Book Description
Hybridized DNA can be visualized as an intense bluish-purple band.By using this BluGene non-radioactive probe, we were able to map the cI gene on the lambda DNA genome. Results and the application of the system will be presented and discussed. The second system is PhotoGene Nucleic Acid Detection System. When the membrane is incubated with a substrate for alkaline phosphatase that luminesces when dephosphorylated, dark bands can be visualized by using a X-ray film. By using this method, we have labelled a yeast DNA fragment of 6.6 kb as a probe and hybridized with the restriction endonuclease digested yeast genomic DNA after Southeast transfer. DNA was visualized as dark bands in X-ray film. [Authors' abstract].