› › Whole Genome Amplification Review Of Applications And Advances

Whole Genome Amplification

Author : Simon Hughes

Publisher : Methods Express (Paperback)

Page : 224 pages

File Size : 40,99 MB

Release : 2005

Category : Medical

ISBN :

Get eBook

Book Description

Whole genome amplification generates microgram quantities of genomic DNA starting from a sample of as little as a few femtograms and so is a vital technique when sample material is limited, as well as for high-throughput assays. Whole Genome Amplification: Methods Expressis a comprehensive up-to-date laboratory manual for this key technique. It provides detailed step-by-step protocols as well as hints and tips for success and troubleshooting, taking readers through all aspects of whole genome amplification. This book is an essential practical guide for any researcher currently using PCR for genomic amplification or who wishes to do so in future.



Clinical Applications for Next-Generation Sequencing

Author : Urszula Demkow

Publisher : Academic Press

Page : 336 pages

File Size : 33,27 MB

Release : 2015-09-10

Category : Science

ISBN : 0128018410

Get eBook

Book Description

Clinical Applications for Next Generation Sequencing provides readers with an outstanding postgraduate resource to learn about the translational use of NGS in clinical environments. Rooted in both medical genetics and clinical medicine, the book fills the gap between state-of-the-art technology and evidence-based practice, providing an educational opportunity for users to advance patient care by transferring NGS to the needs of real-world patients. The book builds an interface between genetic laboratory staff and clinical health workers to not only improve communication, but also strengthen cooperation. Users will find valuable tactics they can use to build a systematic framework for understanding the role of NGS testing in both common and rare diseases and conditions, from prenatal care, like chromosomal abnormalities, up to advanced age problems like dementia. - Fills the gap between state-of-the-art technology and evidence-based practice - Provides an educational opportunity which advances patient care through the transfer of NGS to real-world patient assessment - Promotes a practical tool that clinicians can apply directly to patient care - Includes a systematic framework for understanding the role of NGS testing in many common and rare diseases - Presents evidence regarding the important role of NGS in current diagnostic strategies



Advances in DNA Research and Application: 2012 Edition

Author :

Publisher : ScholarlyEditions

Page : 339 pages

File Size : 22,45 MB

Release : 2012-12-26

Category : Medical

ISBN : 1464994919

Get eBook

Book Description

Advances in DNA Research and Application / 2012 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about DNA. The editors have built Advances in DNA Research and Application / 2012 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about DNA in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Advances in DNA Research and Application / 2012 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.



DNA Amplification

Author : Vadim V. Demidov

Publisher : Taylor & Francis

Page : 342 pages

File Size : 47,11 MB

Release : 2004

Category : Science

ISBN : 9780954523299

Get eBook

Book Description

Whereas most books on DNA amplification focus on PCR-based technologies, this volume presents a wider range of methods to amplify DNA with an emphasis on their diverse applications. The book covers both well-established and newly-developed protocols including ligation-based thermocycling approaches, real-time PCR and other new PCR developments, plus several powerful non-PCR isothermal DNA amplification techniques, for example: real-time strand displacement amplification (SDA), rolling-circle amplification (RCA) and multiple-displacement amplification (MDA). An entire section is devoted to a group of enzymes, both natural and engineered, which are employed for DNA amplification and related purposes. In addition, the use of DNA amplification in the detection of non-DNA analytes is presented.



Precision Cancer Medicine

Author : Sameek Roychowdhury

Publisher : Springer Nature

Page : 196 pages

File Size : 15,9 MB

Release : 2020-01-02

Category : Medical

ISBN : 3030236374

Get eBook

Book Description

Genomic sequencing technologies have augmented the classification of cancer beyond tissue of origin and towards a molecular taxonomy of cancer. This has created opportunities to guide treatment decisions for individual patients with cancer based on their cancer’s unique molecular characteristics, also known as precision cancer medicine. The purpose of this text will be to describe the contribution and need for multiple disciplines working together to deliver precision cancer medicine. This entails a multi-disciplinary approach across fields including molecular pathology, computational biology, clinical oncology, cancer biology, drug development, genetics, immunology, and bioethics. Thus, we have outlined a current text on each of these fields as they work together to overcome various challenges and create opportunities to deliver precision cancer medicine. As trainees and junior faculty enter their respective fields, this text will provide a framework for understanding the role and responsibility for each specialist to contribute to this team science approach.



Dail and Hammar's Pulmonary Pathology

Author : Joseph F. Tomashefski

Publisher : Springer Science & Business Media

Page : 883 pages

File Size : 46,86 MB

Release : 2010-05-17

Category : Medical

ISBN : 0387721142

Get eBook

Book Description

Dail and Hammar’s Pulmonary Pathology has established itself as the definitive reference in the field. This third edition is now a two-volume, full color text. The new editorial board has continued to build upon the excellence previously achieved by reorganizing, expanding and substantially revising the text. This authoritative reference work has been updated to cover newly recognized entities and the latest advances in molecular diagnostic techniques. Abundantly illustrated with more than 2000 full color illustrations, this outstanding contribution to pathology literature is a must-have for the library of every surgical and pulmonary pathologist.



