Development of the Rat Spinal Cord: Immuno- and Enzyme Histochemical Approaches


Book Description

The studies described here were carried out in the Neuroregul ation Group, Department of Physiology, University of Leiden, the Netherlands. Over the last decade, this group, in close collaboration with the Department of Neurosurgery of the Academic Hospital of Leiden, has studied the development of the central nervous system from a neuroanatomical as well as a clinical perspective. During this period, the expression of several morphore gulators in the developing rat spinal cord was extensively investigated. Parallel studies focused on the development of the spinal cord fiber systems, which was studied by means of the intrauterine use of neuronal tracers. The main goal of these studies was to extend our knowledge about the (normal) generation of the spinal cord and to contribute to the under standing of clinical problems related to regeneration and degeneration in the mammalian central nervous system. The studies on morphoregulators, in particular, appeared to benefit two different scientific areas. Firstly, the correlation between morphoregulator expression patterns and known anatomy contributed to our knowledge about spinal cord development. Secondly, the correlation between morpho regulator expression patterns and known developmental processes may help to understand their precise function(s). This volume of Advances in Anatomy, Embryology and Cell Biology presents these particular studies on the development of the rat spinal cord performed over the last decade. As well as integrating the results of the tracer studies, this volume also provides an update on the development of the rat spinal cord.




Development of the Rat Spinal Cord


Book Description

This edition provides an update on the latest findings on the development ofthe rat spinal cord. The expression pat- terns of several morphoregulators were investigated using immuno-/histochemistry and are discussed in relationto the known anatomy of the spinal cord. The embryonal development of different fiber systems was studied using a newly develo- ped intrauterine tracing technique. In a multidisciplinary effort the different studies were integrated and new ideas on the developmental events in the rat spinal cord discus- sed.




Sensory Mechanisms of the Spinal Cord


Book Description

The third edition of this monograph continues to have the goal of providing an overview of current thought about the spinal cord mechanisms that are responsible for sensory processing. We hope that the book is of value to both basic and clinical neuroscientists. Several changes have been made in the presentation, as well as additions because of the research advances that have been made during the past decade. Chapters 3 and 4 in the previous edition have been subdivided, and now the morphology of primary afferent neu rons of the dorsal root ganglia is described in Chapter 3 and the chemical neuroanatomy 4. The description of the dorsal hom in the previous Chapter 4 of these neurons in Chapter is now included in Chapter 5, and the chemical neuroanatomy of the dorsal hom in Chapter 6. Furthermore, discussions of the descending control systems have now been of Chapter 12. consolidated at the end The authors would like to express their appreciation for the help provided by several individuals. R.E.C. wishes to acknowledge the many things he learned about primary afferent neurons from conversations with Dr S. N. Lawson. He also thanks Lyn Shilling for her assistance with the typing. WDW thanks Dr Nada Lawand for her critical reading of parts of the manuscript, Rosaline Leigh for help with the manuscript, and Griselda Gonzales for preparing the illustrations.







The Projections to the Spinal Cord of the Rat During Development: A Timetable of Descent


Book Description

The studies described here were carried out in the Neuroregulation Group at the Department of Physiology of the University of Leiden. Over the last decade this group has, in close collaboration with the departments of neurosurgery and urology of the Academic Hospital of Leiden, studied development and regeneration of the spinal cord and its peripheral nerves both from a neuroanatomical and a clinical perspective. During this period the development of brainstem projections to the spinal cord of the rat was studied with a retrograde tracing technique. Horseradish peroxidase was injected into the spinal cord of rat fetuses, both at different ages, and at different levels of the spinal cord. These studies aimed to discover regularities in the behavior of descending fiber systems that could yield insight into the logic that the nervous system must employ to structure its connectional pattern during development. Such insight might then be applied to improve regeneration of the nervous system.




Enzyme Histochemistry


Book Description

During recent years enzyme histochemical reactions have increasingly been considered as important, the reason being that enzyme histo chemistry is now a well-established link between morphology and bio chemistry. The development of numerous new methods and in particular the improvement of existing techniques contributed to the expansion of enzyme histochemical reactions. Today, the use of these methods allows detailed insight into molecular processes of single cells and their constituents. The selection of a suitable method for enzyme histochemical investigations needs thorough knowledge and critical evaluation of the reactions de scribed for the histochemical demonstration of enzymes and introduced in laboratory practice. Often, it is difficult for scientists primarily concerned with the application of methods and for laboratory assistants to comment on the value of an enzyme histochemical reaction. Our book will serve as a guide in this respect. It contains the most important histochemical methods for the localization of enzymes, all of which were checked by the authors themselves. These methods were often modified and frequently used for numerous different investigations of healthy and diseased organs in basic research and in routine practice.




Research Awards Index


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Research Grants Index


Book Description




Head and Neck


Book Description

This book offers a critical review of the head and neck from an anatomical, physiological and clinical perspective. It begins by providing essential anatomical and physiological information, then discusses historical and current views on specific aspects in subsequent chapters. For example, the anatomy of the skull cap or cranial vault provided in the first chapter is discussed in the context of malformation and identity, as well as the development of the bony skull, in the following chapters. These chapters provide stepping-stones to guide readers through the book. There are new fields of research and technological developments in which Anatomy and Physiology lose track of progress. One of the examples discussed is the automated face recognition. In some respects, e.g. when it comes to cancers and malformations, our understanding of the head and neck – and the resulting therapeutic outcomes – have been extremely disappointing. In others, such as injuries following car accidents, there have been significant advances in our understanding of head and neck dysfunctions and their treatment. Therefore head movements, also during sleep, and head and neck reflexes are discussed. The book makes unequivocal distinctions between correct and incorrect assumptions and provides a critical review of alternative clinical methods for head and neck dysfunctions, such as physiotherapy and lymphatic drainage for cancers. Moreover, it discusses the consequences of various therapeutic measures for physiological and biomechanical conditions, as well as puberty and aging. Lastly, it addresses important biomedical engineering developments for hearing e.g. cochlear implants and for applying vestibular cerebellar effects for vision.