Molecular Detection and Identification of Parasites Involved in Human Disease


Book Description

With increased sensitivity, a less invasive sampling procedure would be required from the patient. Also, the earlier the presence of parasites in the tissues is detected, the earlier the diagnosis would occur. Tissues that contain very few parasites and had been classified as previously unusable for diagnosis or tissues from older medical cases can be analyzed for the presence of disease and the identification of causative species. The successful application of the singleplex PCR technique to Plasmodium falciparum and Leishmania degraded DNA samples leads to the feasibility of success for the multiplex PCR techniques involving degraded DNA analyses. Therefore, this methodology may be applied to samples of unknown disease content to either include or exclude the agents of disease. Future directions may extend these analyses to include a range of other agents of disease, thus allowing for the determination of disease antiquity through degraded DNA analyses."--Proquest theses abstract.




Molecular Detection of Human Parasitic Pathogens


Book Description

Traditionally, laboratory identification of parasites has relied upon various phenotypic procedures that detect their morphological, biological, and immunological features. Because these procedures tend to be time-consuming and technically demanding, molecular methods based on nucleic acid amplification technologies have been increasingly utilized for rapid, sensitive, and specific characterization of parasites. The large number of original and modified molecular protocols that have been developed over the years creates a dilemma for those attempting to adopt the most appropriate protocol for streamlined identification and detection of human pathogenic organisms of interest. Part of a four-volume collection, Molecular Detection of Human Parasitic Pathogens provides a reliable and comprehensive resource on the molecular detection and identification of major human parasitic pathogens. This volume contains expert contributions from international scientists involved in human parasitic pathogen research and diagnosis. Following a similar format throughout, each chapter includes: A brief review on the classification, biology, epidemiology, clinical features, and diagnosis of an important pathogenic parasitic genus/group An outline of clinical sample collection and preparation procedures and a selection of representative stepwise molecular protocols A discussion on further research needs relating to improved diagnoses of major human parasitic pathogens This versatile reference on molecular detection and identification of major human parasitic pathogens is an indispensable tool for upcoming and experienced medical, veterinary, and industrial laboratory scientists engaged in parasite characterization. It is also suitable as a textbook for undergraduate and graduate students majoring in parasitology.




Microbial Threats to Health


Book Description

Infectious diseases are a global hazard that puts every nation and every person at risk. The recent SARS outbreak is a prime example. Knowing neither geographic nor political borders, often arriving silently and lethally, microbial pathogens constitute a grave threat to the health of humans. Indeed, a majority of countries recently identified the spread of infectious disease as the greatest global problem they confront. Throughout history, humans have struggled to control both the causes and consequences of infectious diseases and we will continue to do so into the foreseeable future. Following up on a high-profile 1992 report from the Institute of Medicine, Microbial Threats to Health examines the current state of knowledge and policy pertaining to emerging and re-emerging infectious diseases from around the globe. It examines the spectrum of microbial threats, factors in disease emergence, and the ultimate capacity of the United States to meet the challenges posed by microbial threats to human health. From the impact of war or technology on disease emergence to the development of enhanced disease surveillance and vaccine strategies, Microbial Threats to Health contains valuable information for researchers, students, health care providers, policymakers, public health officials. and the interested public.







Global Infectious Disease Surveillance and Detection


Book Description

Early detection is essential to the control of emerging, reemerging, and novel infectious diseases, whether naturally occurring or intentionally introduced. Containing the spread of such diseases in a profoundly interconnected world requires active vigilance for signs of an outbreak, rapid recognition of its presence, and diagnosis of its microbial cause, in addition to strategies and resources for an appropriate and efficient response. Although these actions are often viewed in terms of human public health, they also challenge the plant and animal health communities. Surveillance, defined as "the continual scrutiny of all aspects of occurrence and spread of a disease that are pertinent to effective control", involves the "systematic collection, analysis, interpretation, and dissemination of health data." Disease detection and diagnosis is the act of discovering a novel, emerging, or reemerging disease or disease event and identifying its cause. Diagnosis is "the cornerstone of effective disease control and prevention efforts, including surveillance." Disease surveillance and detection relies heavily on the astute individual: the clinician, veterinarian, plant pathologist, farmer, livestock manager, or agricultural extension agent who notices something unusual, atypical, or suspicious and brings this discovery in a timely way to the attention of an appropriate representative of human public health, veterinary medicine, or agriculture. Most developed countries have the ability to detect and diagnose human, animal, and plant diseases. Global Infectious Disease Surveillance and Detection: Assessing the Challenges-Finding Solutions, Workshop Summary is part of a 10 book series and summarizes the recommendations and presentations of the workshop.




Human Parasites: From Organisms To Molecular Biology


Book Description

Why does the World Health Organization (WHO) put emphasis on neglected tropical diseases (NTDs)? What are the NTDs? Are NTDs found in the United States? Is there any relationship between coronavirus disease 2019 (COVID-19) and NTDs? These are some of the questions being addressed in the book.The aim of this textbook is to introduce a modern synthesis on human parasites of medical importance. Species of parasitic protozoa and helminths are presented in detail, from history and discovery to aspects of genomes and molecular biology, together with life cycle, therapy, drug resistance, and case studies of parasitic diseases useful to the clinicians.




Molecular Detection of Human Bacterial Pathogens


Book Description

As more original molecular protocols and subsequent modifications are described in the literature, it has become difficult for those not directly involved in the development of these protocols to know which are most appropriate to adopt for accurate identification of bacterial pathogens. Molecular Detection of Human Bacterial Pathogens addresses th




Parasites, a Guide to Laboratory Procedures and Identification


Book Description

This manual has been developed to provide those who are involved in the diagnosis of parasitic diseases with a resource that encompasses the basic technology required for detection of parasites and identification of their diagnostic stages in feces, blood, other body fluids, and tissues. In addition to the technical procedures described, a summary of the morphologic charateristics that are used in the identification of protozoa and helminths is provided as well as line drawings, original color representations of the stages of the human malarial parasites, and photomicrographs of the diagnostic stages of many parasites.




Blood and Tissue Parasites


Book Description

Examines parasites that cause malaria, babesiosis, trypanosomiasis, leishmaniasis, toxoplasmosis, pneumocytosis and filariasis.




Laboratory Diagnosis of Infectious Diseases


Book Description

Designed for associate-degree MLT/CLT programs and baccalaureate MT/CLS programs, this textbook presents the essentials of clinical microbiology. It provides balanced coverage of specific groups of microorganisms and the work-up of clinical specimens by organ system, and also discusses the role of the microbiology laboratory in regard to emerging infections, healthcare epidemiology, and bioterrorism. Clinical case studies and self-assessment questions show how to incorporate the information into everyday practice. More than 400 illustrations and visual information displays enhance the text. Essentials boxes, chapter outlines, key terms, summaries, and other study aids help students retain information. A bound-in CD-ROM includes additional review questions, case studies, and Web links.