Book Description
This book is a high-level introduction, as well as a reference work for experienced users, to ECD, ETD, EDD, NETD, UVPD, SID, and other advanced fragmentation methods.
Author : Frederik Lermyte
Publisher : Royal Society of Chemistry
Page : 359 pages
File Size : 19,91 MB
Release : 2020-12-15
Category : Science
ISBN : 1839161043
This book is a high-level introduction, as well as a reference work for experienced users, to ECD, ETD, EDD, NETD, UVPD, SID, and other advanced fragmentation methods.
Author : Richard M. Caprioli
Publisher : Springer Science & Business Media
Page : 589 pages
File Size : 47,35 MB
Release : 2012-12-06
Category : Science
ISBN : 9401151652
Many fundamental aspects of the methods used in mass spectrometry are here presented by outstanding scientists, with reference to very recent developments. The principles and applications of electrospray, ion spray and MALDI ionization technique are presented, together with optimised GC/MS interfacing systems and tools for quantitative analysis. A comprehensive treatment of modern instrumentation for mass analysis and detection is also included. The major part of the book deals with bioanalytical applications to peptides, proteins, oligonucleotides, polysaccharides, lipids and plant metabolites. Several papers are devoted to the evaluation of adduct formation between DNA and carcinogens. Environmental applications are also included, with examples of some specific cases. Fundamentals and applications are treated with the same degree of depth: the first two parts of the book therefore provide a basis for the understanding of the biomolecular applications section. Audience: Ideal for advanced graduate students of chemistry who have learned some basic mass spectrometry. Also useful for Ph.D. students in chemistry, biology and medicine. Of value to researchers in academic and industrial laboratories.
Author : Hong Ji
Publisher :
Page : 158 pages
File Size : 38,5 MB
Release : 2007
Category : Biomolecules
ISBN : 9780549284581
In the last chapter I present our new discovery that FAB mass spectrometry in the negative ion mode can be used to unambiguously distinguish between cis- and trans- isomers of monounsaturated fatty acids by the relative strengths of two signals in the mass spectrum. I show the results of six fatty acids and demonstrate that under normal FAB ionization/desorption conditions, the deprotonated molecules [M-H]- of fatty acids undergo CRF. A characteristic fragmentation pattern of two intense clusters of mass peaks with three weak intervening peak-clusters can be used in each case to identify the position of the double bond. I also discuss the possibility of REC occurring during the FAB process.
Author : A. L. Burlingame
Publisher : Springer Science & Business Media
Page : 569 pages
File Size : 24,77 MB
Release : 2012-12-06
Category : Science
ISBN : 1461202299
Leading practitioners describe in detail advanced methods of mass spectrometry used in structural characterization of biomacromolecules of both natural and recombinant origin. They demonstrate by example how these methodologies can solve a wide array of real-world problems in protein biochemistry, immunology, and glycobiology, as well as for human bacterial pathogens, lipids, and nucleic acids. The book offers a unique opportunity to learn these techniques that are revolutionizing the field. Its authoritative assessment in the context of how to solve important and challenging problems in bioscience and medicine ensures a competitive advantage for today's researchers.
Author : Oscar Alzate
Publisher : CRC Press
Page : 356 pages
File Size : 36,84 MB
Release : 2009-10-26
Category : Medical
ISBN : 1420076264
In this, the post-genomic age, our knowledge of biological systems continues to expand and progress. As the research becomes more focused, so too does the data. Genomic research progresses to proteomics and brings us to a deeper understanding of the behavior and function of protein clusters. And now proteomics gives way to neuroproteomics as we beg
Author : Urs P. Schlunegger
Publisher : Elsevier
Page : 156 pages
File Size : 12,56 MB
Release : 2016-01-22
Category : Science
ISBN : 1483188876
Advanced Mass Spectrometry: Applications in Organic and Analytical Chemistry discusses the concepts that are essential in the effective utilization of mass spectrometry. The title particularly covers the fundamentals of the modern techniques, along with the technological concerns of mass spectrometry. The opening chapter of the selection introduces mass spectrometry, while the subsequent chapters cover the fundamentals and hardware. The next chapters talk about the analytical chemistry consequences and the ion-genetic relationships. The remaining chapter covers the application of mass spectrometry, which includes structural, mechanistic, chemical, and biochemical applications. The book will be of great use to organic and analytical chemists. Chemists from other branch of chemistry, along with practitioners of related fields such as chemical engineering will also benefit from the text.
