Escherichia coli O157:H7 in Ground Beef


Book Description

USDA's Food Safety and Inspection Service (FSIS) is formulating risk assessments to identify important foodborne hazards; evaluate potential strategies to prevent, reduce, or eliminate those hazards; assess the effects of different mitigation strategies; and identify research needs. These risk assessments, in brief, empirically characterize the determinants of the presence or level of microbial contamination in vulnerable foodstuffs at various points leading up to consumption. One of the initial efforts in the undertaking is a risk assessment of the public health impact of E. coli O157:H7 in ground beef. In addition to soliciting public input, FSIS asked the Institute of Medicine (IOM) to convene a committee of experts to review the draft and offer recommendations and suggestions for consideration as the agency finalizes the document. This report presents the results of that review.




Escherichia Coli 0157:H7 in Ground Beef


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An Update


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Effects of Soluble Polylactic Acid and Gamma Irradiation on Ground Beef Inoculated with Escherichia Coli O157:H7 and Legal Classification of Irradiation as a Food Additive


Book Description

Ground beef samples were inoculated with stationary phase E. coli 0157:H7 cells to a concentration of 10 7 cfu/ml and allowed to incubate for 24 hours. The samples were treated with a 2% solution of soluble polylactic acid (SPLA) and exposed to gamma radiation in doses ranging from 7-125 krads at a dose rate of 17.1 krads/hr. After 2 days of storage at 4°C there was an overall reduction in E. coli 0157:H7 populations of 0.70 log 10 CFU/ml. (30%) in ground beef treated with SPLA only. Gamma irradiation was effective in reducing E. coli 0157:H7 populations in ground beef and resulted in an overall reduction of 4.55 log 10 CFU/ml after 125 krads. SPLA in combination with gamma radiation was less effective than gamma radiation alone in reducing populations of E. coli 0157:H7 in ground beef and resulted in a reduction in E. coli 0157:H7 populations of 3.80 log 10 CFU/ml after 125 krads. SPLA was protective at the 2% level and may act to prevent the cleavage of DNA molecules. Refrigerated storage of irradiated ground beef at 4°C for 7 days had no significant effect on E. coli 0157:H7 populations. SPLA treatment had a significant effect (p