› › Monitoring Proteolytic Cleavage Of A Membrane Metalloproteinase On The Cell Surface With A Cell Based Assay

Protein Sequencing and Identification Using Tandem Mass Spectrometry

Author : Michael Kinter

Publisher : John Wiley & Sons

Page : 321 pages

File Size : 47,19 MB

Release : 2005-04-12

Category : Science

ISBN : 0471231886

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Book Description

How to design, execute, and interpret experiments for protein sequencing using mass spectrometry The rapid expansion of searchable protein and DNA databases in recent years has triggered an explosive growth in the application of mass spectrometry to protein sequencing. This timely and authoritative book provides professionals and scientists in biotechnology research with complete coverage of procedures for analyzing protein sequences by mass spectrometry, including step-by-step guidelines for sample preparation, analysis, and data interpretation. Michael Kinter and Nicholas Sherman present their own high-quality, laboratory-tested protocols for the analysis of a wide variety of samples, demonstrating how to carry out specific experiments and obtain fast, reliable results with a 99% success rate. Readers will get sufficient experimental detail to apply in their own laboratories, learn about the proper selection and operation of instruments, and gain essential insight into the fundamental principles of mass spectrometry and protein sequencing. Coverage includes: * Peptide fragmentation and interpretation of product ion spectra * Basic polyacrylamide gel electrophoresis * Preparation of protein digests for sequencing experiments * Mass spectrometric analysis using capillary liquid chromatography * Techniques for protein identification by database searches * Characterization of modified peptides using tandem mass spectrometry And much more



Extracellular Matrix Degradation

Author : William C. Parks

Publisher : Springer Science & Business Media

Page : 262 pages

File Size : 16,79 MB

Release : 2011-04-07

Category : Science

ISBN : 3642168612

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Book Description

Regulated turnover of extracellular matrix (ECM) is an important component of tissue homeostasis. In recent years, the enzymes that participate in, and control ECM turnover have been the focus of research that touches on development, tissue remodeling, inflammation and disease. This volume in the Biology of Extracellular Matrix series provides a review of the known classes of proteases that degrade ECM both outside and inside the cell. The specific EMC proteases that are discussed include cathepsins, bacterial collagenases, matrix metalloproteinases, meprins, serine proteases, and elastases. The volume also discusses the domains responsible for specific biochemical characteristics of the proteases and the physical interactions that occur when the protease interacts with substrate. The topics covered in this volume provide an important context for understanding the role that matrix-degrading proteases play in normal tissue remodeling and in diseases such as cancer and lung disease.



Nanoplasmonic Sensors

Author : Alexandre Dmitriev

Publisher : Springer Science & Business Media

Page : 394 pages

File Size : 29,43 MB

Release : 2012-07-31

Category : Science

ISBN : 1461439337

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Book Description

This book is a compendium of the finest research in nanoplasmonic sensing done around the world in the last decade. It describes basic theoretical considerations of nanoplasmons in the dielectric environment, gives examples of the multitude of applications of nanoplasmonics in biomedical and chemical sensing, and provides an overview of future trends in optical and non-optical nanoplasmonic sensing. Specifically, readers are guided through both the fundamentals and the latest research in the two major fields nanoplasmonic sensing is applied to – bio- and chemo-sensing – then given the state-of-the-art recipes used in nanoplasmonic sensing research.



How Tobacco Smoke Causes Disease

Author : United States. Public Health Service. Office of the Surgeon General

Publisher :

Page : 728 pages

File Size : 42,1 MB

Release : 2010

Category : Government publications

ISBN :

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Book Description

This report considers the biological and behavioral mechanisms that may underlie the pathogenicity of tobacco smoke. Many Surgeon General's reports have considered research findings on mechanisms in assessing the biological plausibility of associations observed in epidemiologic studies. Mechanisms of disease are important because they may provide plausibility, which is one of the guideline criteria for assessing evidence on causation. This report specifically reviews the evidence on the potential mechanisms by which smoking causes diseases and considers whether a mechanism is likely to be operative in the production of human disease by tobacco smoke. This evidence is relevant to understanding how smoking causes disease, to identifying those who may be particularly susceptible, and to assessing the potential risks of tobacco products.



Matrix Metalloproteinases and TIMPs

Author : J. F. Woessner

Publisher :

Page : 0 pages

File Size : 29,1 MB

Release : 2023

Category : Extracellular matrix proteins

ISBN : 9781383020427

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Book Description

This study covers the sequence information, three-dimensional structures, activation, protein substrates, specificity requirements, inhibition, and biological roles of identified MMPs.



