Holographic Microscopy of Phase Microscopic Objects


Book Description

This book presents a clear and comprehensive review of the current status of three-dimensional (3D) digital holographic imaging of phase microscopic objects, with insightful discussions on the positive and negative features of classical, electronic and holographic microscopy. The technical details and results of the restoration of the 3D shapes of red blood cells, bacteria, yeasts, thin films and other micro-objects are presented. The physical background of the method was substantiated by the authors in 1989 and in 1998, the very first digital holographic interference microscope was developed. Clear evidence of the pathological flattened shape of the erythrocytes relevant to different pathologies is given by detailed measurements on the 3D images. Based on the model of the erythrocyte as a liquid-filled, charged, and viscoelastic shell reinforced by skeleton, numerical computations of the equilibrium shapes of human erythrocytes are made, and the results are analyzed and compared to the 3D visualization data.




A Handbook of the Microscope and Microscopic Objects


Book Description

This work has been selected by scholars as being culturally important, and is part of the knowledge base of civilization as we know it. This work was reproduced from the original artifact, and remains as true to the original work as possible. Therefore, you will see the original copyright references, library stamps (as most of these works have been housed in our most important libraries around the world), and other notations in the work. This work is in the public domain in the United States of America, and possibly other nations. Within the United States, you may freely copy and distribute this work, as no entity (individual or corporate) has a copyright on the body of the work.As a reproduction of a historical artifact, this work may contain missing or blurred pages, poor pictures, errant marks, etc. Scholars believe, and we concur, that this work is important enough to be preserved, reproduced, and made generally available to the public. We appreciate your support of the preservation process, and thank you for being an important part of keeping this knowledge alive and relevant.




Deep Under Cover


Book Description

You're used to looking at things as you see them with your naked eyes but what if you can see the world at a microscopic scale? That's going to be quite an experience, isn't it? This exciting educational resource will introduce your child to the world of microscopes. Hopefully, this book will start the seeds of new learning brought about by curiosity. Read today!




Handbook of Microscopy


Book Description

Handbook of Microscopy is a manual that deals mainly with the basic instruments and techniques used in light microscopy and its biological applications. A large section is devoted to the study of organic matter in microfossils preserved in rocks, in view of its stratigraphic importance in mining and oil prospecting. This text is comprised of six chapters; the first of which introduces the reader to the basic principles as well as to the instruments and techniques used in light microscopy. This book also discusses the microscopes and electronic flashlights for photomicrography, along with the use of monochromatic light, stereological and physicochemical microanalysis, microanalysis by electron microscopy, and microdetermination of physical values. Attention then turns to staining and impregnation and methods of fixation, examination, cutting, and mounting. The remaining chapters focus on the microscopy of topological stains and non-specific cytological stains, with emphasis on special methods used in animal and plant histology and protistology and mycological methods in pathology. This book is written specifically for microscopists.




Light and Video Microscopy


Book Description

The purpose of this book is to provide the most comprehensive, easy-to-use, and informative guide on light microscopy. Light and Video Microscopy will prepare the reader for the accurate interpretation of an image and understanding of the living cell. With the presentation of geometrical optics, it will assist the reader in understanding image formation and light movement within the microscope. It also provides an explanation of the basic modes of light microscopy and the components of modern electronic imaging systems and guides the reader in determining the physicochemical information of living and developing cells, which influence interpretation. - Brings together mathematics, physics, and biology to provide a broad and deep understanding of the light microscope - Clearly develops all ideas from historical and logical foundations - Laboratory exercises included to assist the reader with practical applications - Microscope discussions include: bright field microscope, dark field microscope, oblique illumination, phase-contrast microscope, photomicrography, fluorescence microscope, polarization microscope, interference microscope, differential interference microscope, and modulation contrast microscope




Handbook of Biological Confocal Microscopy


Book Description

This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging. Coverage includes practical multiphoton, photodamage and phototoxicity, 3D FRET, 3D microscopy correlated with micro-MNR, CARS, second and third harmonic signals, ion imaging in 3D, scanning RAMAN, plant specimens, practical 3D microscopy and correlated optical tomography.




Exploring with the Microscope


Book Description

Discusses the history and development of the microscope and the unseen world which it has made available for our study.