Author : American Medical Association
Publisher : Forgotten Books
Page : 236 pages
File Size : 14,85 MB
Release : 2018-02-21
Category : Medical
ISBN : 9780666057709
Book Description
Excerpt from Annual Report (Reprinted Papers) Of the Investigations Carried Out Under the Supervision of the Therapeutic Research Committee of the Council on Pharmacy and Chemistry of the American Medical Association, 1917, Vol. 6 The trypsin test was made on a larger aliquot part Of the stomach contents and in this manner: Twenty five C.c. (occasionally less) of the liquid was made neutral to phenolphthalein with Sodium hydroxide and mixed with 20 cc. Of a molar/15 phosphate solution having a PH value Of A gram of powdered fibrin was added and Shaken until soaked and swollen, after which the mixture was made to 50 cc. And incubated through three hours at A parallel blank test was always made on an equal volume of the stomach con tents, but boiled to kill the ferment present. After the incubation the 50 CC. Digested mixtures were chilled to stop action, poured into 100 cc. Cylinders containing sufficient volume Of barium hydroxide and chloride solution to precipitate all the phosphate pres ent, and made up to the mark with water. The cylin ders were allowed to stand overnight to permit the precipitates to settle, after which 50 cc portions were poured or filtered off, neutralized to phenolphthalein and titrated with N alkali in presence of formal dehyde in the usual manner. From the results Of the titrations in the unboiled samples the blank results were subtracted. The differences multiplied by 2 gave the titration values for the original portions (25 pc.c. Or Ies's), measured from the stomach contents. The values for the whole contents were, in turn, calculated from these. In Table A the data, as so outlined, are given. About the Publisher Forgotten Books publishes hundreds of thousands of rare and classic books. Find more at www.forgottenbooks.com This book is a reproduction of an important historical work. Forgotten Books uses state-of-the-art technology to digitally reconstruct the work, preserving the original format whilst repairing imperfections present in the aged copy. In rare cases, an imperfection in the original, such as a blemish or missing page, may be replicated in our edition. We do, however, repair the vast majority of imperfections successfully; any imperfections that remain are intentionally left to preserve the state of such historical works.