Plant Lipid Metabolism


Book Description

A collection of papers that comprehensively describe the major areas of research on lipid metabolism of plants. State-of-the-art knowledge about research on fatty acid and glycerolipid biosynthesis, isoprenoid metabolism, membrane structure and organization, lipid oxidation and degradation, lipids as intracellular and extracellular messengers, lipids and environment, oil seeds and gene technology is reviewed. The different topics covered show that modern tools of plant cellular and molecular biology, as well as molecular genetics, have been recently used to characterize several key enzymes of plant lipid metabolism (in particular, desaturases, thioesterases, fatty acid synthetase) and to isolate corresponding cDNAs and genomic clones, allowing the use of genetic engineering methods to modify the composition of membranes or storage lipids. These findings open fascinating perspectives, both for establishing the roles of lipids in membrane function and intracellular signalling and for adapting the composition of seed oil to the industrial needs. This book will be a good reference source for research scientists, advanced students and industrialists wishing to follow the considerable progress made in recent years on plant lipid metabolism and to envision the new opportunities offered by genetic engineering for the development of novel oil seeds.




Cell-wide Metabolic Alterations Associated with Malignancy


Book Description

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods providing a a theoretical overview on metabolic alterations of cancer cells and a series of protocols that can be employed to study oncometabolism, in vitro, ex vivo and in vivo. Malignant cells exhibit metabolic changes when compared to their normal counterparts, owing to both genetic and epigenetic alterations. Although such a metabolic rewiring has recently been indicated as "yet another" general hallmark of cancer, accumulating evidence suggests that the metabolic alterations of each neoplasm rather represent a molecular signature that intimately accompanies, and hence cannot be severed from, all facets of malignant transformation.




Plant Lipid Signaling Protocols


Book Description

As scientist begin to understand the complexity of lipid signaling and its roles in plant biology, there is an increasing interest in their analysis. Due to the low abundancy and transient nature of some of these hydrophobic compounds, this is not always easy. In Plant Lipid Signaling Protocols, expert researchers in the field detail experimental approaches by which plant signaling lipids can be studied. These methods and techniques include analysis of plant signaling lipids, including detailed protocols to detect various relevant compounds by targeted or non-targeted approaches; to assay relevant enzyme activities in biological material or using recombinant enzymes; to test for specific binding of signaling lipids to protein partners; or to visualize signaling lipids or lipid-derived signals in living plant cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Lipid Signaling Protocols aids plant researchers in the continuing to study the roles of lipid signals.




The Plant Plasma Membrane


Book Description

In plant cells, the plasma membrane is a highly elaborated structure that functions as the point of exchange with adjoining cells, cell walls and the external environment. Transactions at the plasma membrane include uptake of water and essential mineral nutrients, gas exchange, movement of metabolites, transport and perception of signaling molecules, and initial responses to external biota. Selective transporters control the rates and direction of small molecule movement across the membrane barrier and manipulate the turgor that maintains plant form and drives plant cell expansion. The plasma membrane provides an environment in which molecular and macromolecular interactions are enhanced by the clustering of proteins in oligimeric complexes for more efficient retention of biosynthetic intermediates, and by the anchoring of protein complexes to promote regulatory interactions. The coupling of signal perception at the membrane surface with intracellular second messengers also involves transduction across the plasma membrane. Finally, the generation and ordering of the external cell walls involves processes mediated at the plant cell surface by the plasma membrane. This volume is divided into three sections. The first section describes the basic mechanisms that regulate all plasma membrane functions. The second describes plasma membrane transport activity. The final section of the book describes signaling interactions at the plasma membrane. These topics are given a unique treatment in this volume, as the discussions are restricted to the plasma membrane itself as much as possible. A more complete knowledge of the plasma membrane’s structure and function is essential to current efforts to increase the sustainability of agricultural production of food, fiber, and fuel crops.




Computational Phytochemistry


Book Description

Computational Phytochemistry, Second Edition, explores how recent advances in computational techniques and methods have been embraced by phytochemical researchers to enhance many of their operations, refocusing and expanding the possibilities of phytochemical studies. By applying computational aids and mathematical models to extraction, isolation, structure determination, and bioactivity testing, researchers can obtain highly detailed information about phytochemicals and optimize working approaches. This book aims to support and encourage researchers currently working with or looking to incorporate computational methods into their phytochemical work. Topics in this book include computational methods for predicting medicinal properties, optimizing extraction, isolating plant secondary metabolites, and building dereplicated phytochemical libraries. The roles of high-throughput screening, spectral data for structural prediction, plant metabolomics, and biosynthesis are all reviewed before the application of computational aids for assessing bioactivities and virtual screening is discussed. Illustrated with detailed figures and supported by practical examples, this book is an indispensable guide for all those involved with the identification, extraction, and application of active agents from natural products. This new edition captures remarkable advancements in mathematical modeling and computational methods that have been incorporated in phytochemical research, addressing, e.g., extraction, isolation, structure determination, and bioactivity testing of phytochemicals. - Includes step-by-step protocols for various computational and mathematical approaches applied to phytochemical research - Features clearly illustrated chapters contributed by highly reputable researchers - Covers all key areas in phytochemical research, including virtual screening and metabolomics




