Author : Peter Hinterdorfer
Publisher : Springer Science & Business Media
Page : 634 pages
File Size : 34,4 MB
Release : 2009-12-24
Category : Science
ISBN : 0387764976
This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells.
Author : Chris Gell
Publisher : OUP Oxford
Page : 278 pages
File Size : 27,66 MB
Release : 2006-08-17
Category : Science
ISBN : 9780198529422
This book is aimed at providing a practical introduction to single molecule fluorescence experiments, the analysis of the data, and applications of the techniques to the study of biological structure and function. The techniques have wide applications in biology, and underpin some aspects of nanotechnology and quantum information processing.
Author : Markus Sauer
Publisher : John Wiley & Sons
Page : 425 pages
File Size : 30,83 MB
Release : 2010-12-23
Category : Science
ISBN : 3527633529
Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging. Examples show how each technique can help in obtaining detailed and refined information from individual molecular systems.
Author : Chris Gell
Publisher : OUP Oxford
Page : 278 pages
File Size : 44,70 MB
Release : 2006-08-17
Category : Science
ISBN : 0191523852
Analytical measurements at the single molecule level under ambient conditions have become almost routine in the past few years. The application of this technology to fundamental studies of heterogeneity in biomolecular structure and dynamics, chemical and biological reaction kinetics, and photophysics provides a rich playground for molecular scientists. The potential use of single molecule detection for nanotechnology and quantum information processing is a new and almost unexplored area. This handbook is intended for those interested in a practical introduction to single molecule investigations using fluorescence techniques and places special emphasis on the practicalities of achieving single molecule resolution, analysing the resulting data and exploration of the applications in biophysics. It is ideal for graduate research students and others embarking on work in this exciting field.
Author : Yuri L. Lyubchenko
Publisher : CRC Press
Page : 407 pages
File Size : 32,44 MB
Release : 2017-11-22
Category : Science
ISBN : 1351333097
This book gives an accessible, detailed overview on techniques of single molecule biophysics (SMB), showing how they are applied to numerous biological problems associated with understanding the molecular mechanisms of DNA replication, transcription, and translation, as well as functioning of molecular machines. It covers major single molecule imaging and probing techniques, highlighting key strengths and limitations of each method using recent examples. The chapters begin with a discussion of single molecule fluorescence techniques followed by an overview of the atomic force microscope and its use for direct time-lapse visualization of dynamics of molecular complexes at the nanoscale, as well as applications in measurements of interactions between molecules and mechanical properties of isolated molecules and their complexes. The next chapters address magnetic tweezers and optical tweezers, including instrumentation, fundamentals of operation, and applications. A final chapter turns to nanopore transport and nanopore-based DNA sequencing technology that will play a major role in next-generation genomics and healthcare applications.
Author : Keiko Hirose
Publisher : CRC Press
Page : 436 pages
File Size : 16,7 MB
Release : 2019-05-28
Category : Science
ISBN : 0429667434
Dyneins are molecular motors that are involved in various cellular processes, such as cilia and flagella motility, vesicular transport, and mitosis. Since the first edition of this book was published in 2012, there has been a significant breakthrough: the crystal structures of the motor domains of cytoplasmic dynein have been solved and the previously unknown details of this huge and complex molecule have been unveiled. This new edition contains 14 chapters written by researchers in the US, Europe, and Asia, including 3 new chapters that incorporate new fields. The other chapters have also been substantially updated. Compared with the earlier edition, this book focuses more on the motile mechanisms of dynein, especially by biophysical methods such as cryo-EM, X-ray crystallography, and single-molecule nanometry. It is a major handbook for frontline researchers as well as for advanced students studying cell biology, molecular biology, biochemistry, biophysics, and structural biology.
Author : Susanta K Sarkar
Publisher : Morgan & Claypool Publishers
Page : 64 pages
File Size : 36,94 MB
Release : 2016-04-01
Category : Science
ISBN : 1681741806
This is an overview of single molecule physics, the study of both equilibrium and non-equilibrium properties at the single molecule level. It begins with an introduction to this fascinating science and includes a chapter on how to build the most popular instrument for single molecule biophysics, the total internal reflection fluorescence (TIRF) microscope. It concludes with the Poisson process approach to statistical mechanics, explaining how to relate the process to diverse areas and see how data analysis and error bars are integral parts of science.
Author : Tamiki Komatsuzaki
Publisher : Wiley
Page : 512 pages
File Size : 44,24 MB
Release : 2011-11-16
Category : Science
ISBN : 9781118131398
Discover the experimental and theoretical developments in optical single-molecule spectroscopy that are changing the ways we think about molecules and atoms The Advances in Chemical Physics series provides the chemical physics field with a forum for critical, authoritative evaluations of advances in every area of the discipline. This latest volume explores the advent of optical single-molecule spectroscopy, and how atomic force microscopy has empowered novel experiments on individual biomolecules, opening up new frontiers in molecular and cell biology and leading to new theoretical approaches and insights. Organized into two parts—one experimental, the other theoretical—this volume explores advances across the field of single-molecule biophysics, presenting new perspectives on the theoretical properties of atoms and molecules. Single-molecule experiments have provided fresh perspectives on questions such as how proteins fold to specific conformations from highly heterogeneous structures, how signal transductions take place on the molecular level, and how proteins behave in membranes and living cells.This volume is designed to further contribute to the rapid development of single-molecule biophysics research. Filled with cutting-edge research reported in a cohesive manner not found elsewhere in the literature, each volume of the Advances in Chemical Physics series serves as the perfect supplement to any advanced graduate class devoted to the study of chemical physics.
Author : Krishnarao Appasani
Publisher :
Page : 172 pages
File Size : 37,30 MB
Release : 2022-05-11
Category : Medical
ISBN : 110853449X
A comprehensive volume that brings together authoritative overviews of single molecule science techniques from a biological perspective.
Author : Henrik G. Bohr
Publisher : Wiley-VCH
Page : 1074 pages
File Size : 35,55 MB
Release : 2009-05-26
Category : Science
ISBN : 9783527407026
This handbook and reference condenses the biophysics and biomedical contents of the renowned Encyclopedia of Applied Physics in one handy volume. Twenty-eight carefully written overview articles cover the latest research, including single molecule spectroscopy, biosensors and cellular biomechanics. Readers benefit from concise summaries of the fundamentals, methods and applications, backed by detailed tables of contents for quick access, and glossaries of terms, as well as detailed lists of references and further reading. For libraries and R&D teams in academia and industry.
