Oomycete Genetics and Genomics


Book Description

This book brings together the knowledge from and tools for genetic and genomic research into oomycetes to help solve the problems this pathogen poses to crops and animals. Armed with the information presented here, researchers can use oomycete data to solve practical problems and gain insight into future areas of interest. Key Features: Offers an up-to-date coverage of research into oomycetes – which has advanced with biochemical and molecular analyses in recent years Helps researchers use oomycete data to solve practical problems, like damage to crop and animal resources Includes a section on interactions with animal hosts Offers perspective on future areas of research Assembles an international author base




Molecular Identification of Fungi


Book Description

Fungi enjoy great popularity in pharmaceutical, agricultural, and biotechnological applications. Recent advances in the decipherment of whole fungal genomes promise an acceleration of these trends. This timely book links scientists from different parts of the world who are interested in the molecular identification of fungi combined with the exploration of the fungal biodiversity in different ecosystems. It provides a compendium for scientists who rely on a rapid and reliable detection of fungal specimens in environmental as well as clinical resources in order to ensure the benefit of industrial and clinical applications. Chapters focus on the opportunities and limits of the molecular marker-mediated identification of fungi. Various methods, procedures and strategies are outlined. Furthermore, the book offers an update of the current progress in the development of fungal molecular techniques, and draws attention to potential and associated problems, as well as integrating theory and practice.




Genomics of Plant-Associated Fungi and Oomycetes: Dicot Pathogens


Book Description

This book describes how genomics has revolutionized our understanding of agriculturally important plant-associated fungi and oomycetes. It illustrates some fundamental discoveries about these eukaryotic microbes with regard to the overall structure of their genomes, their lifestyles and the molecular mechanisms that form the basis of their interactions with plants. Genomics has provided new insights into fungal lifestyles and led to practical advances in plant breeding and crop protection, such as predictions about the spread and evolution of new pathogens. This volume focuses on fungi and oomycetes that are typical dicot plant pathogens and includes: Sclerotinia sclerotiorum, Botrytis cinerea, Alternaria sp.,Verticillium alfalfae and Verticillium dahliae, Fusarium oxysporum, Phytophthora capsici, Phytophthora sojae, Phytophthora ramorum, Phytophthora infestans, Hyaloperonospora arabidopsidis.




Molecular Markers in Mycology


Book Description

The Kingdom fungi encompass a massive diversity of taxa with wide-ranging ecologies, life cycles, and morphologies ranging from unicellular aquatic chytrids to large mushrooms. Before molecular methods came in existence, taxonomists considered this Kingdom to be a member of the plant kingdom due to certain life styles like immobility and growth habitats. Molecular markers (also known as DNA markers), facilitated a better alternative method over traditional morphological methods, employed for the identification, characterization, and to understand the evolution of fungi. The morphological methods used for identification are mainly dependent on spore color or microscopic features whereas molecular markers are based on DNA polymorphism in the genomic organization. Phylogenetic studies reported in last decade, based on molecular markers, have reshaped the classification system of Kingdom fungi, which divided into one subkingdom, seven phyla, and ten subphyla. Recent advances in molecular mycology have opened the way for researchers to identify and characterize novel fungal species from unique environments. Mycology is concerned with the systematic study of fungi, including their genetic and biochemical properties, their use to humans as a source of medicine and food, as well as their dangers, such as poisoning and infections. In the 21st century with the development of DNA sequencing technologies and phylogenetic analysis based on molecular markers, new insights into fungal taxonomy were provided. This book contains a thorough discussion of molecular characterization and detection of different groups of fungi by using PCR-based markers and provides a comprehensive view of the applications and uses of different molecular markers in molecular mycology. It also addresses the recent molecular markers employed to solve the problems of identification and discusses current approaches used in molecular characterization and detection of fungi.




Plant Pathogenic Fungi and Oomycetes


Book Description

This book describes approaches for the analysis of plant pathogenic fungi that range from ecosystem composition to in vitro and wet-lab to computational analyses. Chapters detail bioinformatics protocols for genome assembly, transcriptome analysis and small RNA profiling, characterization of secondary metabolome, analysis of post-translational modification and localization of proteins, examination of virulence function and interaction with plants or other microorganisms, and targeted mutagenesis using various approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Pathogenic Fungi and Oomycetes: Methods and Protocols aims to provide techniques developed through a wide range of perspectives to facilitate research on a comprehensive understanding of these important filamentous pathogens.




