› › Analysis Of Carbohydrates In Food And Glycoconjugates With Capillary Electrophoresis

Carbohydrate Analysis by Modern Liquid Phase Separation Techniques

Author : Ziad El Rassi

Publisher : Elsevier

Page : 956 pages

File Size : 14,36 MB

Release : 2021-08-15

Category : Science

ISBN : 0128245123

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Book Description

Carbohydrate Analysis by Modern Liquid Phase Separation Techniques, Second Edition, presents readers with the various principles of modern liquid phase separation techniques and their contributions to the analysis of complex carbohydrates and glycoconjugates. In a selection of all-new chapters, this fully updated volume covers each technique in detail. The book aims to help analysts solve any of the many practical problems they may face in tackling the analysis of carbohydrates. In addition, it addresses current difficulties that must be resolved in carbohydrate research, thus inspiring further important technological developments to meet these challenges. This is an essential resource for anyone seeking a broad view of the science of carbohydrates and separation techniques. - Covers the basic principles of modern liquid phase separation techniques, along with their applications - Compiles up-to-date information on the field of carbohydrate analysis, along with updates on separation science - Focuses on problems currently faced in carbohydrate analysis and the solutions necessary for further progress



Carbohydrate Analysis

Author : Z. El Rassi

Publisher : Elsevier

Page : 693 pages

File Size : 21,75 MB

Release : 1994-11-11

Category : Science

ISBN : 008085866X

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Book Description

Carbohydrates and glycoconjugates play an important role in several life processes. The wide variety of carbohydrate species and their inherent polydispersity and heterogeneity require separation techniques of high resolving power and high selectivity such as high performance liquid chromatography (HPLC) and capillary electrophoresis (HPCE). In the last decade HPLC, and recently HPCE methods have been developed for the high resolution and reproducible quantitation of carbohydrates. Despite the importance of these two column separation technologies in the area of carbohydrates, no previous book describes specialized methods for the separation, purification and detection of carbohydrates and glycoconjugates by HPLC and HPCE.Therefore, the objective of the present book is to provide a comprehensive review of carbohydrate analysis by HPLC and HPCE by covering analytical and preparative separation techniques for all classes of carbohydrates including mono- and disaccharides; linear and cyclic oligosaccharides; branched heterooligosaccharides (e.g., glycans, plant-derived oligosaccharides); glycoconjugates (e.g., glycolipids, glycoproteins); carbohydrates in food and beverage; compositional carbohydrates of polysaccharides; carbohydrates in biomass degradation; etc.The book will be of interest to a wide audience, including analytical chemists and biochemists, carbohydrate, glycoprotein and glycolipid chemists, molecular biologists, biotechnologists, etc. It will also be a useful reference work for both the experienced analyst and the newcomer as well as for users of HPLC and HPCE, graduates and postdoctoral students.



Capillary Electrophoresis of Carbohydrates

Author : Nicola Volpi

Publisher : Springer Science & Business Media

Page : 286 pages

File Size : 32,47 MB

Release : 2010-10-26

Category : Science

ISBN : 1607618753

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Book Description

Simple carbohydrates, complex oligosaccharides and polysaccharides all belong to a class of ubiquitous (macro)molecules that exhibit a wide range of biological functions, and the recent advent of enhanced enzymatic, chemical and analytical tools used to study these sugars has inaugurated a genuine explosion in the field of glycomics. Specifically, it has led to a deeper understanding of how specific sugar structures modulate cellular phenotypes, and that breakthrough has led to the discovery of new pharmaceuticals for the treatment of many serious diseases, such as cancer. The subsequent rapid expansion of this research holds high promise for future therapeutic regimens, and capillary electrophoresis (CE) refers to the range of related separation techniques that are integral to this vital research. CE uses narrow-bore fused-silica capillaries to separate a complex array of large and small molecules, and Capillary Electrophoresis of Carbohydrates offers a comprehensive look at the latest breakthroughs and improvements in CE and CE techniques applied to monosaccharides up to complex oligosaccharides and polysaccharides. It begins with an overview of the application of CE and CE- mass spectrometric in the analysis of simple carbohydrates without any previous derivatization step before discussing various detection techniques such as spectrophotometric detection, electrochemical detection and other less common techniques. It then covers in detail an array of related topics and numerous applications. It is an essential text for anyone exploring the myriad possibilities of this rapidly expanding field.



