Protein Tyrosine Phosphatases


Book Description

This second edition volume expands on the previous edition with discussions on the latest advancements in protein tyrosine phosphatases (PTP) research used to investigate these essential enzymes and new inhibitors. The new techniques covered in the chapters of this book include studying enzymes in vitro, in cells, and in animal models through proteomics, genomics, and structural biology. Furthermore, new advances in pharmacology and drug design have contributed to the developing novel therapeutics that target PTPs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Protein Tyrosine Phosphatases: Methods and Protocols, Second Edition is a valuable resource for both experienced and novel researchers in this field, and will lead to discoveries and accelerated progress in the field of PTP, signal transduction, and drug development.







Plant Peroxisomes


Book Description

In the two decades since the last comprehensive work on plant peroxisomes appeared, the scientific approaches employed in the study of plant biology have changed beyond all recognition. The accelerating pace of plant research in the post-genomic era is leading us to appreciate that peroxisomes have many important roles in plant cells, including reserve mobilisation, nitrogen assimilation, defence against stress, and metabolism of plant hormones, which are vital for productivity and normal plant development. Many plant scientists are finding, and will no doubt continue to find, that their own area of research is connected in some way to peroxisomes. Written by the leading experts in the field, this book surveys peroxisomal metabolic pathways, protein targeting and biogenesis of the organelle and prospects for the manipulation of peroxisomal function for biotechnological purposes. It aims to draw together the current state of the art as a convenient starting point for anyone, student or researcher, who wishes to know about plant peroxisomes.




The Pentose Phosphate Pathway


Book Description

The Pentose Phosphate Pathway aims to explore the pentose phosphate cycle and the practical techniques applied in its investigation. The main focus of the book is the pentose phosphate cycle in animals as well as microorganisms, and does not discuss the one related to photosynthesis. The book covers the formulation of the pathway, its types, and its alternative formulations; the preparation, processes, and analysis of the pathway; and the enzymes involved. Also covered in the book are the intermediates in intact cells and tissues; distribution of enzymes among different tissues and species; the operation, regulation, and overall control of the pathway; and the clinical, nutritional, and metabolic aspects of the pathway. The text is recommended for biologists and biochemists who would like to understand further the pentose phosphate pathway and the processes related to it.







Genetics Abstracts


Book Description




Photosynthetic Nitrogen Assimilation and Associated Carbon and Respiratory Metabolism


Book Description

According to many textbooks, carbohydrates are the photosynthesis and mitochondrial respiration fluctuate in a circadian manner in almost every unique final products of plant photosynthesis. However, the photoautotrophic production of organic organism studied. In addition, external triggers and environmental influences necessitate precise and nitrogenous compounds may be just as old, in appropriate re-adjustment of relative flux rates, to evolutionary terms, as carbohydrate synthesis. In the algae and plants of today, the light-driven assimilation prevent excessive swings in energy/resource provision of nitrogen remains a key function, operating and use. This requires integrated control of the alongside and intermeshing with photosynthesis and expression and activity of numerous key enzymes in respiration. Photosynthetic production of reduced photosynthetic and respiratory pathways, in order to carbon and its reoxidation in respiration are necessary co-ordinate carbon partioning and nitrogen assim- ation. to produce both the energy and the carbon skeletons required for the incorporation of inorganic nitrogen This volume has two principal aims. The first is to into amino acids. Conversely, nitrogen assimilation provide a comprehensive account of the very latest developments in our understanding of how green is required to sustain the output of organic carbon cells reductively incorporate nitrate and ammonium and nitrogen. Together, the sugars and amino acids into the organic compounds required for growth.