Modern Assays for Plant Pathogenic Fungi


Book Description

This practically-oriented book provides both the theoretical background and step-by-step protocols for methods employing new technologies for the detection of plant pathogenic fungi. Methods have recently been developed which can be undertaken on a wide scale, with large numbers of samples, and which are simple to use, highly sensitive and very specific. Hybridoma and DNA technologies provide the basis of such assays and include the use of monoclonal antibodies, the polymerase chain reaction (PCR) and DNA hybridization. The book is based on papers presented at a conference held in Oxford in March 1993, under the BRIDGE-COST-88 program developed by the Commission of the European Communities. A wide range of fungi, which are pathogens of a variety of crop and ornamental plants, is covered and the book is aimed at postgraduate students and research workers in mycology and plant pathology, including those involved in routine testing of plants for quarantine or other purposes




Fungal Plant Pathogens, 2nd Edition


Book Description

This substantially updated edition now in full colour provides key techniques used when working with fungal and fungal-like plant pathogens. As a practical manual it also deals with disease recognition, detection and identification of fungi, plus methods to characterise and curate fungi and handle them under quarantine and quality assurance systems. Fungal Plant Pathogens: Applied Techniques, 2nd edition provides a valuable guide to investigating fungal plant diseases and interpreting laboratory findings for postgraduate and advanced undergraduate students, extension plant pathologists, consultants and advisers in agriculture, forestry and horticulture, and the food supply chain.




The Potato Crop


Book Description

This book is open access under a CC BY 4.0 license. This book provides a fresh, updated and science-based perspective on the current status and prospects of the diverse array of topics related to the potato, and was written by distinguished scientists with hands-on global experience in research aspects related to potato. The potato is the third most important global food crop in terms of consumption. Being the only vegetatively propagated species among the world’s main five staple crops creates both issues and opportunities for the potato: on the one hand, this constrains the speed of its geographic expansion and its options for international commercialization and distribution when compared with commodity crops such as maize, wheat or rice. On the other, it provides an effective insulation against speculation and unforeseen spikes in commodity prices, since the potato does not represent a good traded on global markets. These two factors highlight the underappreciated and underrated role of the potato as a dependable nutrition security crop, one that can mitigate turmoil in world food supply and demand and political instability in some developing countries. Increasingly, the global role of the potato has expanded from a profitable crop in developing countries to a crop providing income and nutrition security in developing ones. This book will appeal to academics and students of crop sciences, but also policy makers and other stakeholders involved in the potato and its contribution to humankind’s food security.




Fossil Fungi


Book Description

Fungi are ubiquitous in the world and responsible for driving the evolution and governing the sustainability of ecosystems now and in the past. Fossil Fungi is the first encyclopedic book devoted exclusively to fossil fungi and their activities through geologic time. The book begins with the historical context of research on fossil fungi (paleomycology), followed by how fungi are formed and studied as fossils, and their age. The next six chapters focus on the major lineages of fungi, arranging them in phylogenetic order and placing the fossils within a systematic framework. For each fossil the age and provenance are provided. Each chapter provides a detailed introduction to the living members of the group and a discussion of the fossils that are believed to belong in this group. The extensive bibliography (~ 2700 entries) includes papers on both extant and fossil fungi. Additional chapters include lichens, fungal spores, and the interactions of fungi with plants, animals, and the geosphere. The final chapter includes a discussion of fossil bacteria and other organisms that are fungal-like in appearance, and known from the fossil record. The book includes more than 475 illustrations, almost all in color, of fossil fungi, line drawings, and portraits of people, as well as a glossary of more than 700 mycological and paleontological terms that will be useful to both biologists and geoscientists. - First book devoted to the whole spectrum of the fossil record of fungi, ranging from Proterozoic fossils to the role of fungi in rock weathering - Detailed discussion of how fossil fungi are preserved and studied - Extensive bibliography with more than 2000 entries - Where possible, fungal fossils are placed in a modern systematic context - Each chapter within the systematic treatment of fungal lineages introduced with an easy-to-understand presentation of the main characters that define extant members - Extensive glossary of more than 700 entries that define both biological, geological, and mycological terminology