Liquid Culture Systems for in vitro Plant Propagation


Book Description

High-efficiency micropropagation, with relatively low labour costs, has been demonstrated in this unique book detailing liquid media systems for plant tissue culture. World authorities (e.g. von Arnold, Curtis, Takayama, Ziv) contribute seminal papers together with papers from researchers across Europe that are members of the EU COST Action 843 "Advanced micropropagation systems". First-hand practical applications are detailed for crops – including ornamentals and trees – using a wide range of techniques, from thin-film temporary immersion systems to more traditional aerated bioreactors with many types of explant – shoots to somatic embryos. The accounts are realistic, balanced and provide a contemporary account of this important aspect of mass propagation. This book is essential reading for all those in commercial micropropagation labs, as well as researchers worldwide who are keen to improve propagation techniques and lower economic costs of production. Undergraduate and postgraduate students in the applied plant sciences and horticulture will find the book an enlightened treatise.













Plant Propagation by Tissue Culture


Book Description

For researchers and students, George’s books have become the standard works on in vitro plant propagation. For this, the third edition of the classic work, authors with specialist knowledge have been brought on board to cover the hugely expanded number of topics in the subject area. Scientific knowledge has expanded rapidly since the second edition and it would now be a daunting task for a single author to cover all aspects adequately. However, this edition still maintains the integration that was characteristic of the previous editions. The first volume of the new edition highlights the scientific background of in vitro propagation. The second volume covers the practice of micropropagation and describes its various applications.




Plant Tissue Culture Manual


Book Description

Basic techniques - cells tissue culture of model species. Tissue culture & transformation of crop species. Propagation & conservation of germplasm. Direct gene transfer & protoplast fusion. Reproductive tissues. Mutant selection.




Modern Applications of Plant Biotechnology in Pharmaceutical Sciences


Book Description

Modern Applications of Plant Biotechnology in Pharmaceutical Sciences explores advanced techniques in plant biotechnology, their applications to pharmaceutical sciences, and how these methods can lead to more effective, safe, and affordable drugs. The book covers modern approaches in a practical, step-by-step manner, and includes illustrations, examples, and case studies to enhance understanding. Key topics include plant-made pharmaceuticals, classical and non-classical techniques for secondary metabolite production in plant cell culture and their relevance to pharmaceutical science, edible vaccines, novel delivery systems for plant-based products, international industry regulatory guidelines, and more. Readers will find the book to be a comprehensive and valuable resource for the study of modern plant biotechnology approaches and their pharmaceutical applications. Builds upon the basic concepts of cell and plant tissue culture and recombinant DNA technology to better illustrate the modern and potential applications of plant biotechnology to the pharmaceutical sciences Provides detailed yet practical coverage of complex techniques, such as micropropogation, gene transfer, and biosynthesis Examines critical issues of international importance and offers real-life examples and potential solutions




Micropropagation


Book Description

Micropropagation is a technology that has developed within the past 30 years. Earlier overviews of plant tissue culture have reviewed micropropagation as just one of many tissue culture procedures in use. Since the applications of this technology have multiplied so rapidly in recent years, we decided that a specific overview of the technology was now appropriate Our book begins with a review of the general principles of tissue culture as applied to micropropagation. This review is concise since the general topic has been covered in numerous other books and reviews. The basic principles of laboratory design and construction are summarized in the second chapter. Common problems encountered in micropropagation, both during and after culture, are examined in detail in four chapters. As micropropagation developed from a laboratory curiosity to a commercial industry, different considerations became important. These are discussed in two chapters. An attempt has been made to assess the current status of commercial production around the world. This has been difficult because commercial production figures are often closely guarded and little has been done to collect statistics on this growing industry. Applications to a broad range of crops are discussed in a series of chapters. These try to report the state of the art in each area, but since applications for some crops are much more advanced than for others, the focus of these chapters varies depending upon the progress that has been made.




In Vitro Cultivation of Plant Cells


Book Description

This text aims to provide the essential knowledge of the core processes involved in the cultivation of plant cells and tissues in vitro thereby enabling readers to understand and contribute to the practical application of these techniques. The text begins by introducing the key terms used in plant tissue culture and provides an overview of the range and objectives of plant tissue culture studies. Subsequent chapters provide knowledge of the processes of the major techniques developed within the framework of the perspectives described in the introductory chapter. The important issues of media formulation and preparation are described together with discussion of the influence of plant growth regulators on the growth and development of plant cell systems in vitro.




Somatic Embryogenesis and Synthetic Seed I


Book Description

While working in the laboratory of Professor Dr. Jacob Reinert at the Freie Universitat Berlin (1974-1976), I had the opportunity to become deeply involved in studying the intricacies of the fascinating phenomenon of somatic embryogenesis in plant cells and protoplasts. In numerous stimu lating discussions with Professor Reinert on this subject, I was fully convinced that somatic embryogenesis would become one of the most important areas of study, not only regarding basic and fundamental aspects, but also for its application in crop improvement. During the last decade, we have witnessed tremendous interest and achievements in the use of somatic embryos for the production of synthetic seeds, for micro propagation, genetic transformation, cryopreservation, and conservation of germplasm. The en masse production of somatic embryos in the bioreactors has facilitated some of these studies. Somatic embryos have now been induced in more than 300 plant species belonging to a wide range offamilies. It was therefore felt that a compilation ofliterature/state of the art on this subject was necessary. Thus, two volumes on Somatic Embryo genesis and Synthetic Seed have been compiled, which contain 65 chapters contributed by International experts. Somatic Embryogenesis and Synthetic Seed I comprises 31 chapters, arranged in 3 sections: Section I Commitment of the cell to somatic embryogenesis; early events; anatomy; molecular basis; gene expression; role of polyamines; machine vision analysis of somatic embryos. Section II Applications of somatic embryos; technology of synthetic seed; fluid drilling; micropropagation; genetic transfor mation through somatic embryos; cryopreservation.