Biochemistry in the Lab


Book Description

Most lab manuals assume a high level of knowledge among biochemistry students, as well as a large amount of experience combining knowledge from separate scientific disciplines. Biochemistry in the Lab: A Manual for Undergraduates expects little more than basic chemistry. It explains procedures clearly, as well as giving a clear explanation of the theoretical reason for those steps. Key Features: Presents a comprehensive approach to modern biochemistry laboratory teaching, together with a complete experimental experience Includes chemical biology as its foundation, teaching readers experimental methods specific to the field Provides instructor experiments that are easy to prepare and execute, at comparatively low cost Supersedes existing, older texts with information that is adjusted to modern experimental biochemistry Is written by an expert in the field This textbook presents a foundational approach to modern biochemistry laboratory teaching together with a complete experimental experience, from protein purification and characterization to advanced analytical techniques. It has modules to help instructors present the techniques used in a time critical manner, as well as several modules to study protein chemistry, including gel techniques, enzymology, crystal growth, unfolding studies, and fluorescence. It proceeds from the simplest and most important techniques to the most difficult and specialized ones. It offers instructors experiments that are easy to prepare and execute, at comparatively low cost.




Fundamentals of Biochemical Calculations


Book Description

Fundamentals of Biochemical Calculations, Second Edition demystifies the fundamental calculations used in modern biochemistry, cell biology, and allied biomedical sciences. The book encouragesbothundergraduates and scientists to develop an understanding of the processes involved in performing biochemical calculations, rather than rely on mem




Biochemistry Laboratory


Book Description

The biochemistry laboratory course is an essential component in training students for careers in biochemistry, molecular biology, chemistry, and related molecular life sciences such as cell biology, neurosciences, and genetics. Increasingly, many biochemistry lab instructors opt to either design their own experiments or select them from major educational journals. Biochemistry Laboratory: Modern Theory and Techniques addresses this issue by providing a flexible alternative without experimental protocols. Instead of requiring instructors to use specific experiments, the book focuses on detailed descriptions of modern techniques in experimental biochemistry and discusses the theory behind such techniques in detail. An extensive range of techniques discussed includes Internet databases, chromatography, spectroscopy, and recombinant DNA techniques such as molecular cloning and PCR. The Second Edition introduces cutting-edge topics such as membrane-based chromatography, adds new exercises and problems throughout, and offers a completely updated Companion Website.




Computers in Chemical and Biochemical Research V1


Book Description

Computers in Chemical and Biochemical Research, Volume 1, is the first of multi-volume series that provides updated information on developments in computer-assisted techniques for chemical and biochemical research. Each volume will include a variety of review articles by expert practicing computer-chemists and biochemists, dealing with a wide array of topics. These will range from delineations of design philosophies and of laboratory computer systems to descriptions of special computer hardware developed to solve specific problems. Also included will be articles discussing algorithms, languages, and programming techniques; articles devoted to the more important applications of digital computer methods to specific problems (e.g., Fourier transform spectrometry, stopped-flow kinetics, automated spectrophotometry); and aticles devoted to techniques for training both graduates and undergraduates in laboratory computing. The present text contains seven chapters and begins with a description of an educational program on digital computer instrumentation for the chemistry laboratory. Subsequent chapters cover topics such as multiprocessor systems in laboratory automation; a simple computer system for on-line data collection and manipulation in the biochemical laboratory; Fourier transform spectroscopy; and a small computer system used to collect and process data from three electron spin resonance spectrometers at the University of Oregon.




Essential Numerical Computer Methods


Book Description

The use of computers and computational methods has become ubiquitous in biological and biomedical research. During the last 2 decades most basic algorithms have not changed, but what has is the huge increase in computer speed and ease of use, along with the corresponding orders of magnitude decrease in cost. A general perception exists that the only applications of computers and computer methods in biological and biomedical research are either basic statistical analysis or the searching of DNA sequence data bases. While these are important applications they only scratch the surface of the current and potential applications of computers and computer methods in biomedical research. The various chapters within this volume include a wide variety of applications that extend far beyond this limited perception. As part of the Reliable Lab Solutions series, Essential Numerical Computer Methods brings together chapters from volumes 210, 240, 321, 383, 384, 454, and 467 of Methods in Enzymology. These chapters provide a general progression from basic numerical methods to more specific biochemical and biomedical applications. - The various chapters within this volume include a wide variety of applications that extend far beyond this limited perception - As part of the Reliable Lab Solutions series, Essential Numerical Computer Methods brings together chapters from volumes 210, 240, 321, 383, 384, 454, and 467 of Methods in Enzymology - These chapters provide a general progression from basic numerical methods to more specific biochemical and biomedical applications




