Cytoskeleton Dynamics


Book Description

This volume details comprehensive state-of-the-art methods on actin microfilaments and microtubules and how they work to achieve different cellular functions in different cellular contexts. Chapters guide readers through protein purification, in vitro reconstitution of several cytoskeleton properties, analyses of microtubule- and actin-based structures, functional dissection of post-translational modifications, and roles in several biological processes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cytoskeleton Dynamics: Methods and Protocols aims to provide a wide range of experimental approaches and be an invaluable resource for present and future generations of cytoskeleton researchers. The chapter “Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.







Cytoskeleton


Book Description

The cytoskeleton is a highly dynamic intracellular platform constituted by a three-dimensional network of proteins responsible for key cellular roles as structure and shape, cell growth and development, and offering to the cell with "motility" that being the ability of the entire cell to move and for material to be moved within the cell in a regulated fashion (vesicle trafficking). The present edition of Cytoskeleton provides new insights into the structure-functional features, dynamics, and cytoskeleton's relationship to diseases. The authors' contribution in this book will be of substantial importance to a wide audience such as clinicians, researches, educators, and students interested in getting updated knowledge about molecular basis of cytoskeleton, such as regulation of cell vital processes by actin-binding proteins as cell morphogenesis, motility, their implications in cell signaling, as well as strategies for clinical trial and alternative therapies based in multitargeting molecules to tackle diseases, that is, cancer.




Mechanics of Motor Proteins and the Cytoskeleton


Book Description

Mechanics of Motor Proteins and the Cytoskeleton provides a physical foundation for molecular mechanics. Part I explains how small particles like proteins respond to mechanical, thermal, and chemical forces, Part II focuses on cytoskeletal filaments, and Part III focuses on motor proteins. The treatments are unified in the respect that they are organized around principles rather than proteins: chapters are centred on topics such as structure, chemistry, and mechanics, and different filaments or motors are discussed together.




Cytoskeletal Mechanics


Book Description

This book presents a full spectrum of views on current approaches to modeling cell mechanics. The authors come from the biophysics, bioengineering and physical chemistry communities and each joins the discussion with a unique perspective on biological systems. Consequently, the approaches range from finite element methods commonly used in continuum mechanics to models of the cytoskeleton as a cross-linked polymer network to models of glassy materials and gels. Studies reflect both the static, instantaneous nature of the structure, as well as its dynamic nature due to polymerization and the full array of biological processes. While it is unlikely that a single unifying approach will evolve from this diversity, it is the hope that a better appreciation of the various perspectives will lead to a highly coordinated approach to exploring the essential problems and better discussions among investigators with differing views.




The Cytoskeleton


Book Description




Cell Biology by the Numbers


Book Description

A Top 25 CHOICE 2016 Title, and recipient of the CHOICE Outstanding Academic Title (OAT) Award. How much energy is released in ATP hydrolysis? How many mRNAs are in a cell? How genetically similar are two random people? What is faster, transcription or translation?Cell Biology by the Numbers explores these questions and dozens of others provid




The Cytoskeleton


Book Description

The cytoskeleton is the intracellular filament system that controls the morphology of a cell, allows it to move, and provides trafficking routes for intracellular transport. It comprises three major filament systems-actin, microtubules, and intermediate filaments-along with a host of adaptors, regulators, molecular motors, and additional structural proteins. This textbook presents a comprehensive and up-to-date view of the cytoskeleton, cataloguing its many different components and explaining how they are functionally integrated in different cellular processes. It starts by laying out the basic molecular hardware, before describing in detail how these components are assembled in cells and linked to neighboring cells and the extracellular matrix to maintain tissue architecture. It then surveys the roles of the cytoskeleton in processes such as intracellular transport, cell motility, signal transduction, and cell division. The book is thus essential reading for students learning about intracellular structure. It also represents a vital reference for all cell and developmental biologists working in this field.




Advances in Molecular and Cell Biology


Book Description

The fourth volume of the Advances in Molecular and Cell Biology series. Cell biology is a rapidly-developing discipline, bringing together many separate biological sciences. The interrelations of cell structure and function at molecular and subcellular levels are the central theme of the series.




The Plant Cytoskeleton


Book Description

Plant cells house highly dynamic cytoskeletal networks of microtubules and actin microfilaments. They constantly undergo remodeling to fulfill their roles in supporting cell division, enlargement, and differentiation. Following early studies on structural aspects of the networks, recent breakthroughs have connected them with more and more intracellular events essential for plant growth and development. Advanced technologies in cell biology (live-cell imaging in particular), molecular genetics, genomics, and proteomics have revolutionized this field of study. Stories summarized in this book may inspire enthusiastic scientists to pursue new directions toward understanding functions of the plant cytoskeleton. The Plant Cytoskeleton is divided into three sections: 1) Molecular Basis of the Plant Cytoskeleton; 2) Cytoskeletal Reorganization in Plant Cell Division; and 3) The Cytoskeleton in Plant Growth and Development. This book is aimed at serving as a resource for anyone who wishes to learn about the plant cytoskeleton beyond ordinary textbooks.