Bioluminescent Marine Plankton


Book Description

Bioluminescence, the “cold living light” or the “cold fire of the sea,” is extremely common in all oceans at all depths. However, this phenomenon is nearly absent in freshwater, with the exception of a freshwater limpet. More than 75% of deep-sea creatures have been reported to produce their own light. The luminescent marine plankton such as dinoflagellate, radiolarians, jellyfish, comb jellies, annelids, copepods, ostracods, mysids, amphipods, euphausiids, and tunicates form an important component in the marine food chain. Research on luminescent marine plankton is gaining momentum owing to its importance in life science research and medicine. The glowing Green Fluorescent Protein (GFP) extracted from the North Pacific jellyfish, Aqueorea victoria (for which the Japanese biologist, Osamu Shimonmura won the Nobel Prize in Chemistry in 2008) has helped shed light on key processes such as the spread of cancer, the development of brain cells, the growth of bacteria, damage to cells by Alzheimer's disease, and the development of insulin-producing cells in the pancreas. Therefore, it is difficult to underscore the importance of bioluminescence and the organisms associated with this phenomenon. Bioluminescent Marine Plankton is a unique reference that attempts to provide answers to questions about bioluminescence. The first of its kind, this book attempts to answer questions on the subject with a focus on planktonic organisms. 12 chapters provide information about the chemistry of bioluminescence, types of bioluminescent displays, distribution of bioluminescence among marine plankton, ecological functions and utility of planktonic bioluminescence. Chapters are dedicated to the biology and ecology of specific groups of plankton that span about 200 luminescent marine species. The detailed book is an essential compendium on marine plankton for a broad range of readers who want to learn about bioluminescent plankton. A list of detailed references is also provided for the benefit of teachers, students, researchers and enthusiasts interested in marine biology, aquaculture, and environmental sciences.




Bioluminescence: Chemical Principles And Methods (3rd Edition)


Book Description

This book is the bible of bioluminescence and a must-read not only for the students but for those who work in various fields relating to bioluminescence. It summarizes current structural information on all known bioluminescent systems in nature, from well-studied ones to those that have been seldom investigated.This book remains an important source of chemical knowledge on bioluminescence and, since the second edition's publication in 2012, has been revised to include major developments in two systems: earthworm Fridericia and higher fungi whose luciferins have been elucidated and synthesized. These two new luciferins represent an essential addition to seven previously known, with fully rewritten sections covering this new subject matter.




Photoproteins in Bioanalysis


Book Description

The use of light-emitting proteins for the detection of biomolecules provides fast and sensitive methods which overcome the disadvantages of radioactive labels and the high cost of fluorescent dyes. This reference work summarizes modern advanced techniques and their applications and includes practical examples of assays based on photoproteins. The book presents contemporary key topics like luminescent marine organisms, DNA probes, reporter gene assays and photoproteins, ratiometric sensing, use of photoproteins for in vivo functional imaging and luminescent proteins in binding assays, to name just a few, and is complemented by recent advances in instrumentation. Includes an introductory chapter by 2008 Chemistry Nobel laureate Osamu Shimomura.




Slow Church


Book Description

In today's fast-food world, Christianity can seem outdated or archaic. The temptation becomes to pick up the pace and play the game. But Chris Smith and John Pattison invites us to leave franchise faith behind and enter the kingdom of God, where people know each other well and love one another as Christ loves the church.




Ocean Drifters


Book Description

From the geology of the land around us to the weather and long-term climate, plankton affect our lives in ways of which few of us are aware. Discover this world beneath the waves.




