Application of Some Proteome Analysis Techniques in Animal Reproduction


Book Description

This chapter focuses on the application of proteome analysis techniques to animal reproduction and provides general information on one-dimensional (1D) and two-dimensional (2D) electrophoresis, chromatographic methods, and mass spectrometer (MS), widely used in proteomics studies. Proteome consists of an entire complement of proteins expressed by a cell, tissue, or organism. Proteomics reveals functions of proteins encoded in the genome. These functions include posttranslational modifications (PTMs) and alterations in the protein synthesis. Animal reproduction takes a key role in livestock, and increasing the reproduction rate in flocks plays an important role in livestock management. Studying the proteins related to reproduction could guide on how to increase fertility. Recent studies addressed the proteome constitution of both male and female reproductive system. Follicular fluid, endometrium, and ovary proteins were analyzed in females by proteomics study, while in males, sperm proteomics was more focused. Information obtained on this issue is also beneficial for the development of reproductive technologies such as in vitro fertilization and embryo transfer. Strategies to increase fertility in animals can be revealed by proteomic studies, and a more profound knowledge on proteomics may become helpful to develop and enhance the efficiency of reproductive technologies.




Methods in Animal Proteomics


Book Description

The study of proteomics provides researchers with a better understanding of disease and physiological processes in animals. Methods in Animal Proteomics will provide animal scientists and veterinarians currently researching these topics in domestic animals a firm foundation in the basics of proteomics methodology, while also reviewing important advances that will be of interest to established researchers in the field. Chapters will provide practical information on a range of topics including protein identification and separation, bioinformatics, and applications to disease and reproduction research. This text will be written by leading international proteomics experts and essential for researchers in the fields of animal biology and veterinary medicine.




Proteomics in Domestic Animals: from Farm to Systems Biology


Book Description

Proteomics, like other post-genomics tools, has been growing at a rapid pace and has important applications in numerous fields of science. While its use in animal and veterinary sciences is still limited, there have been considerable advances in this field in recent years, in areas as diverse as physiology, nutrition and food of animal origin processing. This is mainly as a consequence of a wider availability and better understanding of proteomics methodologies by animal and veterinary researchers. This book provides a comprehensive, state-of-the-art account of the status of farm-animal proteomics research, focusing on the principles behind proteomics methodologies and its specific applications and offering clear example.




Farm animal proteomics


Book Description

Proteomics is a field with growing interest in almost every area of life sciences. Proteomic techniques, along with potential and obtained results have long been key components of biomedical and pharmaceutical research. In agriculture, animal and veterinary research however, use of proteomics is still limited, despite the large number of potential applications. As a result, there is a pressing need for wider use and dissemination of proteomics in animal and veterinary science research. This book meets this need by bridging the gap between experts in the technology of proteomics and those at the forefront of research into many facets of animal physiology and pathophysiology. The book encompasses a wide range of topics: from muscle and meat proteomics to acute phase proteins and proteomics in aquaculture, to name but a few. The book summarily presents the state of the art in farm animal proteomics research in Europe, providing readers with interesting examples of the applications of this set of advanced technologies as well as useful contact details of colleagues with expertise in the field. The book will be of interest to scientists at the cutting edge of animal and veterinary research who are currently, or are considering, analytical investigation and identification of protein in animal tissue and in pathogens in health and disease. Furthermore the book will provide an insight for proteomic specialists in demonstrating the breadth of applications that their technology can have in animal and food research.




Quantitative Proteome Analysis


Book Description

This book focuses on the advantages and disadvantages of each of the commonly used quantitative proteomic methods in terms of accuracy, sensitivity, and reproducibility. It also concentrates on the effective applications of these methods that resulted in many discoveries of the role of the proteins expressed in living cells and biological fluids. The first part of the book focuses on the description of advantages and disadvantages of each of the commonly used quantitative proteomic methods in terms of accuracy, sensitivity, and, especially, reproducibility. The second part of the book focuses on providing concise descriptions of the effective applications of these methods to demonstrate how they have resulted in many important discoveries of the roles of the proteins expressed in living cells.




Agricultural Proteomics Volume 1


Book Description

This book will cover several topics to elaborate how proteomics may enhance agricultural productivity. These include crop and food proteomics, farm animal proteomics, aquaculture, microorganisms and insect proteomics. It will also cover several technical advances, which may address the current need for comprehensive proteome analysis.An emerging field of the proteomics aim is to integrate knowledge from basic sciences and to translate it into agricultural applications to solve issues related to economic values of farm animals, crops, food security, health, and energy sustainability. Given the wealth of information generated and to some extent applied in agriculture, there is the need for more efficient and broader channels to freely disseminate the information to the scientific community.