Carbohydrate Analysis by Modern Chromatography and Electrophoresis

Author : Ziad El Rassi

Publisher : Elsevier

Page : 1255 pages

File Size : 11,43 MB

Release : 2002-10-31

Category : Science

ISBN : 008052849X

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Book Description

This book is an updated and expanded edition of Carbohydrate Analysis, High Performance Liquid Chromatography and Capillary Electrophoresis and is concerned with the analysis of carbohydrates by modern chromatography and electrophoresis including analytical and preparative high performance liquid chromatography (HPLC), thin layer chromatography (TLC), field flow fractionation (FFF), capillary electrophoresis (CE), capillary electrochromatography (CEC), polyacrylamide gel electrophoresis (PAGE), gas chromatography (GC) and supercritical fluid chromatography (SFC). Thirty-one chapters cover: various modes of HPLC, CE, CEC, FFF, GC and SFC that are currently applied to the analysis of carbohydrates; discussions on analytical and preparative separations; descriptions of the principles of detection and quantitative determination of carbohydrates by the various separation techniques; reviews of sample preparations; and information on important applications. Furthermore, the book describes in detail the different direct and indirect detection methods that have been introduced for the sensitive detection of carbohydrates. This title is useful for a wide audience including separation scientists; analytical chemists and biochemists; carbohydrate chemists; glycoprotein and glycolipid chemists; molecular biologists; and biotechnologists. The book is also a useful reference for both the experienced analyst and the newcomer and for users of modern chromatography and electrophoresis.·Contains 31 chapters covering all aspects of carbohydrate analysis by modern chromatography and electrophoresis·Each chapter discusses the basic principles, advantages and limitations, and applications of the particular detection technique·Useful reference for both the experienced analyst and the newcomer





Capillary Gel Electrophoresis

Author : Andras Guttman

Publisher : Newnes

Page : 391 pages

File Size : 32,83 MB

Release : 2021-12-04

Category : Science

ISBN : 0080931359

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Book Description

Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. - Covers all theoretical and practical aspects of capillary gel electrophoresis - Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods - Teaches readers how to use the technology and select methods that are ideal for fundamental problems - Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses



Food Analysis

Author : Suzanne Nielsen

Publisher : Springer Science & Business Media

Page : 584 pages

File Size : 14,10 MB

Release : 2003-04-30

Category : Technology & Engineering

ISBN : 9780306474958

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Book Description

This book provides information on the techniques needed to analyze foods in laboratory experiments. All topics covered include information on the basic principles, procedures, advantages, limitations, and applications. This book is ideal for undergraduate courses in food analysis and is also an invaluable reference to professionals in the food industry. General information is provided on regulations, standards, labeling, sampling and data handling as background for chapters on specific methods to determine the chemical composition and characteristics of foods. Large, expanded sections on spectroscopy and chromatography are also included. Other methods and instrumentation such as thermal analysis, selective electrodes, enzymes, and immunoassays are covered from the perspective of their use in the chemical analysis of foods. A helpful Instructor's Manual is available to adopting professors.



Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques

Author : James P. Landers

Publisher : CRC Press

Page : 1598 pages

File Size : 33,12 MB

Release : 2007-12-18

Category : Medical

ISBN : 1420004956

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Book Description

Now in its third edition, this bestselling work continues to offer state-of-the-art information on the development and employment of capillary electrophoresis. With special emphasis on microseparations and microfluidics, it features new chapters describing the use of microchip electrophoresis and associated microtechniques, with a focus on the extraordinary breadth of work undertaken to expand CE methodologies in recent years. Enhanced by contributions from leading international experts, the Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition remains a seminal reference for the chemistry, biology, and engineering fields.



Methods of Analysis of Food Components and Additives

Author : Semih Otles

Publisher : CRC Press

Page : 531 pages

File Size : 17,19 MB

Release : 2011-11-16

Category : Science

ISBN : 1439815534

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Book Description

With diet, health, and food safety news making headlines on a regular basis, the ability to separate, identify, and analyze the nutrients, additives, and toxicological compounds found in food and food components is more important than ever. This requires proper training in the application of best methods, as well as efforts to improve existing meth



Carbohydrate Analysis by Modern Chromatography and Electrophoresis

Author : Ziad El Rassi

Publisher : Elsevier

Page : 1270 pages

File Size : 37,18 MB

Release : 2002-10-31

Category : Language Arts & Disciplines

ISBN :

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Book Description

Selected Papers. The Solute; Modern Liquid Chromatography: Fundamentals, Methodologies and Applications; Preparative Liquid Chromatography; Field Flow Fractionation; Electrophoresis and Electrochromatography: Fundamentals, Methodologies and Applications; Gas Chromatography; The Detection.