› › Stable Isotope Probing As A Tool In Microbial Ecology

Stable Isotope Probing

Author : Marc G. Dumont

Publisher : Humana

Page : 247 pages

File Size : 17,8 MB

Release : 2020-08-13

Category : Science

ISBN : 9781493997237

Get eBook

Book Description

This book provides definitive methods to perform stable isotope probing (SIP) experiments, covering a wide spectrum of stable isotope techniques used in microbial ecology, such as methods to target and analyze labeled DNA, rRNA, mRNA, protein, and PLFA. Protocols to study stable isotope fractionation by microbial pathways, the analysis of labeled communities with Raman microscopy, Chip-SIM, as well as quantitative SIP (qSIP) and high-resolution SIP (HR-SIP) are also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stable Isotope Probing: Methods and Protocols provides readers with up-to-date protocols ranging from basic to the most sophisticated applications of SIP and will benefit anyone pursuing this exciting area of study.



Stable Isotope Probing and Related Technologies

Author : J. Colin Murrell

Publisher : American Society for Microbiology Press

Page : 508 pages

File Size : 33,72 MB

Release : 2010-12-10

Category : Science

ISBN : 1555815375

Get eBook

Book Description

The ideal starting point for investigating, developing, and implementing stable isotope technologies. • Guides researchers through basic, tested, and proven protocols including DNA, RNA, protein, and phospholipid fatty acid (PLFA) SIP, from concept and history through detailed methodology, troubleshooting, and interpretation to optimal and future uses. • Explores important and emerging applications of SIP in environmental microbiology, ranging from bioremediation and gene mining to carbon tracking and gut microflora function. • Examines explorations of further elegant isotope labeling technologies such as Raman-FISH, NanoSIMS, and isotope arrays. • Serves as a valuable resource for environmental microbiology students and researchers and genomics, biotechnology, and medical microbiology professionals.



Methane and Methanol Utilizers

Author : J. Colin Murrell

Publisher : Springer

Page : 286 pages

File Size : 48,68 MB

Release : 1992-04-30

Category : Science

ISBN : 030643878X

Get eBook

Book Description

Methane and its oxidation product, methanol, have occupied an important position in the chemical industry for many years: the former as a feedstock, the latter as a primary chemical from which many products are produced. More recently, the role played by methane as a potent "greenhouse" gas has aroused considerable attention from environmentalists and clima tologists alike. This role for C compounds has, of course, been quite 1 incidental to the myriad of microorganisms on this planet that have adapted their life-styles to take advantage of these readily available am bient sources. Methane, a renewable energy source that will always be with us, is actually a difficult molecule to activate; so any microorganism that can effect this may point the way to catalytic chemists looking for con trollable methane oxidation. Methanol, formed as a breakdown product of plant material, is also ubiquitous and has also encouraged the growth of prokaryotes and eukaryotes alike. In an attempt to give a balanced view of how microorganisms have been able to exploit these simple carbon sources, we have asked a number ofleading scientists (modesty forbids our own inclusion here) to contribute chapters on their specialist areas of the subject.



A Primer on Stable Isotopes in Ecology

Author : Francesca Cotrufo

Publisher : Oxford University Press

Page : 129 pages

File Size : 29,40 MB

Release : 2023-09-05

Category : Stable isotopes in ecological research

ISBN : 0198854498

Get eBook

Book Description

In the past few decades, the field of ecology has made huge advancements thanks to stable isotopes. Ecologists need to understand the principles of stable isotopes to fully appreciate many studies in their discipline. Ecologists also need to be aware of isotopic approaches to enrich their "toolbox" for further advancing the discipline. A Primer on Stable Isotopes in Ecology is a concise and foundational resource for anyone interested in acquiring theoretical and practical knowledge for the application of stable isotopes in ecology. Readers will gain a more in-depth and complete knowledge of stable isotopes and explore isotopic methods used in ecological research, learning about stable isotope definitions, measurement, ecological processes, and applications in research. Chapters include in-depth descriptions of stable isotopes and their notation, isotope fractionation, isotope mixing, heavy isotope enrichment, and quantification methods by mass spectrometry and laser spectroscopy. The text guides readers to think "isotopically" to better understand research conducted using stable isotopes. The book also provides basic practical skills and activities to apply stable isotope methods in ecological research. It includes 5 activities through which readers can apply their knowledge to real-world problems and improve their skills for interpreting and using stable isotopes in ecological research. This book is designed for students and scientists from different backgrounds who share the common interest in stable isotopes.



Soil Microbiology, Ecology and Biochemistry

Author : Eldor Paul

Publisher : Academic Press

Page : 603 pages

File Size : 47,47 MB

Release : 2014-11-14

Category : Technology & Engineering

ISBN : 0123914116

Get eBook

Book Description

The fourth edition of Soil Microbiology, Ecology and Biochemistry updates this widely used reference as the study and understanding of soil biota, their function, and the dynamics of soil organic matter has been revolutionized by molecular and instrumental techniques, and information technology. Knowledge of soil microbiology, ecology and biochemistry is central to our understanding of organisms and their processes and interactions with their environment. In a time of great global change and increased emphasis on biodiversity and food security, soil microbiology and ecology has become an increasingly important topic. Revised by a group of world-renowned authors in many institutions and disciplines, this work relates the breakthroughs in knowledge in this important field to its history as well as future applications. The new edition provides readable, practical, impactful information for its many applied and fundamental disciplines. Professionals turn to this text as a reference for fundamental knowledge in their field or to inform management practices. - New section on "Methods in Studying Soil Organic Matter Formation and Nutrient Dynamics" to balance the two successful chapters on microbial and physiological methodology - Includes expanded information on soil interactions with organisms involved in human and plant disease - Improved readability and integration for an ever-widening audience in his field - Integrated concepts related to soil biota, diversity, and function allow readers in multiple disciplines to understand the complex soil biota and their function