Current and Future Reproductive Technologies and World Food Production


Book Description

​​​​​​​​​​​This book addresses the impacts of current and future reproductive technologies on our world food production and provides a significant contribution to the importance of research in the area of reproductive physiology that has never been compiled before. It would provide a unique opportunity to separate the impacts of how reproductive technologies have affected different species and their contributions to food production. Lastly, no publication has been compiled that demonstrates the relationship between developments in reproductive management tools and food production that may be used a reference for scientists in addressing future research areas. ​During the past 50 years assisted reproductive technologies have been developed and refined to increase the number and quality of offspring from genetically superior farm animal livestock species. Artificial insemination (AI), estrous synchronization and fixed-time AI, semen and embryo cryopreservation, multiple ovulation and embryo transfer (MOET), in vitro fertilization, sex determination of sperm or embryos, and nuclear transfer are technologies that are used to enhance the production efficiency of livestock species.




New Insights into Theriogenology


Book Description

This book covers a variety of topics on animal reproduction and reproductive medicine. With evolving technology and a continual increase in knowledge, regarding domestic pets or agricultural animals, new information is available on diverse topics in this broad field. The book contents reflect the individual experience of authors, who developed a number of themes identified as attracting interest in the field. As it is, new opportunities were opened for productive collaboration. We have tried to provide you with current, specialised information that may be useful to students, clinicians and researchers. We hope this book inspires you to embrace these themes, foster the debate on particular topics and may be used as a start-up source for exploring the theriogenology field.




Biology for AP ® Courses


Book Description

Biology for AP® courses covers the scope and sequence requirements of a typical two-semester Advanced Placement® biology course. The text provides comprehensive coverage of foundational research and core biology concepts through an evolutionary lens. Biology for AP® Courses was designed to meet and exceed the requirements of the College Board’s AP® Biology framework while allowing significant flexibility for instructors. Each section of the book includes an introduction based on the AP® curriculum and includes rich features that engage students in scientific practice and AP® test preparation; it also highlights careers and research opportunities in biological sciences.




Proteomics for Food Authentication


Book Description

Consumers have the right to know what is in the food they are eating, and accordingly, a number of global food regulations require that the provenance of the food can be guaranteed from farm to fork. Many different instrumental techniques have been proposed for food authentication. Although traditional methods are still being used, new approaches such as genomics, proteomics, and metabolomics are helping to complement existing methodologies for verifying the claims made about certain food products. During the last decade, proteomics (the largescale analysis of proteins in a particular biological system at a particular time) has been applied to different research areas within food technology. Since proteins can be used as markers for many properties of a food, even indicating processes to which the food has been subjected, they can provide further evidence of the foods labeling claim. Proteomics for Food Authentication, a volume in the Food Analysis and Properties Series, is a comprehensive and updated overview of the applications, drawbacks, advantages, and challenges of proteomics for food authentication. Features: Provides a comprehensive and critical overview of the application of proteomics in food Helps food scientists determine the authenticity of several food products Provides applied techniques for both laboratory and industrial environments Describes workflows, technologies, and tools that are being assessed in proteomics-related studies Workflows, technologies, and tools that are being assessed in proteomics-related studies are described, followed by a review of the specific applications regarding food authenticity and, now and then, food quality. The book will provide a comprehensive and critical overview of the application of proteomics approaches to determine the authenticity of several food products updating the performances and current limitations of the applied techniques in both laboratory and industrial environments. As such it is well suited to food scientist, chemical engineers, food engineers, research labs, universities, governments, related food industries. Also available in the Food Analysis and Properties Series: Food Aroma Evolution: During Food Processing, Cooking, and Aging, edited by Matteo Bordiga and Leo M.L. Nollet (ISBN: 9781138338241) Ambient Mass Spectroscopy Techniques in Food and the Environment, edited by Leo M.L. Nollet and Basil K. Munjanja (ISBN: 9781138505568) Hyperspectral Imaging Analysis and Applications for Food Quality, edited by N.C. Basantia, Leo M.L. Nollet, and Mohammed Kamruzzaman (ISBN: 9781138630796) For a complete list of books in this series, please visit our website at: www.crcpress.com/Food-Analysis--Properties/book-series/CRCFOODANPRO