Author : Richard M. Caprioli
Publisher : Springer Science & Business Media
Page : 531 pages
File Size : 43,85 MB
Release : 2012-12-06
Category : Science
ISBN : 9400902174
Mass Spectrometry in the Biological Sciences covers the most recent technological and applied developments in the area, including both ionization techniques and ion analysis. It introduces and reviews some of the newer ionization methods, describes the major instrumentation involved in mass analysis, and presents the scope of the technology in biology, medicine, and environmental science. Specific examples are given for a number of topics. It also deals with recent achievements in the on-line combination of separation techniques such as gas chromatography, liquid chromatography, and supercritical fluid technology.
Author : M.L Gross
Publisher : Springer Science & Business Media
Page : 462 pages
File Size : 24,77 MB
Release : 2012-12-06
Category : Science
ISBN : 9401126186
The developments in mass spectrometry over the past fifteen years have been impressive in their implications in bioanalytical chemistry. The achievements begin with the inventions of Cf-252 Plasma Desorption Mass Spectrometry by Macfarlane and Fourier Transform Mass Spectrometry by Comisarow and Marshall in the mid 1970s. The former showed the feasibility of producing large gas-phase ions from large biomolecules whereas the latter enhanced the capabilities for ion trapping especially in analytical mass spectrometry. A major achievement was the development by Barber of Fast Atom Bombardment (FAB) mass spectrometry, an advance that heralded a new era in biological mass spectrometry. Contemporary and routine instruments such as magnetic sectors and quadrupoles were rapidly adapted to F AB, and nearly the entire universe of small molecules became amenable to study by mass spectrometry. The introduction of FAB also paved the way for improvement of instrument capability. For example, the upper mass limit of magnet sector mass spectrometers was increased by nearly an order of magnitude by the instrument manufacturers. Furthermore, the technique of tandem mass spectrometry (MS/MS) was given new meaning because important structural information for biomolecules could now be produced for ions introduced by FAB into the tandem instrument. The evolution of MS/MS continues today with the development of ion traps, time-of-flight, and sector instruments equipped with array detection.
Author : Rawi Ramautar
Publisher : Royal Society of Chemistry
Page : 277 pages
File Size : 15,60 MB
Release : 2021-07-13
Category : Science
ISBN : 1839163534
The efficient analysis of polar and charged metabolites in biological samples remains a huge challenge in the field of metabolomics. Over the past years, novel mass spectrometry-based analytical tools have been developed to enable the sensitive and efficient profiling of polar ionogenic metabolites in various biological samples. This book gives the reader a comprehensive overview of these recent technological developments. Topics covered include the use of chemical labelling strategies for allowing the analysis of polar metabolites using reversed-phase liquid chromatography–mass spectrometry (RPLC-MS) and the latest methodological developments in RPLC-MS, hydrophilic interaction liquid chromatography (HILIC)-MS and ion-pair LC-MS approaches. Attention is also paid to developments in nano-LC-MS and capillary electrophoresis–mass spectrometry methods specifically for profiling polar metabolites in small volume biological samples. The utility of ion-mobility MS and NMR spectroscopy will also be outlined. Sample preparation is the key part in the analytical workflow employed for metabolomics. Therefore, ample emphasis will be given on recent solid-phase extraction and solid-phase micro-extraction methods. Finally, analytical techniques for chiral metabolic profiling will also be considered. Discussing the state-of-the-art of the proposed topics in one single book for probing the polar metabolome, using relevant examples, is unique and needed in the metabolomics field. This book has relevance and appeal to an international audience of analytical and biomedical researchers in industry and academia.
Author : Chava Lifshitz
Publisher : Wiley-Interscience
Page : 0 pages
File Size : 10,50 MB
Release : 2006-10-06
Category : Science
ISBN : 9780471721840
An extensive compilation of articles by leading professionals, this reference explains the fundamental principles of mass spectrometry as they relate to the life sciences. Topics covered include spectroscopy, energetics and mechanisms of peptide fragmentation, electron capture dissociation, ion-ion and ion-molecule reactions, reaction dynamics, collisional activation, soft-landing, protein structure and interactions, thermochemistry, and more. The book empowers readers to develop new ways of using these techniques.