Fundamental Laboratory Approaches for Biochemistry and Biotechnology


Book Description

Ninfa/Ballou/Benore is a solid biochemistry lab manual, dedicated to developing research skills in students, allowing them to learn techniques and develop the organizational approaches necessary to conduct laboratory research. Ninfa/Ballou/Benore focuses on basic biochemistry laboratory techniques with a few molecular biology exercises, a reflection of most courses which concentrate on traditional biochemistry experiments and techniques. The manual also includes an introduction to ethics in the laboratory, uncommon in similar manuals. Most importantly, perhaps, is the authors' three-pronged approach to encouraging students to think like a research scientist: first, the authors introduce the scientific method and the hypothesis as a framework for developing conclusive experiments; second, the manual's experiments are designed to become increasingly complex in order to teach more advanced techniques and analysis; finally, gradually, the students are required to devise their own protocols. In this way, students and instructors are able to break away from a "cookbook" approach and to think and investigate for themselves. Suitable for lower-level and upper-level courses; Ninfa spans these courses and can also be used for some first-year graduate work.







Basic Laboratory Methods for Biotechnology


Book Description

Basic Laboratory Methods for Biotechnology, Third Edition is a versatile textbook that provides students with a solid foundation to pursue employment in the biotech industry and can later serve as a practical reference to ensure success at each stage in their career. The authors focus on basic principles and methods while skillfully including recent innovations and industry trends throughout. Fundamental laboratory skills are emphasized, and boxed content provides step by step laboratory method instructions for ease of reference at any point in the students’ progress. Worked through examples and practice problems and solutions assist student comprehension. Coverage includes safety practices and instructions on using common laboratory instruments. Key Features: Provides a valuable reference for laboratory professionals at all stages of their careers. Focuses on basic principles and methods to provide students with the knowledge needed to begin a career in the Biotechnology industry. Describes fundamental laboratory skills. Includes laboratory scenario-based questions that require students to write or discuss their answers to ensure they have mastered the chapter content. Updates reflect recent innovations and regulatory requirements to ensure students stay up to date. Tables, a detailed glossary, practice problems and solutions, case studies and anecdotes provide students with the tools needed to master the content.




Biochemistry Laboratory Manual For Undergraduates


Book Description

Biochemistry laboratory manual for undergraduates – an inquiry based approach by Gerczei and Pattison is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology while incorporating the blossoming field of bioinformatics. The novelty of this manual is the incorporation of a student-driven real real-life research project into the undergraduate curriculum. Since students test their own mutant design, even the most experienced students remain engaged with the process, while the less experienced ones get their first taste of biochemistry research. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.




Purification and Characterization of Secondary Metabolites


Book Description

Purification and Characterization of Secondary Metabolites: A Laboratory Manual for Analytical and Structural Biochemistry provides students with working knowledge of the fundamental and advanced techniques of experimental biochemistry. Sections provide an overview of the microbiological and biochemical methods typically used for the purification of metabolites and discuss the biological significance of secondary metabolites secreted by three diverse species of bacteria. Additionally, this lab manual covers the theory and practice of the most commonly-used techniques of analytical biochemistry, UV-vis and IR spectrophotometry, high-performance liquid chromatography, mass spectrometry, X-ray crystallography and nuclear magnetic resonance, and how to evaluate and effectively use scientific data. Instructors will find this book useful because of the modular nature of the lab exercises included. Written in a logical, easy-to-understand manner, this book is an indispensable resource for both students and instructors. - Offers project lab formats for students that closely simulate original research projects - Provides instructional guidance for students to design their own experiments - Presents advanced analytical techniques - Includes access to a website with additional resources for instructors