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Experimental Protocols in Biotechnology

Author : Neelima Gupta

Publisher : Humana

Page : 246 pages

File Size : 33,11 MB

Release : 2021-06-30

Category : Science

ISBN : 9781071606094

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Book Description

This manual provides insights into the theory and practical aspects of several biotechnological and biochemical techniques for plants, protozoa, nematodes, insects and fishes, as well as human samples. The book also covers bioinformatics tools. The manual is an inclusive compilation, explaining techniques for microbial cultures, their diagnostics, DNA barcoding, microscopic techniques, blood analysis, parasite diagnostics through copro-antigens, enzyme analysis with enzyme kinetics, gene expression analysis, in-vivo protein visualization in live animals, geno-toxicity assays, quantification of micro RNAs and LncRNAs in tissue sections, the use of droplet PCR, and in-silico analysis. It provides step-by-step descriptions and details of each methodology, together with the final outcomes and inferences, in a simple and lucid manner, easily reproducible even for beginners. The broad range of techniques covered makes this volume extremely useful in understanding the principles of biotechniques, and simple applications for practical courses.



Molecular Biology and Biotechnology

Author : M. P. Bansal

Publisher : The Energy and Resources Institute (TERI)

Page : 392 pages

File Size : 14,4 MB

Release : 2013-01-01

Category : Science

ISBN : 8179933792

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Book Description

Molecular Biology and Biotechnology: basic experimental protocols is a compilation of methods and techniques commonly used in biomedical and biotechnological studies. The book aims to provide ample support to both students and faculty while conducting practical lessons. Four sections are covered in this book—Genomics, Proteomics, Quantitative Biochemistry, and Bioinformatics. A concise introductory note accompanies each protocol/method described for better comprehension. Every topic discussed is supported by actual methods and their expected results, and is accompanied by relevant questions.





Methods in Biotechnology

Author : Seung-Beom Hong

Publisher : John Wiley & Sons

Page : 360 pages

File Size : 37,17 MB

Release : 2016-05-12

Category : Science

ISBN : 1119156793

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Book Description

As rapid advances in biotechnology occur, there is a need for a pedagogical tool to aid current students and laboratory professionals in biotechnological methods; Methods in Biotechnology is an invaluable resource for those students and professionals. Methods in Biotechnology engages the reader by implementing an active learning approach, provided advanced study questions, as well as pre- and post-lab questions for each lab protocol. These self-directed study sections encourage the reader to not just perform experiments but to engage with the material on a higher level, utilizing critical thinking and troubleshooting skills. This text is broken into three sections based on level – Methods in Biotechnology, Advanced Methods in Biotechnology I, and Advanced Methods in Biotechnology II. Each section contains 14-22 lab exercises, with instructor notes in appendices as well as an answer guide as a part of the book companion site. This text will be an excellent resource for both students and laboratory professionals in the biotechnology field.



Experimental Design for Biologists

Author : David J. Glass

Publisher : CSHL Press

Page : 211 pages

File Size : 25,94 MB

Release : 2007

Category : Biology

ISBN : 0879697350

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Book Description

The effective design of scientific experiments is critical to success, yet graduate students receive very little formal training in how to do it. Based on a well-received course taught by the author, Experimental Design for Biologistsfills this gap. Experimental Design for Biologistsexplains how to establish the framework for an experimental project, how to set up a system, design experiments within that system, and how to determine and use the correct set of controls. Separate chapters are devoted to negative controls, positive controls, and other categories of controls that are perhaps less recognized, such as “assumption controls†and “experimentalist controls†. Furthermore, there are sections on establishing the experimental system, which include performing critical “system controls†. Should all experimental plans be hypothesis-driven? Is a question/answer approach more appropriate? What was the hypothesis behind the Human Genome Project? What color is the sky? How does one get to Carnegie Hall? The answers to these kinds of questions can be found in Experimental Design for Biologists. Written in an engaging manner, the book provides compelling lessons in framing an experimental question, establishing a validated system to answer the question, and deriving verifiable models from experimental data. Experimental Design for Biologistsis an essential source of theory and practical guidance in designing a research plan.



Cell Biology Protocols

Author : J. Robin Harris

Publisher : Wiley

Page : 418 pages

File Size : 26,50 MB

Release : 2012-01-18

Category : Science

ISBN : 9780470033463

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Book Description

As a modern composite scientific discipline, Cell Biology has expanded and moved forward rapidly in recent years. Cell Biologists now require a wide range of techniques, including those of analytical biochemistry and microscopy in all its diverse forms. These are often used alongside the techniques of molecular biology and molecular genetics. This book contains numerous useful protocols, covering light and electron microscopy, cell culture, cell separation, subcellular fractionation, organelle and membrane isolation, and the use of in vitro reassembly systems in Cell Biology. Many of these protocols feature helpful notes and safety information for practical application. The format favours easy use at the bench with space for notes and important safety information. An appendix contains essential analytical information that will prove invaluable to those working on all aspects of cell biology. This book will be of interest to students and more experienced cell biologists, as well as molecular biologists and those working in genomics and proteomics who are looking for cellular techniques to validate their findings within intact cells.



