologists and trainers contributed to the essential material as well as a number of advanced topics. Key features: Offers a clear synopsis of the clinical biology, laboratory skills, and best practice for the trainee embryologist Provides the ideal reference resource for those undertaking postgraduate training to become a clinical embryologist Gives access to the views of expert embryologist and trainers
Author : Catherine Racowsky
Publisher : Cambridge University Press
Page : 195 pages
File Size : 35,5 MB
Release : 2023-01-26
Category : Medical
ISBN : 1009035703
Selecting the best embryo to transfer to the uterus is key to successful in vitro fertilization (IVF). A huge amount of research has been devoted to this topic and there are numerous methods used, from simple morphological assessment to molecular biological techniques to assess the genome and metabolism of the newly fertilized embryo. For many of these techniques, an adequate evidence base is lacking, and expert opinion is valuable. Clinical imperatives require ranking all embryos in a cohort according to their viability, thereby enabling the selection of the best embryo to optimize live birth outcome: a key indicator used to measure and rate IVF Clinics worldwide. This clear and informative manual will provide embryologists and clinicians with an overview of the tools now available to assist in embryo selection, as well as evidence for their efficacy and safety and the broader considerations that must underlie these important clinical decisions.
Author : Kay Elder
Publisher : Cambridge University Press
Page : 399 pages
File Size : 37,16 MB
Release : 2020-03-12
Category : Medical
ISBN : 1108607411
This extensively updated new edition provides an indispensable account of modern in-vitro fertilization practice, building upon the popularity of previous editions. The authors initially give a comprehensive review of the biology of human gametes and embryos, before outlining basic to advanced IVF techniques. New developments in practical techniques and understanding are discussed, including in-vitro maturation, vitrification, preservation of fertility for cancer patients, stem cell technology, preimplantation genetic testing, and the role of epigenetics and imprinting. The revised introduction also incorporates a 'refresher' study review of fundamental principles of cell and molecular biology, now updated with current knowledge of meiosis in human oocytes, embryo metabolism and basic principles of genome editing. With high-quality illustrations and extensive, up-to-date reading lists, it is a must-have textbook for trainee and practising embryologists, as well as clinicians who are interested in the scientific principles that underpin successful IVF.
Author : Darren K. Griffin
Publisher : CRC Press
Page : 213 pages
File Size : 46,3 MB
Release : 2020-06-01
Category : Medical
ISBN : 0429820305
Preimplantation genetic testing (PGT) is now well established as a valuable treatment option for patients wishing to start or continue a family, for a range of indications from advanced maternal age to high risk of transmitting inherited disease. This text brings together contemporary thinking from international opinion leaders and will be an invaluable guide for practitioners in Reproductive Medicine wishing to keep pace with the latest developments and clinical data.
Author : Ruth Bellairs
Publisher : Elsevier
Page : 495 pages
File Size : 24,81 MB
Release : 2005-09-15
Category : Science
ISBN : 0080454755
This outstanding work is the only modern book devoted to the chick embryo and has been an essential resource for geneticists, molecular and developmental biologists, and other life scientists who use the chick embryo as their research model. This new enlarged and updated second edition is published in response to continuing demand. The text provides a detailed description of development, from fertilization to hatching, with emphasis on the earlier stages though also covering individual organ systems in detail. There are reviews of the more recent molecular research and a new section highlighting the important landmarks in the history of chick embryology which have had an impact on our understanding of developmental processes. The book is beautifully illustrated with 74 text-figures and over 500 photographs, including nearly 200 new scanning electron micrographs. - Updated and expanded text to accompany diagrams - More than 200 new labelled scanning electron micrographs showing individual tissues in great detail - Reviews of recent molecular research - Discusses the roles of genes such as Hox genes, BMPs, and sonic hedgehog during early development - New sections on genetical anomalies, techniques, and the poultry industry
Author :
Publisher : Academic Press
Page : 426 pages
File Size : 24,98 MB
Release : 2011-09-21
Category : Technology & Engineering
ISBN : 0080925294
This revised edition will continue to serve as the most complete and up-to-date guide to the use of the avian embryo in studies of vertebrate development. It will include new approaches to analysis of the chick genome, gene knock-out studies using RNA interference, morpholinos, and other cutting edge techniques. As with the original edition, emphasis has been placed on providing practical guidance, highlighting potentials and pitfalls of all key cell biological and embryological techniques. - Fully revised second edition - Organized into basic and advanced Methods - New section on Functional Genomics
Author : Kevin Coward
Publisher : Cambridge University Press
Page : 408 pages
File Size : 28,37 MB
Release : 2013-10-31
Category : Medical
ISBN : 110727625X
The success of Assisted Reproductive Technology is critically dependent upon the use of well optimized protocols, based upon sound scientific reasoning, empirical observations and evidence of clinical efficacy. Recently, the treatment of infertility has experienced a revolution, with the routine adoption of increasingly specialized molecular biological techniques and advanced methods for the manipulation of gametes and embryos. This textbook – inspired by the postgraduate degree program at the University of Oxford – guides students through the multidisciplinary syllabus essential to ART laboratory practice, from basic culture techniques and micromanipulation to laboratory management and quality assurance, and from endocrinology to molecular biology and research methods. Written for all levels of IVF practitioners, reproductive biologists and technologists involved in human reproductive science, it can be used as a reference manual for all IVF labs and as a textbook by undergraduates, advanced students, scientists and professionals involved in gamete, embryo or stem cell biology.
Author : Carlos Simón
Publisher : CRC Press
Page : 411 pages
File Size : 27,43 MB
Release : 2022-06-07
Category : Medical
ISBN : 1000567362
Different genetic diagnostic and treatment options are used worldwide to improve routine IVF procedures for the benefit of patients. This handbook updates the new genetic diagnostic technologies that have been translated to the clinic, aiming to improve outcomes in the clinic and result in a healthy baby in the home. Chapters cover the use of genetic technologies in a personalized manner to unravel the possible genetic risks for the couple wishing to conceive, in terms of sperm, the embryo, the endometrium, miscarriage, and finally the fetus. This expanded new edition covers the range of the latest genetic diagnostic technologies being translated into practice internationally to improve routine IVF procedures for the benefit of patients. Bringing together international experts to discuss their work, this text gives a context for the developments in this very fast-moving area of research and offers a comprehensive and rounded appraisal of hot topics.
Author : Patrick Quinn
Publisher : Cambridge University Press
Page : 307 pages
File Size : 18,79 MB
Release : 2014-03-27
Category : Medical
ISBN : 1139917358
This volume describes culture media and solutions used in human ART; how they have been developed for in vitro human pre-implantation embryo development, the function and importance of the various components in media and solutions and how they interact, and how the systems in which these are used can influence outcomes. Chapters discuss inorganic solutes, energy substrates, amino acids, macromolecules, cytokines, growth factors, buffers, pH, osmolality, and the interaction of these parameters. The role of incubators and other physical factors are reviewed, along with the relevance and prospects of emerging technologies: morphokinetic analysis using time-lapse imaging and dynamic fluid incubation systems. Results of prospective randomized trials are emphasized to ascertain the added value of these techniques for selecting viable embryos. This comprehensive guide will be invaluable for embryologists, physicians and all personnel involved in the fluid products used in human ART seeking to optimize their successful use of these components.
