Protocols for Micropropagation of Selected Economically-Important Horticultural Plants


Book Description

Micropropagation is a reliable technology applied commercially worldwide for large-scale plant multiplication, germplasm conservation, pathogen elimination, genetic manipulations and supply of selected plants. In Protocols for Micropropagation of Selected Economically-Important Horticultural Plants, well recognised researchers in the field compile step-wise protocols for rapid plant multiplication of economically-important horticultural species. The book contains 35 chapters, divided into four major sections. The first three sections (Section A, B and C) contain 29 micropropagation protocols of selected fruit and nut species, indoor and outdoor ornamental plants, cut flowers, and vegetables. In addition to the detailed protocols of in vitro shoot initiation, proliferation, root induction and acclimatization, chapters also include detailed information on medium preparation, explant selection and preparation. The six chapters of Section D cover specific reviews on pivotal topics, such as in vitro rejuvenation, synthetic seed technology, thermotherapy and meristem culture in banana, genetic transformation of pineapple, flower color somaclonal variation in torenia, and cryotherapy of horticultural crops. Moreover, as a part of the highly successful Methods in Molecular Biology series, chapters include introductions to the respective topic, lists of necessary materials, notes, and illustrative photos. Comprehensive and well-written, Protocols for Micropropagation of Selected Economically-Important Horticultural Plants offers a useful resource for horticulturists, researchers, commercial companies, plant propagators, biotechnologists and students interested in micropropagation.




Protocols for Micropropagation of Selected Economically-Important Horticultural Plants


Book Description

Micropropagation is a reliable technology applied commercially worldwide for large-scale plant multiplication, germplasm conservation, pathogen elimination, genetic manipulations and supply of selected plants. In Protocols for Micropropagation of Selected Economically-Important Horticultural Plants, well recognised researchers in the field compile step-wise protocols for rapid plant multiplication of economically-important horticultural species. The book contains 35 chapters, divided into four major sections. The first three sections (Section A, B and C) contain 29 micropropagation protocols of selected fruit and nut species, indoor and outdoor ornamental plants, cut flowers, and vegetables. In addition to the detailed protocols of in vitro shoot initiation, proliferation, root induction and acclimatization, chapters also include detailed information on medium preparation, explant selection and preparation. The six chapters of Section D cover specific reviews on pivotal topics, such as in vitro rejuvenation, synthetic seed technology, thermotherapy and meristem culture in banana, genetic transformation of pineapple, flower color somaclonal variation in torenia, and cryotherapy of horticultural crops. Moreover, as a part of the highly successful Methods in Molecular Biology series, chapters include introductions to the respective topic, lists of necessary materials, notes, and illustrative photos. Comprehensive and well-written, Protocols for Micropropagation of Selected Economically-Important Horticultural Plants offers a useful resource for horticulturists, researchers, commercial companies, plant propagators, biotechnologists and students interested in micropropagation.




Plant Tissue Culture


Book Description

Plant Tissue Culture In One Form Or Another Has Become One Of The Most Promising Branches Of Plant Science. Arising From The Totipotency Of Plant Cells, It Now Occupies A Key Position In Plant Breeding, Plant Propagation And Plant Biotechnology.Plant Tissue Culture - Basic And Applied Brings To The Student Accessible, Up-To-Date Information On This Subject. Basic Knowledge Of Tissue Culture Methods Such As Isolation Of Suitable Tissues From The Mother Plant, Maintenance Of The Tissues Under In Vitro Condition In An Undifferentiated Or De-Differentiated Stage, Methods Of Genetic Engineering And Gene Transfer, Chromosomal Studies And The Handling Of In Vitro Micro Plants Are Described In Detail In This Book. Similarly, Application Aspects Of Micropropagation, Haploid Cell Culture, Protoplast Culture, Embryo Culture, Somatic Embryogenesis And Artificial Seeds Are Also Discussed.




Protocols for Micropropagation of Woody Trees and Fruits


Book Description

Micropropagation has become a reliable and routine approach for large-scale rapid plant multiplication, which is based on plant cell, tissue and organ culture on well defined tissue culture media under aseptic conditions. A lot of research efforts are being made to develop and refine micropropagation methods and culture media for large-scale plant multiplication of several number of plant species. However, many forest and fruit tree species still remain recalcitrant to in vitro culture and require highly specific culture conditions for plant growth and development. The recent challenges on plant cell cycle regulation and the presented potential molecular mechanisms of recalcitrance are providing excellent background for understanding on totipotency and what is more development of micropropagation protocols. For large-scale in vitro plant production the important attributes are the quality, cost effectiveness, maintenance of genetic fidelity, and long-term storage. The need for appropriate in vitro plant regeneration methods for woody plants, including both forest and fruit trees, is still overwhelming in order to overcome problems facing micropropagation such as somaclonal variation, recalcitrant rooting, hyperhydricity, polyphenols, loss of material during hardening and quality of plant material. Moreover, micropropagation may be utilized, in basic research, in production of virus-free planting material, cryopreservation of endangered and elite woody species, applications in tree breeding and reforestation.




Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants


Book Description

World population is increasing at an alarming rate and this has resulted in increasing tremendously the demand for tree products such as wood for construction materials, fuel and paper, fruits, oils and medicines etc. This has put immense pressure on the world’s supplies of trees and raw material to industry and will continue to do so as long as human population continues to grow. Also, the quality of human diet, especially nutritional components, is adversely affected due to limited genetic improvement of most of fruit trees. Thus there is an immediate need to increase productivity of trees. Improvement has been made through conventional breeding methods, however, conventional breeding is very slow due to long life cycle of trees. A basic strategy in tree improvement is to capture genetic gain through clonal propagation. Clonal propagation via organogenesis is being used for the production of selected elite individual trees. However, the methods are labour intensive, costly, and produce low volumes. Genetic gain can now be captured through somatic embryogenesis. Formation of embryos from somatic cells by a process resembling zygotic embryogenesis is one of the most important features of plants. In 1958, Reinert in Germany and Steward in USA independently reported somatic embryogenesis in carrot cultures. Since then, tremendous progress in somatic embryogenesis of woody and non-woody plants has taken place. It offers a potentially large-scale propagation system for superior clones.




Innovation in Propagation of Fruit, Vegetable and Ornamental Plants


Book Description

In horticulture, plant propagation plays an important role, as the number of plants can be rapidly multiplied, retaining the desirable characteristics of the mother plants, and shortening the bearing age of plants. There are two primary forms of plant propagation: sexual and asexual. In nature, the propagation of plants most often involves sexual reproduction, and this form is still used in several species. Over the years, horticulturists have developed asexual propagation methods that use vegetative plant parts. Innovation in plant propagation has supported breeding programs and allowed the production of high quality nursery plants with the same genetic characteristics of the mother plant, free of diseases or pests.




Micropropagation of Ornamental Plants


Book Description

Micropropagation through an in vitro culture technique is a reliable biotechnological tool that holds a better scope and future for the propagation of numerous economically prominent ornamentals, including those having value in the foliage and flowering plants industry. As a lucrative alternative approach to conventional propagation, micropropagation is a highly refined, well-developed and suitable method to produce a large scale of progeny plants which are genetically identical to the stock plant in a short time. Micropropagation of ornamental plants is increasingly and continuously involved in the scientific progress of plant biotechnology and has a relevant horticultural impact. Accordingly, a new book on micropropagation of ornamental plants is always required. Microprpagation of Ornamental Plants: Research and Practice consists of nine chapters divided into four sections authored by experienced and prestigious authors from different parts of the globe. The editor and authors are recognized as eminent scientists in their fields, and they add the quality and credibility to the content. Each chapter is illustrated and attempts to convey the practical as well as the theoretical aspects of microproparagtion. This anthology combines smartly refined micropropagation protocols of a considerable number of economically important ornamental species. Furthermore, the book will serve as a reference for horticulturists, researchers, lecturers, and students in horticulture, agriculture and biotechnology.




Biotechnology of Fruit and Nut Crops, 2nd Edition


Book Description

This book covers the biotechnology of all the major fruit and nut species. Since the very successful first edition of this book in 2004, there has been rapid progress for many fruit and nut species in cell culture, genomics and genetic transformation, especially for citrus and papaya. This book covers both these cutting-edge technologies and regeneration pathways, protoplast culture, in vitro mutagenesis, ploidy manipulation techniques that have been applied to a wider range of species. Three crop species, Diospyros kaki (persimmon), Punica granatum (pomegranate) and Eriobotrya japonica (loquat) are included for the first time. The chapters are organized by plant family to make it easier to make comparisons and exploitation of work with related species. Each chapter discusses the plant family and the related wild species for 38 crop species, and has colour illustrations. It is essential for scientists and post graduate students who are engaged in the improvement of fruit, nut and plantation crops.




Protocols for In Vitro Propagation of Ornamental Plants


Book Description

Providing crucial information for the expanding ornamental plant industry, leading researchers in the field compile comprehensive and step-wise protocols for rapid plant multiplication and in vitro storage of major commercially viable ornamental plants.




The Olive


Book Description

The European or Mediterranean cultivated olive (Olea europaea L., subsp. europaea, var. europaea) is an ancient crop notable for its early domestication. Today, hundreds of olive varieties are grown to produce high-quality fruit for oil and table olives for human consumption. Over the last 30 years, the olive industry has undergone profound innovation due to scientific and technical advances, particularly in genomics, breeding, orchard management, mechanization and agro-ecology. Not all these developments are currently available to smaller producers. Outside the Mediterranean Basin, where it has been present for over 6,000 years, olive cultivation has spread to many other countries. These new olive-growing areas are helping further the expansion of the industry, due to increased awareness of the nutritional and health properties of extra virgin olive oil. The Olive: Botany and Production is an invaluable resource for researchers and students in horticulture and agriculture, in addition to producers involved in olive orchard management.