Controlling and Modeling Phalaenopsis Orchid Spike Elongation as a Function of Temperature and Plant Growth Regulator Application


Book Description

Phalaenopsis orchids are one of the most important potted flowering plants in floriculture. In the first part of our study, we developed a model to simulate the spike elongation of Phalaenopsis `KV Beauty' in response of various temperatures ranging from 15C to 25C. A modified Richards' function was used to predict the daily increment of spike elongation, and the change of parameters' values in relation to the change of temperatures were described with two quadratic functions. The simulation model fitted the observed data fairly well with an R-square = 0.994 in the validation test. The total days required to flower decreased as temperature increased; however, the number of flowers significantly decreased under 21C and 25C. In the second part of our study, we evaluated the effects of two plant growth retardants (paclobutrazol and flurprimidol) on spike elongation of Phalaenopsis `KV Beauty' with different application methods and concentrations. Spraying on the leaves and applying directly to the spikes of 200 mg/L paclobutrazol shortened the total spike lengths by 15% when applied on plants with spikes 7-10 cm long. In another experiment, applying 100 mg/L and 200 mg/L paclobutrazol and flurprimidol on spikes both significantly shortened total spike lengths up to 77% when applied on plants with spikes 1-2 cm. Applying paclobutrazol resulted in shorter internodes between flowers, whereas applying flurprimidol resulted in shorter internodes between the base of the spike to the first flower. Such result indicated that paclobutrazol and flurprimidol had different function mechanisms in shortening plant internodes. Applying flurprimidol directly to the spike could potentially be applied to the industry to control the final spike lengths and to reduce the production costs.




The Orchid Genome


Book Description

This book provides information on genome complexity and evolution, transcriptome analysis, miRNome, simple sequence repeats, genome relationships, molecular cytogenetics, polyploidy induction and application, flower and embryo development. Orchids account for a great part of the worldwide floriculture trade both as cut flowers and as potted plants and are assessed to comprise around 10% of global fresh cut flower trade. A better understanding of the basic botanical characteristics, flower regulation, molecular cytogenetics, karyotypes and DNA content of important orchids will aid in the efficient development of new cultivars. The book also describes the composition, expression and function of various microRNAs and simple sequence repeats. Information on their involvement in all aspects of plant growth and development will aid functional genomics studies.




Effects of Plant Growth Regulators and Temperature on Floral Induction and Development of Exacum Styer Group


Book Description

Plants of Exacum Styer Group, interspecific hybrids from five Sri Lankan taxa from the Gentian family, have significant horticultural potential as flowering potted plants, bedding plants and cut flowers. However, a better understanding of the requirements for floral induction and flower development is needed before commercialization. Experiments examining the impact of plant growth regulators (PGRs) on floral induction and subsequent plant development consisted of seven treatments: ethephon (500 and 1000 PPM), daminozide (2500 and 3500 PPM), gibberellin (GA4+-- at 10 and 15 PPM), and a control of water plus surfactant. Analysis of data collected on weeks to anthesis resulted in a significant interaction between treatment and genotype indicating that genotypes did not behave uniformly across treatments. Overall, the effect of PGR treatments on flowering of E. Styer Group is impacted more by genotype than by chemical with late flowering genotypes less affected by chemical than earlier flowering genotypes. Nonetheless, daminozide effectively reduced stem length without dramatic negative impact on flowering and can be considered safe for commercial production. Experiments to evaluate the effect of temperature on floral induction and subsequent plant development consisted of four temperature treatments over two seasons (one treatment repeated in both seasons). The two most extreme treatments prevented flowering for some or all genotypes (35° C DT/31° C NT and 16° C DT/12° C NT, respectively). Intermediate treatments (23° C DT/19° C NT and 30° C/26° C NT) were optimal for fastest flowering depending on genotype. As with the response to PGR treatments, significant genotypic variation was observed in response to temperature indicating production schemes must be developed empirically for each individual genotype. However, the optimal temperature for fast flowering and attractive plant conformation is likely between 21° and 28° C average daily temperature for most E. Styer Gro.







Effects of Nutrient Supply and Cooling on Growth, Flower Bud Differentiation, and Propagation of the Nobile Dendrobium Orchid


Book Description

Studies of Dendrobium Sea Mary 0́Snow King0́9 investigated the effect of nutrient termination (1 Aug., 1 Sept., or 1 Oct.) and reapplication [at the beginning, in the middle, immediately after, or 2 weeks after (relative to cooling), or no nutrient reapplication] on growth and flowering, quantified cooling requirements (10, 13, 15, or 18 °C for 2 to 6 weeks) for flowering, and determined optimum nutrient termination (on the three above dates) and nutrient rate (0.33, 0.67, or 1.33 g0́ØL-1 15N-2.3P-12.9K) for producing single-node cuttings. Regardless of reapplication stages, nutrient termination on 1 Oct. caused taller plants with more nodes, more leaves, more flowering nodes, more total flowers, and fewer aborted flowers than those being terminated earlier. Only buds protruding above 2 mm from pseudobulb surface showed differentiated floral structures. Plants with 1 Aug. nutrient termination had larger flower primordia than those with 1 Oct., indicating flower differentiated earlier or faster with an earlier nutrient termination. No reversion of reproductive to vegetative buds arose due to either late nutrient termination or resumption of nutrients during cooling. Interactions between temperature and cooling duration were significant on time required for anthesis and full flowering, recorded from either beginning or completion of cooling, average flower number per flowering node, and flower diameter. Increasing cooling duration from 2 to 6 weeks led plants to reach anthesis and full flowering faster after cooling; however, the increasing cooling duration actually extended total time for producing flowering crops. Increasing temperature from 10 to 15 °C accelerated flowering after cooling. Plants had more flowering nodes and total flowers when cooled at 10 to 15 °C than at 18 °C. The results suggest that 3 weeks of cooling at 13 or 15 °C produce quality flowering plants that require less time to reach flowering. Plants fertilized at 0.67 or 1.33 g0́ØL-1 were taller with 18% more nodes and more leaves than those receiving 0.33 g0́ØL-1. Increasing nutrient rate with prolonged supply to the plants caused more single-node cuttings to grow into vegetative shoots for propagation, fewer cuttings to transition to flowering nodes, and less flower abortion to occur.




Orchid Biology


Book Description

A Personal Note I decided to initiate Orchid Biology: Reviews and Perspectives in about 1972 and (alone or with co-authors) started to write some of the chapters and the appendix for the volume in 1974 during a visit to the Bogor Botanical Gardens in Indonesia. Professor H. C. D. de Wit of Holland was also in Bogor at that time and when we discovered a joint interest in Rumphius he agreed to write a chapter about him. I visited Bangkok on my way home from Bogor and while there spent time with Professor Thavorn Vajrabhaya. He readily agreed to write a chapter. The rest of the chapters were solicited by mail and I had the complete manuscript on my desk in 1975. With that in hand I started to look for a publisher. Most of the publishers I contacted were not interested. Fortunately Mr James Twiggs, at that time editor of Cornell University Press, grew orchids and liked the idea. He decided to publish Orchid Biology: Reviews and Per spectives, and volume I saw the light of day in 1977. I did not know if there would be a volume II but collected manuscripts for it anyway. Fortunately volume I did well enough to justify a second book, and the series was born. It is still alive at present - 20 years, seven volumes and three publishers later. I was in the first third of my career when volume I was published.