Molecular Microbial Ecology

Author : Mark Osborn

Publisher : Garland Science

Page : 370 pages

File Size : 20,95 MB

Release : 2004-03-01

Category : Science

ISBN : 0203503392

Get eBook

Book Description

Microoganisms are distributed across every ecosystem, and microbial transformations are fundamental to the operation of the biosphere. Microbial ecology is the study of this interaction between microorganisms and their environment, and arguably represents one of the most important areas of biological research. Yet for many years our study of microbial flora was severely limited: the primary method of culturing microorganisms on media allowed us to study only between 0.1 and 10% of the total microbial flora in any given environment. Molecular Microbial Ecology gives a comprehensive guide to the recent revolution in the study of microorganisms in the environment. Details are given on molecular methods for isolating some of the previously uncultured and numerically dominant microbial groups. PCR-based approaches to studying prokaryotic systematics are described, including ribosomal RNA analysis and stable isotope probing. Later chapters cover DNA hybridisation techniques (including fluorescent in situ hybridisation), as well as genomic and metagenomic approaches to microbial ecology. Gathering together some of the world’s leading experts, this book provides an invaluable introduction to the modern theory and molecular methods used in studying microbial ecology.





Microbial Ecology of Activated Sludge

Author : Robert Seviour

Publisher : IWA Publishing

Page : 685 pages

File Size : 34,60 MB

Release : 2010-01-15

Category : Science

ISBN : 1843390329

Get eBook

Book Description

Microbial Ecology of Activated Sludge, written for both microbiologists and engineers, critically reviews our current understanding of the microbiology of activated sludge, the most commonly used process for treating both domestic and industrial wastes. The contributors are all internationally recognized as leading research workers in activated sludge microbiology, and all have made valuable contributions to our present understanding of the process. The book pays particular attention to how the application of molecular methods has changed our perceptions of the identity of the filamentous bacteria causing the operational disorders of bulking and foaming, and the bacteria responsible for nitrification and denitrification and phosphorus accumulation in nutrient removal processes. Special attention is given to how it is now becoming possible to relate the composition of the community of microbes present in activated sludge, and the in situ function of individual populations there, and how such information might be used to manage and control these systems better. Detailed descriptions of some of these molecular methods are provided to allow newcomers to this field of study an opportunity to apply them in their research. Comprehensive descriptions of organisms of interest and importance are also given, together with high quality photos of activated sludge microbes. Activated sludge processes have been used globally for nearly 100 years, and yet we still know very little of how they work. In the past 15 years the advent of molecular culture independent methods of study have provided tools enabling microbiologists to understand which organisms are present in activated sludge, and critically, what they might be doing there. Microbial Ecology of Activated Sludge will be the first book available to deal comprehensively with the very exciting new information from applying these methods, and their impact on how we now view microbiologically mediated processes taking place there. As such it will be essential reading for microbial ecologists, environmental biotechnologists and engineers involved in designing and managing these plants. It will also be suitable for postgraduate students working in this field.



Stable Isotope Ecology

Author : Brian Fry

Publisher : Springer Science & Business Media

Page : 318 pages

File Size : 25,51 MB

Release : 2007-01-15

Category : Science

ISBN : 0387337458

Get eBook

Book Description

A solid introduction to stable isotopes that can also be used as an instructive review for more experienced researchers and professionals. The book approaches the use of isotopes from the perspective of ecological and biological research, but its concepts can be applied within other disciplines. A novel, step-by-step spreadsheet modeling approach is also presented for circulating tracers in any ecological system, including any favorite system an ecologist might dream up while sitting at a computer. The author’s humorous and lighthearted style painlessly imparts the principles of isotope ecology. The online material contains color illustrations, spreadsheet models, technical appendices, and problems and answers.



Microbial Environmental Genomics (MEG)

Author : Francis Martin

Publisher : Springer Nature

Page : 370 pages

File Size : 10,11 MB

Release : 2022-12-15

Category : Science

ISBN : 1071628712

Get eBook

Book Description

This volume guides researchers on how to characterize, image rare, and hitherto unknown taxa and their interactions, to identify new functions and biomolecules and to understand how environmental changes condition the activity and the response of the organisms living with us and in our environment. Chapters cover different organism types (i.e., archaea, bacteria, fungi, protest, microfauna and microeukaryotes) and propose detailed protocols to produce high quality DNA, to analyse active microbial communities directly involved in complex interactions or processes through stable isotope probing, to identify and characterize of new functional genes, to image in situ interactions and to apply bioinformatics analysis tools to complex metagenomic or RNAseq sequence data. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Microbial Environmental Genomics (MEG): Methods and Protocols, Second Edition aims to serve as a primary research reference for researchers in microbiology working to in the expanding field of molecular ecology and environmental genomics.