Evaluation and validation of rapid molecular methods for the detection and identification of microorganisms in water - Standard Operating Procedures


Book Description

Molecular techniques are emerging as a rapid and powerful approach for microorganism detection. This project reviewed the efficacy of PCR assays for Cryptosporidium, Microcystis, adenovirus and ammonia oxidising bacteria, as well as candidate techniques for DNA extraction and inhibitor removal. The literature review led to an evaluation of DNA extraction kits and reagents for PCR; finalisation of assay formats; the development of PCR controls and Standard Operating Procedures (SOP); analysis of assay robustness using real samples; and completion of an Inter-laboratory trial using the SOP’s developed. This book is co-published with Water Research Australia.




Indicators for Waterborne Pathogens


Book Description

Recent and forecasted advances in microbiology, molecular biology, and analytical chemistry have made it timely to reassess the current paradigm of relying predominantly or exclusively on traditional bacterial indicators for all types of waterborne pathogens. Nonetheless, indicator approaches will still be required for the foreseeable future because it is not practical or feasible to monitor for the complete spectrum of microorganisms that may occur in water, and many known pathogens are difficult to detect directly and reliably in water samples. This comprehensive report recommends the development and use of a "tool box" approach by the U.S Environmental Protection Agency and others for assessing microbial water quality in which available indicator organisms (and/or pathogens in some cases) and detection method(s) are matched to the requirements of a particular application. The report further recommends the use of a phased, three-level monitoring framework to support the selection of indicators and indicator approaches.Â




Microbial Threats to Health


Book Description

Infectious diseases are a global hazard that puts every nation and every person at risk. The recent SARS outbreak is a prime example. Knowing neither geographic nor political borders, often arriving silently and lethally, microbial pathogens constitute a grave threat to the health of humans. Indeed, a majority of countries recently identified the spread of infectious disease as the greatest global problem they confront. Throughout history, humans have struggled to control both the causes and consequences of infectious diseases and we will continue to do so into the foreseeable future. Following up on a high-profile 1992 report from the Institute of Medicine, Microbial Threats to Health examines the current state of knowledge and policy pertaining to emerging and re-emerging infectious diseases from around the globe. It examines the spectrum of microbial threats, factors in disease emergence, and the ultimate capacity of the United States to meet the challenges posed by microbial threats to human health. From the impact of war or technology on disease emergence to the development of enhanced disease surveillance and vaccine strategies, Microbial Threats to Health contains valuable information for researchers, students, health care providers, policymakers, public health officials. and the interested public.




Bacteriological Analytical Manual


Book Description




Heterotrophic Plate Counts and Drinking-water Safety


Book Description

Heterotrophic Plate Counts and Drinking-water Safety provides a critical assessment of the role of the Heterotrophic Plate Count (HPC) measurement in drinking water quality management. It was developed from an Expert workshop of 32 scientists convened by the World Health Organization and the WHO/NSF International Collaborating Centre for Drinking Water Safety and Treatment in Geneva, Switzerland. Heterotrophs are organisms, including bacteria, yeasts and moulds, that require an external source of organic carbon for growth. The HPC test (or Standard Plate Count), applied in many variants, is the internationally accepted test for measuring the hetrotrophic microorganism population in drinking water, and also other media. It measures only a fraction of the microorganisms actually present and does not distinguish between pathogens and non-pathogens. High levels of microbial growth can affect the taste and odor of drinking water and may indicate the presence of nutrients and biofilms which could harbor pathogens, as well as the possibility that some event has interfered with the normal production of the drinking water. HPC counts also routinely increase in water that has been treated by an in-line device such as a carbon filter or softener, in water-dispensing devices and in bottled waters and indeed in all water that has suitable nutrients, does not have a residual disinfectant, and is kept under sufficient conditions. There is debate among health professionals as to the need, utility or quantitative basis for health-based standards or guidelines relating to HPC-measured regrowth in drinking water. The issues that were addressed in this work include: the relationship between HPC in drinking water (including that derived from in-line treatment systems, dispensers and bottled water) and health risks for the general public the role of HPC as an indirect indicator or index for pathogens of concern in drinking water the role of HPC in assessing the efficacy and proper functioning of water treatment and supply processes the relationship between HPC and the aesthetic acceptability of drinking water. Heterotrophic Plate Counts and Drinking-water Safety provides valuable information on the utility and the limitations of HPC data in the management and operation of piped water systems as well as other means of providing drinking water to the public. It is of particular value to piped public water suppliers and bottled water suppliers, manufacturers and users of water treatment and transmission equipment and inline treatment devices, water engineers, sanitary and clinical microbiologists, and national and local public health officials and regulators of drinking water quality.