Plant Salt Tolerance


Book Description

Soil salinity is destroying several hectares of arable land every minute. Because remedial land management cannot completely solve the problem, salt tolerant crops or plant species able to remove excessive salt from the soil could contribute significantly to managing the salinity problem. The key to engineering crops for salt tolerance lies in a thorough understanding of the physiological mechanisms underlying the adaptive responses of plants to salinity. Plant Salt Tolerance: Methods and Protocols describes recent advances and techniques employed by researchers to understand the molecular and ionic basis of salinity tolerance and to investigate the mechanisms of salt stress perception and signalling in plants. With chapters written by leading international scientists, this book covers nearly 30 different methods, such as microelectrode and molecular methods, imaging techniques, as well as various biochemical assays. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Plant Salt Tolerance: Methods and Protocols serves as an essential read for every student or researcher tackling various aspects of the salinity problem.




Plant Lipids


Book Description

New research tools have revealed many surprising aspects of thedynamic nature of lipids and their participation in processes suchas recognition, intra- and inter-cellular signalling, deterrenceand defence against pathogens, membrane trafficking and proteinfunction. This is in addition to new information on the moreestablished roles of plant lipids as structural components ofmembranes and as long-term storage products. Plant lipids are alsoincreasingly being seen as sources of a new generation ofenvironmentally friendly, biodegradable and renewable industrialproducts, including biopolymers and high grade lubricants. This volume provides a broad overview of plant lipid researchand its many applications, linking the various disciplines andproviding an interesting and wide-ranging perspective on thisfast-moving field. Extensive lists of references are provided,totalling well over two thousand non-redundant citations andoffering a point of entry to the detailed literature. This is a book for researchers and professionals in plantbiochemistry, molecular biology, biotechnology and genetics, inboth the academic and industrial sectors.




Recent Advances in Natural Products Analysis


Book Description

Recent Advances in Natural Products Analysis is a thorough guide to the latest analytical methods used for identifying and studying bioactive phytochemicals and other natural products. Chemical compounds, such as flavonoids, alkaloids, carotenoids and saponins are examined, highlighting the many techniques for studying their properties. Each chapter is devoted to a compound category, beginning with the underlying chemical properties of the main components followed by techniques of extraction, purification and fractionation, and then techniques of identification and quantification. Biological activities, possible interactions, levels found in plants, the effects of processing, and current and potential industrial applications are also included. - Focuses on the latest analytical techniques used for studying phytochemical and other biological compounds - Authored and edited by the top worldwide experts in their field - Discusses the current and potential applications and predicts future trends of each compound group




Lipids in Photosynthesis: Structure, Function and Genetics


Book Description

Lipids in Photosynthesis provides readers with a comprehensive view of the structure, function and genetics of lipids in plants, algae and bacteria, with special emphasis on the photosynthetic apparatus in thylakoid membranes. This volume includes the historical background of the field, as well as a full review of our current understanding of the structure and molecular organization of lipids and their role in the functions of photosynthetic membranes. The physical properties of membrane lipids in thylakoid membranes and their relationship to photosynthesis are also discussed. Other topics include the biosynthesis of glycerolipids and triglycerides; reconstitution of photosynthetic structures and activities with lipids; lipid-protein interactions in the import of proteins into chloroplasts; the development of thylakoid membranes as it relates to lipids; genetic engineering of the unsaturation of membrane glycerolipids, with a focus on the ability of the photosynthetic machinery to tolerate temperature stress; and the involvement of chloroplast lipids in the reactions of plants upon exposure to stress. This book is intended for a wide audience and should be of interest to advanced undergraduate and graduate students and to researchers active in the field, as well as to those scientists whose fields of specialization include the biochemistry, physiology, molecular biology, biophysics and biotechnology of membranes.




Lipids in Photosynthesis


Book Description

Lipids in Photosynthesis: Essential and Regulatory Functions, provides an essential summary of an exciting decade of research on relationships between lipids and photosynthesis. The book brings together extensively cross-referenced and peer-reviewed chapters by prominent researchers. The topics covered include the structure, molecular organization and biosynthesis of fatty acids, glycerolipids and nonglycerolipids in plants, algae, lichens, mosses, and cyanobacteria, as well as in chloroplasts and mitochondria. Several chapters deal with the manipulation of the extent of unsaturation of fatty acids and the effects of such manipulation on photosynthesis and responses to various forms of stress. The final chapters focus on lipid trafficking, signaling and advanced analytical techniques. Ten years ago, Siegenthaler and Murata edited "Lipids in Photosynthesis: Structure, Function and Genetics," which became a classic in the field. "Lipids in Photosynthesis: Essential and Regulatory Functions," belongs, with its predecessor, in every plant and microbiological researcher's bookcase.