Gas Chromatography and Lipids


Book Description

Fatty acids and lipids: structures, extraction and fractionation into classes -- Gas chromatography: theoretical aspects and instrumentation -- Preparation of methyl ester and other derivatives -- Gas chromatographic analysis of fatty acid derivatives -- Isolation of fatty acids and identification by spectroscopic and chemical degradative techniques -- Gas chromatography--mass spectrometry and fatty acids -- Gas chromatographic analysis of molecular species of lipids -- Alternative or complementary methods for the analysis of molecular species of lipids -- Some miscellaneous separations of lipids by gas chromatography.




Lipid Analysis


Book Description

This well-known and highly successful book was first published in 1973 and has been completely re-written in subsequent editions (published in 1982 and 2003). This new Fourth Edition has become necessary because of the pace of developments in mass spectrometry of intact lipids, which has given recognition of lipid analysis and ‘lipidomics’ as a distinct science. To bring the book up to date with these developments, author William W. Christie is joined by co-author Xianlin Han. Although devoting considerable space to mass spectrometry and lipidomics, Lipid analysis remains a practical guide, in one volume, to the complexities of the analysis of lipids. As in past editions, it is designed to act as a primary source, of value at the laboratory bench rather than residing on a library shelf. Lipid analysis deals with the isolation, separation, identification and structural analysis of glycerolipids, including triacylglycerols, phospholipids, sphingolipids, and the various hydrolysis products of these. The chapters follow a logical sequence from the extraction of lipids to the isolation and characterization of particular lipid classes and of molecular species of each, and to the mass spectrometric analysis of lipids and lipidomics. The new influence of mass spectrometry is due mainly to the development of electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). Most emphasis in this book is placed on ESI, which is enabling structural characterization of different lipid classes and the identification of novel lipids and their molecular species.




Plant Lipids


Book Description

This volume explores analytical methods to study complex lipid mixtures from plants and algae. The chapters in this book are organized into five parts and cover topics such as basic methods of lipid isolation and analysis; mass spectrometry and NMR analysis; lipid isolation and analysis from plant tissues, cell compartments and organelles; lipid signaling, lipid-protein interactions, and imaging; and lipid databases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Plant Lipids: Methods and Protocols is a valuable guide for experienced researchers and undergraduate, graduate, and Ph.D. students. This book is also an excellent resource for novice scientists with little to no experience in lipid experiments who are interested in approaching this field experimentally.




Advances in Lipid Methodology


Book Description

This is the fourth volume of an occasional series of review volumes dealing with aspects of lipid methodology. As with the first three volumes, topics have been selected that have been developing rapidly in recent years and have some importance to lipid analysis. The authors are all leading international experts.Topics covered include: analysis of plant lipoxygenase metabolites, preparative high-performance liquid chromatography of lipids, structural analysis of fatty acids, and analysis of stable isotopes in lipids, among others.




Food Analysis Laboratory Manual


Book Description

This second edition laboratory manual was written to accompany Food Analysis, Fourth Edition, ISBN 978-1-4419-1477-4, by the same author. The 21 laboratory exercises in the manual cover 20 of the 32 chapters in the textbook. Many of the laboratory exercises have multiple sections to cover several methods of analysis for a particular food component of characteristic. Most of the laboratory exercises include the following: introduction, reading assignment, objective, principle of method, chemicals, reagents, precautions and waste disposal, supplies, equipment, procedure, data and calculations, questions, and references. This laboratory manual is ideal for the laboratory portion of undergraduate courses in food analysis.




Lipid Analysis in Oils and Fats


Book Description

This book focuses on the developments in the field of lipid analysis, providing an up-to-date review of the analytical techniques available to chemists and technologists to identify complex molecules. The requisite theoretical background will be provided for individual techniques, together with their strengths and weaknesses, and a guide to the enormous range of commercial applications. It will be an invaluable reference source to all sectors of the oils and fats industry where accurate labeling of foods, food contamination and adulteration are issues of increasing interest and concern.