Microbial Source Tracking


Book Description

Presents a state-of-the-art review of the current technology and applications being utilized to identify sources of fecal contamination in waterways. - Serves as a useful reference for researchers in the food industry, especially scientists investigating etiological agents responsible for food contamination. - Provides background information on MST methods and the assumptions and limitations associated with their use. - Covers a broad range of topics related to MST, including environmental monitoring, public health and national security, population biology, and microbial ecology. - Offers valuable insights into future research directions and technology developments.




Microbiology Laboratory Guidebook


Book Description




Industrial and Environmental Biotechnology


Book Description

The contamination of the environment by herbicides, pesticides, solvents, various industrial byproducts (including toxic metals, radionucleotides and metalloids) is of enormous economic and environmental significance. Biotechnology can be used to develop "green" or environmentally friendly solutions to these problems by harnessing the ability of bacteria to adapt metabolic pathways, or recruit new genes to metabolise harmful compounds into harmless byproducts. In addition to its role in cleaning-up the environment, biotechnology can be used for the production of novel compounds with both agricultural and industrial applications. Internationally acclaimed authors from diverse fields present comprehensive reviews of all aspects of Industrial and Environmental Biotechnology. Chapters concerned with environmental biotechnology cover two major categories of pollutants: organic compounds and metals. Organic pollutants include cyclic aromatic compounds, with/without nitrogenous or chloride substitutions while metal pollutants include copper, chromate, silver, arsenic and mercury. The genetic basis of bioremediation and the microbial processes involved are examined, and the current and/or potential applications of bioremediation are discussed. The use of biotechnology for industrial and agricultural applications includes a chapter on the use of enzymes as biocatalysts to synthesize novel opiate derivatives of medical value. The conversion of low-value molasses to higher value products by biotechnological methods and the use tissue culture methods to improve sugar cane and potatoes crop production is discussed.




Microbial Forensics


Book Description

Microbial Forensics is a rapidly evolving scientific discipline. In the last decade, and particularly due to the anthrax letter attacks in the United States, microbial forensics has become more formalized and has played an increasingly greater role in crime investigations. This has brought renewed interest, development and application of new technologies, and new rules of forensic and policy engagement. It has many applications ranging from biodefense, criminal investigations, providing intelligence information, making society more secure, and helping protect precious resources, particularly human life. A combination of diverse areas is investigated, including the major disciplines of biology, microbiology, medicine, chemistry, physics, statistics, population genetics, and computer science. Microbial Forensics, Second Edition is fully revised and updated and serves as a complete reference of the discipline. It describes the advances, as well as the challenges and opportunities ahead, and will be integral in applying science to help solve future biocrimes. - A collection of microbiology, virology, toxicology and mycology as it relates to forensics, in one reference - New and expanded content to include statistical analysis of forensic data and legal admissibility and the standards of evidence, to name a few - Includes research information and application of that research to crime scene analysis, which will allow practitioners to understand and apply the knowledge to their practice with ease




Management of Legionella in Water Systems


Book Description

Legionnaires' disease, a pneumonia caused by the Legionella bacterium, is the leading cause of reported waterborne disease outbreaks in the United States. Legionella occur naturally in water from many different environmental sources, but grow rapidly in the warm, stagnant conditions that can be found in engineered water systems such as cooling towers, building plumbing, and hot tubs. Humans are primarily exposed to Legionella through inhalation of contaminated aerosols into the respiratory system. Legionnaires' disease can be fatal, with between 3 and 33 percent of Legionella infections leading to death, and studies show the incidence of Legionnaires' disease in the United States increased five-fold from 2000 to 2017. Management of Legionella in Water Systems reviews the state of science on Legionella contamination of water systems, specifically the ecology and diagnosis. This report explores the process of transmission via water systems, quantification, prevention and control, and policy and training issues that affect the incidence of Legionnaires' disease. It also analyzes existing knowledge gaps and recommends research priorities moving forward.