Next Generation Sequencing

Author : Jerzy Kulski

Publisher : BoD – Books on Demand

Page : 466 pages

File Size : 14,81 MB

Release : 2016-01-14

Category : Medical

ISBN : 9535122401

Get eBook

Book Description

Next generation sequencing (NGS) has surpassed the traditional Sanger sequencing method to become the main choice for large-scale, genome-wide sequencing studies with ultra-high-throughput production and a huge reduction in costs. The NGS technologies have had enormous impact on the studies of structural and functional genomics in all the life sciences. In this book, Next Generation Sequencing Advances, Applications and Challenges, the sixteen chapters written by experts cover various aspects of NGS including genomics, transcriptomics and methylomics, the sequencing platforms, and the bioinformatics challenges in processing and analysing huge amounts of sequencing data. Following an overview of the evolution of NGS in the brave new world of omics, the book examines the advances and challenges of NGS applications in basic and applied research on microorganisms, agricultural plants and humans. This book is of value to all who are interested in DNA sequencing and bioinformatics across all fields of the life sciences.



Advanced Topics in Forensic DNA Typing: Interpretation

Author : John M. Butler

Publisher : Academic Press

Page : 609 pages

File Size : 42,80 MB

Release : 2014-07-28

Category : Social Science

ISBN : 012405854X

Get eBook

Book Description

Advanced Topics in Forensic DNA Typing: Interpretation builds upon the previous two editions of John Butler's internationally acclaimed Forensic DNA Typing textbook with forensic DNA analysts as its primary audience. Intended as a third-edition companion to the Fundamentals of Forensic DNA Typing volume published in 2010 and Advanced Topics in Forensic DNA Typing: Methodology published in 2012, this book contains 16 chapters with 4 appendices providing up-to-date coverage of essential topics in this important field. Over 80 % of the content of this book is new compared to previous editions. - Provides forensic DNA analysts coverage of the crucial topic of DNA mixture interpretation and statistical analysis of DNA evidence - Worked mixture examples illustrate the impact of different statistical approaches for reporting results - Includes allele frequencies for 24 commonly used autosomal STR loci, the revised Quality Assurance Standards which went into effect September 2011



Human Genetics and Genomics

Author : Bruce R. Korf

Publisher : John Wiley & Sons

Page : 280 pages

File Size : 18,33 MB

Release : 2012-11-19

Category : Medical

ISBN : 1118537661

Get eBook

Book Description

This fourth edition of the best-selling textbook, Human Genetics and Genomics, clearly explains the key principles needed by medical and health sciences students, from the basis of molecular genetics, to clinical applications used in the treatment of both rare and common conditions. A newly expanded Part 1, Basic Principles of Human Genetics, focuses on introducing the reader to key concepts such as Mendelian principles, DNA replication and gene expression. Part 2, Genetics and Genomics in Medical Practice, uses case scenarios to help you engage with current genetic practice. Now featuring full-color diagrams, Human Genetics and Genomics has been rigorously updated to reflect today’s genetics teaching, and includes updated discussion of genetic risk assessment, “single gene” disorders and therapeutics. Key learning features include: Clinical snapshots to help relate science to practice 'Hot topics' boxes that focus on the latest developments in testing, assessment and treatment 'Ethical issues' boxes to prompt further thought and discussion on the implications of genetic developments 'Sources of information' boxes to assist with the practicalities of clinical research and information provision Self-assessment review questions in each chapter Accompanied by the Wiley E-Text digital edition (included in the price of the book), Human Genetics and Genomics is also fully supported by a suite of online resources at www.korfgenetics.com, including: Factsheets on 100 genetic disorders, ideal for study and exam preparation Interactive Multiple Choice Questions (MCQs) with feedback on all answers Links to online resources for further study Figures from the book available as PowerPoint slides, ideal for teaching purposes The perfect companion to the genetics component of both problem-based learning and integrated medical courses, Human Genetics and Genomics presents the ideal balance between the bio-molecular basis of genetics and clinical cases, and provides an invaluable overview for anyone wishing to engage with this fast-moving discipline.



Single Cell Diagnostics

Author : Alan R. Thornhill

Publisher : Springer Science & Business Media

Page : 185 pages

File Size : 43,89 MB

Release : 2008-02-02

Category : Science

ISBN : 159745298X

Get eBook

Book Description

This book applies modern molecular diagnostic techniques to the analysis of single cells, small numbers of cells, or cell extracts. Emphasis is placed on non-invasive analysis of single cell metabolites and the direct analysis of RNA and DNA from single cells, with a focus on polymerase chain reaction and fluorescence in situ hybridization. In particular, this handbook is essential for practitioners providing care for couples seeking treatment for infertility.