Advanced Methods in Molecular Biology and Biotechnology

Author : Khalid Z. Masoodi

Publisher : Academic Press

Page : 220 pages

File Size : 11,16 MB

Release : 2020-10-28

Category : Science

ISBN : 012824450X

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Book Description

Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. - Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology - Features clear, step-by-step instruction for applying the techniques covered - Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment



Preparing for Future Products of Biotechnology

Author : National Academies of Sciences, Engineering, and Medicine

Publisher : National Academies Press

Page : 231 pages

File Size : 24,66 MB

Release : 2017-07-28

Category : Science

ISBN : 0309452058

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Book Description

Between 1973 and 2016, the ways to manipulate DNA to endow new characteristics in an organism (that is, biotechnology) have advanced, enabling the development of products that were not previously possible. What will the likely future products of biotechnology be over the next 5â€"10 years? What scientific capabilities, tools, and/or expertise may be needed by the regulatory agencies to ensure they make efficient and sound evaluations of the likely future products of biotechnology? Preparing for Future Products of Biotechnology analyzes the future landscape of biotechnology products and seeks to inform forthcoming policy making. This report identifies potential new risks and frameworks for risk assessment and areas in which the risks or lack of risks relating to the products of biotechnology are well understood.



Laboratory Protocols in Applied Life Sciences

Author : Prakash Singh Bisen

Publisher : CRC Press

Page : 1772 pages

File Size : 25,61 MB

Release : 2014-02-26

Category : Medical

ISBN : 1466553154

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Book Description

As applied life science progresses, becoming fully integrated into the biological, chemical, and engineering sciences, there is a growing need for expanding life sciences research techniques. Anticipating the demands of various life science disciplines, Laboratory Protocols in Applied Life Sciences explores this development. This book covers a wide spectrum of areas in the interdisciplinary fields of life sciences, pharmacy, medical and paramedical sciences, and biotechnology. It examines the principles, concepts, and every aspect of applicable techniques in these areas. Covering elementary concepts to advanced research techniques, the text analyzes data through experimentation and explains the theory behind each exercise. It presents each experiment with an introduction to the topic, concise objectives, and a list of necessary materials and reagents, and introduces step-by-step, readily feasible laboratory protocols. Focusing on the chemical characteristics of enzymes, metabolic processes, product and raw materials, and on the basic mechanisms and analytical techniques involved in life science technological transformations, this text provides information on the biological characteristics of living cells of different origin and the development of new life forms by genetic engineering techniques. It also examines product development using biological systems, including pharmaceutical, food, and beverage industries. Laboratory Protocols in Applied Life Sciences presents a nonmathematical account of the underlying principles of a variety of experimental techniques in disciplines, including: Biotechnology Analytical biochemistry Clinical biochemistry Biophysics Molecular biology Genetic engineering Bioprocess technology Industrial processes Animal Plant Microbial biology Computational biology Biosensors Each chapter is self-contained and written in a style that helps students progress from basic to advanced techniques, and eventually design and execute their own experiments in a given field of biology.



Carbohydrate Biotechnology Protocols

Author : Christopher Bucke

Publisher : Springer Science & Business Media

Page : 334 pages

File Size : 50,94 MB

Release : 2008-02-05

Category : Science

ISBN : 1592592619

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Book Description

We are in a phase of the evolution of biotechnology in which the true and potential commercial importance of carbohydrates is becoming appre- ated more fully. Progress in providing hard facts to establish the commercial value ofpolysaccharides and oligosaccharides is limited, as always, by lack of funding and by a relative shortage of skilled practitioners in the production and analysis of those materials. Carbohydrate science has a reputation, not unmerited, for technical difficulty owing to the structural similarity of the many monosaccharide monomers and the potential, and real, complexity of oligosaccharides and polysaccharides, particularly heterosaccharides conta- ing many different monomers. Modem analytical and synthetic methods, in many cases using enzyme technology, are beginning to allow this complexity to be unraveled. Carbohydrate Biotechnology Protocols is aimed at those newcomers who have an interest in the production and use of carbohydrate materials, but have shied away from involvement for lack of detailed descriptions of appropriate methods, including the type of practical hints that may be provided by those skilled in those methods, but that are rarely described in research papers. The majority of the contributions to this book conform to the established format of the Methods in Biotechnology series. They begin with the theoretical and c- mercial background to the method or group of methods, provide a list of the reagents and equipment required for the procedure, then give a detailed st- by-step description of how to carry out the protocol.