Plant Proteases

Author : Mercedes Diaz-Mendoza

Publisher : Frontiers Media SA

Page : 157 pages

File Size : 39,61 MB

Release : 2020-01-24

Category :

ISBN : 2889633993

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Book Description

Plant proteases are involved in most aspects of plant physiology and development, playing key roles in the generation of signaling molecules and as regulators of essential cellular processes such as cell division and metabolism. They take part in important pathways like protein turnover by the degradation of misfolded proteins and the ubiquitin-proteasome pathway, and they are responsible for post-translational modifications of proteins by proteolysis at highly specific sites. Proteases are also implicated in a great variety of environmentally controlled processes, including mobilization of storage proteins during seed germination, development of seedlings, senescence, programmed cell death and defense mechanisms against pests and pathogens. However, in spite of their importance, little is known about the functions and mode of actions of specific plant proteases. This Research Topic collects contributions covering diverse aspects of plant proteases research.



Rab GTPases

Author : Guangpu Li (Molecular biologist)

Publisher :

Page : 310 pages

File Size : 20,34 MB

Release : 2021

Category : Guanosine triphosphatase

ISBN : 9781071613467

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Book Description

This second edition volume expands on the previous edition with a discussion of new research and discoveries in the Rab field. Chapters in this book cover topics such as new information on Rab regulation and localization; interaction; function; and diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Rab GTPases: Methods and Protocols, Second Edition is a valuable resource for scientists working in the fields of Rab and other small GTPases, and beyond.



Snake Venom Metalloproteinases

Author : Jay Fox

Publisher : MDPI

Page : 277 pages

File Size : 46,64 MB

Release : 2018-07-10

Category : Science

ISBN : 3038424269

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Book Description

This book is a printed edition of the Special Issue "Snake Venom Metalloproteinases" that was published in Toxins



Innovations for Next-Generation Antibody-Drug Conjugates

Author : Marc Damelin

Publisher : Springer

Page : 358 pages

File Size : 48,29 MB

Release : 2018-05-29

Category : Medical

ISBN : 3319781545

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Book Description

Antibody-drug conjugates (ADCs) stand at the verge of a transformation. Scores of clinical programs have yielded only a few regulatory approvals, but a wave of technological innovation now empowers us to overcome past technical challenges. This volume focuses on the next generation of ADCs and the innovations that will enable them. The book inspires the future by integrating the field’s history with novel strategies and cutting-edge technologies. While the book primarily addresses ADCs for solid tumors, the last chapter explores the emerging interest in using ADCs to treat other diseases. The therapeutic rationale of ADCs is strong: to direct small molecules to the desired site of action (and away from normal tissues) by conjugation to antibodies or other targeting moieties. However, the combination of small and large molecules imposes deep complexity to lead optimization, pharmacokinetics, toxicology, analytics and manufacturing. The field has made significant advances in all of these areas by improving target selection, ADC design, manufacturing methods and clinical strategies. These innovations will inspire and educate scientists who are designing next-generation ADCs with the potential to transform the lives of patients.



Pathophysiological Aspects of Proteases

Author : Sajal Chakraborti

Publisher : Springer

Page : 668 pages

File Size : 12,32 MB

Release : 2017-11-15

Category : Medical

ISBN : 9811061416

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Book Description

This book provides a comprehensive overview of the multifaceted field of protease in the cellular environment and focuses on the recently elucidated functions of complex proteolytic systems in physiology and pathophysiology. Given the breadth and depth of information covered in the respective contributions, the book will be immensely useful for researchers working to identify targets for drug development. Multidisciplinary in scope, the book bridges the gap between fundamental and translational research, with applications in the biomedical and pharmaceutical industry, making it a thought-provoking read for basic and applied scientists engaged in biomedical research. Proteases represent one of the largest and most diverse families of enzymes known, and we now know that they are involved in every aspect of a given organism’s life functions. Under physiological conditions, proteases are regulated by their endogenous inhibitors. However, when the activity of proteases is not correctly regulated, disease processes such as tumour progression, vascular remodelling, atherosclerotic plaque progression, ulcer, rheumatoid arthritis, Alzheimer’s disease and inflammation can result. Many infective microorganisms require proteases for replication or use them as virulence factors, which has facilitated the development of protease-targeted therapies for a variety of parasitic diseases.