The Eastern Mediterranean as a Laboratory Basin for the Assessment of Contrasting Ecosystems


Book Description

This book is the outcome of a NATO Advanced Research Workshop on "The Eastern Mediterranean as a laboratory basin for the assessment of contrasting ecosystems" that was held in Kiev, Ukraine, March 23-27, 1998. The scientific rationale of the workshop can be summarized as follows. The Eastern Mediterranean is the most nutrient impoverished and oligotrophic large water body known. There is a well-defined eastward trend in nutrient ratios over the entire Mediterranean that starts at the Gibraltar Straits and, through the western basin, proceeds to the Ionian and Levantine Seas. Supply of nutrients to the entire Mediterranean is limited by inputs from the North Atlantic and various river systems along the sea. The unique feature of the Mediterranean is the presence of an eastward longitudinal trend in available nitrate/phosphate ratios. This apparently induces a west-to-east variation in the structure of the pelagic food web and trophic interactions. In this context the Mediterranean, and in particular its Eastern basin, provides probably a unique platform to explore the hypotheses related to the suggested phosphate-limitation on production and to the shift between "microbial" and "classical" modes of operation of the photic food web. The major exception of the overall oligotrophic nature of the Eastern Mediterranean is the highly eutrophic system of the Northern Adriatic Sea. Here, during the last two decades the discharges of the northern rivers (especially of the Po), together with municipal sewage, have led to a very marked increase of nutrients and subsequent imponent eutrophication events.




Algal Culturing Techniques


Book Description

Algal Culturing Techniques is a comprehensive reference on all aspects of the isolation and cultivation of marine and freshwater algae, including seaweeds. It is divided into seven parts that cover history, media preparation, isolation and purification techniques, mass culturing techniques, cell counting and growth measurement techniques, and reviews on topics and applications of algal culture techniques for environmental investigations. Algal Culturing Techniques was developed to serve as both a new textbook and key reference for phycologists and others studying aquatic systems, aquaculture and environmental sciences. Students of algal ecology, marine botany, marine phycology, and microbial ecology will enjoy the hands-on methodology for culturing a variety of algae from fresh and marine waters. Researchers in industry, such as aquaculture, pharmaceutical, foodstuffs, and biotechnology companies will find an authoritative and comprehensive reference. - Sponsored by the Phycological Society of America - Features color photographs and illustrations throughout - Describes culturing methods ranging from the test tube to outdoor ponds and coastal seaweed farms - Details isolation techniques ranging from traditional micropipette to automated flow cytometeric methods - Includes purification, growth, maintenance, and cryopreservation techniques - Highlights methods for estimating algal populations, growth rates, isolating and measuring algal pigments, and detecting and culturing algal viruses - Features a comprehensive appendix of nearly 50 algal culture medium recipes - Includes a glossary of phycological terms







Bioluminescence


Book Description

This volume presents detailed laboratory protocols regarding the three major route technologies luciferases, efficient optical probes, and applications to visualizing molecular events in living subjects. Bioluminescence: Methods and Protocols, Third Edition guides readers through chapters on ingredients of bioluminescent probes, and fabrication of bioluminescent probes, applications to living subjects and instrumentations. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Bioluminescence: Methods and Protocols, Third Edition is a useful complement to the first and second edition for new and experienced researchers alike.




Plankton


Book Description

Healthy waterways and oceans are essential for our increasingly urbanised world. Yet monitoring water quality in aquatic environments is a challenge, as it varies from hour to hour due to stormwater and currents. Being at the base of the aquatic food web and present in huge numbers, plankton are strongly influenced by changes in environment and provide an indication of water quality integrated over days and weeks. Plankton are the aquatic version of a canary in a coal mine. They are also vital for our existence, providing not only food for fish, seabirds, seals and sharks, but producing oxygen, cycling nutrients, processing pollutants, and removing carbon dioxide from our atmosphere. This Second Edition of Plankton is a fully updated introduction to the biology, ecology and identification of plankton and their use in monitoring water quality. It includes expanded, illustrated descriptions of all major groups of freshwater, coastal and marine phytoplankton and zooplankton and a new chapter on teaching science using plankton. Best practice methods for plankton sampling and monitoring programs are presented using case studies, along with explanations of how to analyse and interpret sampling data. Plankton is an invaluable reference for teachers and students, environmental managers, ecologists, estuary and catchment management committees, and coastal engineers.