Encyclopedia of Lipidomics


Book Description

The Encyclopedia of Lipidomics will present a complete overview of the field from fundamentals to new discoveries and from concepts, theories and experimental techniques to clinical and industrial applications. The book will develop with the active involvement of a strong editorial board comprised of leaders from the field. The Encyclopedia of Lipidomics intends to be a comprehensive reference resource serving to bridge the gap between clinical and basic science investigators and provide authoritative and digested information to students, scientists as well as non specialists. The book will have an edge over protocol type of works and databases in terms of having wider coverage through rigorous essays on terms, concepts, experimental and analytical techniques, applications and new challenges. Since lipidomics has strong linkages with several of the other biomedical and life sciences disciplines, the proposed encyclopedia will have a wide audience including graduate students, researchers and different levels of scientists in biomedicine, cellular and molecular biology, bioengineering, physiological and biochemistry, and pharmacology across both academia and industry.




Basic Gas Chromatography


Book Description

The New Edition of the Well-Regarded Handbook on Gas Chromatography Since the publication of the highly successful first edition of Basic Gas Chromatography, the practice of chromatography has undergone several notable developments. Basic Gas Chromatography, Second Edition covers the latest in the field, giving readers the most up-to-date guide available, while maintaining the first edition's practical, applied approach to the subject and its accessibility to a wide range of readers. The text provides comprehensive coverage of basic topics in the field, such as stationary phases, packed columns and inlets, capillary columns and inlets, detectors, and qualitative and quantitative analysis. At the same time, the coverage also features key additions and updated topics including: Gas chromatography-mass spectrometry (GC-MS) Sampling methods Multidimensional gas chromatography Fast gas chromatography Gas chromatography analysis of nonvolatile compounds Inverse gas chromatography and pyrolysis gas chromatography Along with these new and updated topics, the references, resources, and Web sites in Basic Gas Chromatography have been revised to reflect the state of the field. Concise and fundamental in its coverage, Basic Gas Chromatography, Second Edition remains the standard handbook for everyone from undergraduates studying analytical chemistry to working industrial chemists.




Charged Aerosol Detection for Liquid Chromatography and Related Separation Techniques


Book Description

The first book devoted exclusively to a highly popular, relatively new detection technique Charged Aerosol Detection for Liquid Chromatography and Related Separation Techniques presents a comprehensive review of CAD theory, describes its advantages and limitations, and offers extremely well-informed recommendations for its practical use. Using numerous real-world examples based on contributors’ professional experiences, it provides priceless insights into the actual and potential applications of CAD across a wide range of industries. Charged aerosol detection can be combined with a variety of separation techniques and in numerous configurations. While it has been widely adapted for an array of industrial and research applications with great success, it is still a relatively new technique, and its fundamental performance characteristics are not yet fully understood. This book is intended as a tool for scientists seeking to identify the most effective and efficient uses of charged aerosol detection for a given application. Moving naturally from basic to advanced topics, the author relates fundamental principles, practical uses, and applications across a range of industrial settings, including pharmaceuticals, petrochemicals, biotech, and more. Offers timely, authoritative coverage of the theory, experimental techniques, and end-user applications of charged aerosol detection Includes contributions from experts from various fields of applications who explore CAD’s advantages over traditional HPLC techniques, as well its limitations Provides a current theoretical and practical understanding of CAD, derived from authorities on aerosol technology and separation sciences Features numerous real-world examples that help relate fundamental properties and general operational variables of CAD to its performance in a variety of conditions Charged Aerosol Detection for Liquid Chromatography and Related Separation Techniques is a valuable resource for scientists who use chromatographic techniques in academic research and across an array of industrial settings, including the biopharmaceutical, biotechnology, biofuel, chemical, environmental, and food and beverage industries, among others.




Advances in Gas Chromatography


Book Description

For decades gas chromatography has been and will remain an irreplaceable analytical technique in many research areas for both quantitative analysis and qualitative characterization/identification, which is still supplementary with HPLC. This book highlights a few areas where significant advances have been reported recently and/or a revisit of basic concepts is deserved. It provides an overview of instrumental developments, frontline and modern research as well as practical industrial applications. The topics include GC-based metabolomics in biomedical, plant and microbial research, natural products as well as characterization of aging of synthetic materials and industrial monitoring, which are contributions of several experts from different disciplines. It also contains best hand-on practices of sample preparation (derivatization) and data processing in daily research. This book is recommended to both basic and experienced researchers in gas chromatography.