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Microarrays in Clinical Diagnostics

Author : Thomas O. Joos

Publisher : Springer Science & Business Media

Page : 283 pages

File Size : 50,10 MB

Release : 2008-02-02

Category : Medical

ISBN : 1592599230

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Book Description

Leading academic and industrial investigators surveys the world of microarray technology, describing in step-by-step detail diverse DNA and protein assays in clinical laboratories using state-of-the-art technologies. The advanced tools and methods described are designed for mRNA expression analysis, SNP analysis, identification, and quantification of proteins, and for studies of protein-protein interactions. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.



Molecular Microbiology

Author : David H. Persing

Publisher : John Wiley & Sons

Page : 852 pages

File Size : 25,75 MB

Release : 2020-07-24

Category : Science

ISBN : 1555819079

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Book Description

Presenting the latest molecular diagnostic techniques in one comprehensive volume The molecular diagnostics landscape has changed dramatically since the last edition of Molecular Microbiology: Diagnostic Principles and Practice in 2011. With the spread of molecular testing and the development of new technologies and their opportunities, laboratory professionals and physicians more than ever need a resource to help them navigate this rapidly evolving field. Editors David Persing and Fred Tenover have brought together a team of experienced researchers and diagnosticians to update this third edition comprehensively, to present the latest developments in molecular diagnostics in the support of clinical care and of basic and clinical research, including next-generation sequencing and whole-genome analysis. These updates are provided in an easy-to-read format and supported by a broad range of practical advice, such as determining the appropriate type and quantity of a specimen, releasing and concentrating the targets, and eliminating inhibitors. Molecular Microbiology: Diagnostic Principles and Practice Presents the latest basic scientific theory underlying molecular diagnostics Offers tested and proven applications of molecular diagnostics for the diagnosis of infectious diseases, including point-of-care testing Illustrates and summarizes key concepts and techniques with detailed figures and tables Discusses emerging technologies, including the use of molecular typing methods for real-time tracking of infectious outbreaks and antibiotic resistance Advises on the latest quality control and quality assurance measures Explores the increasing opportunities and capabilities of information technology Molecular Microbiology: Diagnostic Principles and Practice is a textbook for molecular diagnostics courses that can also be used by anyone involved with diagnostic test selection and interpretation. It is also a useful reference for laboratories and as a continuing education resource for physicians. If you are looking for online access to the latest clinical microbiology content, please visit www.wiley.com/learn/clinmicronow.



Microarray Technology and Its Applications

Author : Uwe R. Müller

Publisher : Springer Science & Business Media

Page : 388 pages

File Size : 29,45 MB

Release : 2006-03-30

Category : Medical

ISBN : 3540265783

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Book Description

Ithasbeenstatedthatourknowledgedoublesevery20years,butthatmaybe an understatement when considering the Life Sciences. A series of discoveries and inventions have propelled our knowledge from the recognition that DNA isthegeneticmaterialtoabasicmolecularunderstandingofourselvesandthe living world around us in less than 50 years. Crucial to this rapid progress was thediscoveryofthedouble-helicalstructureofDNA,whichlaidthefoundation forallhybridizationbasedtechnologies. Thediscoveriesofrestrictionenzymes, ligases, polymerases, combined with key innovations in DNA synthesis and sequencing ushered in the era of biotechnologyas a new science with profound sociological and economic implications that are likely to have a dominating in?uence on the development of our society during this century. Given the process by which science builds on prior knowledge, it is perhaps unfair to single out a few inventions and credit them with having contributed most to thisavalancheofknowledge. Yet,therearesurelysomethatwillberecognized as having had a more profound impact than others, not just in the furthering of our scienti?c knowledge, but by leveraging commercial applications that provide a tangible return to our society. The now famous Polymerase Chain Reaction, or PCR, is surely one of those, as it has uniquely catalyzed molecular biology during the past 20 years, and continues to have a signi?cant impact on all areas that involve nucleic acids, ranging from molecular pathology to forensics. Ten years ago micro- ray technology emerged as a new and powerful tool to study nucleic acid - quences in a highly multiplexed manner, and has since found equally exciting and useful applications in the study of proteins, metabolites, toxins, viruses, whole cells and even tissues.



Microarray Innovations

Author : Gary Hardiman

Publisher : CRC Press

Page : 362 pages

File Size : 29,13 MB

Release : 2009-04-02

Category : Medical

ISBN : 1420094513

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Book Description

In recent years, high-density DNA microarrays have revolutionized biomedical research and drug discovery efforts by the pharmaceutical industry. Their efficacy in identifying and prioritizing drug targets based on their ability to confirm a large number of gene expression measurements in parallel has become a key element in drug discovery. Microarr



A Beginner’s Guide to Microarrays

Author : Eric M. Blalock

Publisher : Springer Science & Business Media

Page : 356 pages

File Size : 32,23 MB

Release : 2011-06-27

Category : Science

ISBN : 1441987606

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Book Description

A Beginner's Guide to Microarrays addresses two audiences - the core facility manager who produces, hybridizes, and scans arrays, and the basic research scientist who will be performing the analysis and interpreting the results. User friendly coverage and detailed protocols are provided for the technical steps and procedures involved in many facets of microarray technology, including: -Cleaning and coating glass slides, -Designing oligonucleotide probes, -Constructing arrays for the detection and quantification of different bacterial species, -Preparing spotting solutions, -Troubleshooting spotting problems, -Setting up and running a core facility, -Normalizing background signal and controlling for systematic variance, -Designing experiments for maximum effect, -Analyzing data with statistical procedures, -Clustering data with machine-learning protocols.



Protein Microarray Technology

Author : Dev Kambhampati

Publisher : Wiley-Blackwell

Page : 284 pages

File Size : 12,73 MB

Release : 2004-03

Category : Medical

ISBN :

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Book Description

Protein microarrays soon will become one of the major tools for realizing the goals of drug discovery research in the post-genome era. The book is aimed at life science researchers and scientists working in drug and pharma industry.



Genomic Medicine

Author : Dhavendra Kumar

Publisher : Oxford Monographs on Medical G

Page : 853 pages

File Size : 10,80 MB

Release : 2014-10-15

Category : Medical

ISBN : 019989602X

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Book Description

Preceded by Genomics and clinical medicine / edited by Dhavendra Kumar. [First edition]. 2008.



Single Cell Diagnostics

Author : Alan R. Thornhill

Publisher : Springer Science & Business Media

Page : 185 pages

File Size : 13,59 MB

Release : 2008-02-02

Category : Science

ISBN : 159745298X

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Book Description

This book applies modern molecular diagnostic techniques to the analysis of single cells, small numbers of cells, or cell extracts. Emphasis is placed on non-invasive analysis of single cell metabolites and the direct analysis of RNA and DNA from single cells, with a focus on polymerase chain reaction and fluorescence in situ hybridization. In particular, this handbook is essential for practitioners providing care for couples seeking treatment for infertility.



Microchip Methods in Diagnostics

Author : Ursula Bilitewski

Publisher : Humana Press

Page : 183 pages

File Size : 28,26 MB

Release : 2008-12-18

Category : Science

ISBN : 9781588299550

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Book Description

In this new age of science and technology, microchip methods of analysis have developed into an essential tool for biomedical researchers. Microchip Methods in Diagnostics looks at this increasingly miniaturized technology, focusing on DNA microarrays, protein microarrays, and labs-on-chips, and supporting the introduction of new diagnostic methods based on microtechnologies. The work provides a background on the technological aspects of each of these methods, followed by chapters describing the methods with regard to specific applications. Since it uses only those processes which have reached a certain level of maturation, this volume is able to provide approaches that are applicable in practice even by the non-expert. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. An innovative and cutting-edge work, Microchip Methods in Diagnostics strives to bring the technology of microchips even further into the world of diagnostics, predicting incredible benefits for patients in the years to come.



Proteomics for Biological Discovery

Author : Timothy D. Veenstra

Publisher : John Wiley & Sons

Page : 361 pages

File Size : 36,9 MB

Release : 2006-06-12

Category : Science

ISBN : 0470007737

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Book Description

Written by recognized experts in the study of proteins, Proteomics for Biological Discovery begins by discussing the emergence of proteomics from genome sequencing projects and a summary of potential answers to be gained from proteome-level research. The tools of proteomics, from conventional to novel techniques, are then dealt with in terms of underlying concepts, limitations and future directions. An invaluable source of information, this title also provides a thorough overview of the current developments in post-translational modification studies, structural proteomics, biochemical proteomics, microfabrication, applied proteomics, and bioinformatics relevant to proteomics. Presents a comprehensive and coherent review of the major issues faced in terms of technology development, bioinformatics, strategic approaches, and applications Chapters offer a rigorous overview with summary of limitations, emerging approaches, questions, and realistic future industry and basic science applications Discusses higher level integrative aspects, including technical challenges and applications for drug discovery Accessible to the novice while providing experienced investigators essential information Proteomics for Biological Discovery is an essential resource for students, postdoctoral fellows, and researchers across all fields of biomedical research, including biochemistry, protein chemistry, molecular genetics, cell/developmental biology, and bioinformatics.