Book Description
Introduction to the general principles of HPLC; HPLC instrumentation; Mobile phases; Detection methods; Separation of free amino acids; Resolution of amino acids as diastereomeric derivatives.
Author : William S. Hancock
Publisher : CRC-Press
Page : 514 pages
File Size : 43,44 MB
Release : 1984-07-02
Category : Science
ISBN : 9780849335105
Introduction to the general principles of HPLC; HPLC instrumentation; Mobile phases; Detection methods; Separation of free amino acids; Resolution of amino acids as diastereomeric derivatives.
Author : William S. Hancock
Publisher : CRC-Press
Page : 544 pages
File Size : 41,51 MB
Release : 1984-08-14
Category : Science
ISBN : 9780849335112
All areas of high performance liquid chromatography (HPLC) relevant to the separation of amino acid and polypeptide samples are described in these comprehensive volumes. Eighty-four chapters cover the application of HPLC to research in the life sciences. Both basic information and specific details are presented on such areas as commercially available HPLC columns, ligand-exchange chromatography, peptide hormone separations, peptide antibiotic separations and drug-protein binding. Practical suggestions are given to help you avoid pitfalls which can exist in the analysis of biological materials.
Author : Taylor & Francis Group
Publisher :
Page : pages
File Size : 44,25 MB
Release : 2018-09-30
Category :
ISBN : 9781315891972
Author : Colin T. Mant
Publisher : CRC Press
Page : 960 pages
File Size : 14,75 MB
Release : 1991-07-24
Category : Science
ISBN : 9780849365492
This book consists of a series of 82 precise, easy-to-read articles by internationally renowned scientists and emphasizes the practical approach to HPLC with minimal theory, although the underlying principles for peptide and protein separations are clearly expressed. All of the major modes of microbore, ultrafast and analytical HPLC are discussed, including size-exclusion, ion-exchange, reversed-phase, hydrophobic interaction, and affinity and immunoaffinity chromatography. A section on preparative HPLC, including displacement techniques, is also presented. Problem-solving approaches to the separation of various classes of biologically active peptides and proteins are thoroughly explored, while the importance of peptide standards for monitoring column performance and for optimizing separation conditions is emphasized. Several articles focus on the choice of the correct detection method (electrochemical, UV, fluorescence), as well as the need for a proper knowledge of approaches to column and instrument maintenance and trouble-shooting. A section on predictive approaches deals with both computer simulation of peptide separations and peptide structure. The book also includes complementary techniques to HPLC, as well as other useful applications of HPLC. It enables both novice and experienced chromatographers to realize the full potential of this extremely powerful technique, in the process making an important contribution to scientific literature.
Author : Paul T. Matsudaira
Publisher : Gulf Professional Publishing
Page : 212 pages
File Size : 14,72 MB
Release : 1993-05-20
Category : Science
ISBN : 9780124802827
Why a Second Edition? The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. Mass spectrophotometric characterization of proteins and peptides N-terminal sequencing of proteins with blocked N termini Internal amino acid sequence analysis after protease digestion in-gel and on-blot Improved microscale peptide purification methods Computer analysis of protein sequences New protocols tested and refined through everyday use in authors' laboratories Updated reference chapter covering all aspects of protein microsequencing
Author : J. Giacometti
Publisher : Elsevier Inc. Chapters
Page : 57 pages
File Size : 40,90 MB
Release : 2013-01-08
Category : Science
ISBN : 0128071125
Since the introduction of suitable, highly porous supports and later nonporous and monolithic supports, chromatography has been an indispensible method for protein separations on the analytical and preparative scales. The most frequently used methods for protein chromatography are ion exchange, reversed phase, hydrophobic interaction chromatography, chromatography on hydroxyapatite and different types of affinity, and pseudo-affinity chromatography. Because of the use of organic solvents during the separation in reversed-phase chromatography, denaturation and loss of biological activity frequently occurs, and this method is less suitable for separation of biologically active, therapeutic proteins. Chromatography on monolithic supports and chromatography in the displacement mode offer additional opportunities for fast, highly effective separation of proteins on both the analytical and preparative scales.
Author : William S. Hancock
Publisher :
Page : 489 pages
File Size : 37,20 MB
Release : 1984
Category :
ISBN :
Author : William S. Hancock
Publisher :
Page : pages
File Size : 26,52 MB
Release : 1984
Category : Amino acids
ISBN :
Author : Chemical Rubber Company (Cleveland, Ohio)
Publisher :
Page : pages
File Size : 21,36 MB
Release : 1989
Category :
ISBN :
Author : S. Blackburn
Publisher : CRC Press
Page : 427 pages
File Size : 12,77 MB
Release : 2021-05-30
Category : Science
ISBN : 1000443337
An in-depth analysis of chromatography literature and procedures since 1981 is presented in this publication. Featured is a compre-hensive range of tables relating to the chromatographic separation and determination of amino acids, amines, and their derivatives. Methods of sample preparation and derivatization and methods of detection are described. Included are techniques for the liquid and gas chro-matographic separation of free amino acids and their derivatives, including o-phthalaldehyde, dansyl, and phenylthiolaydantoin deriva-tives. The separation of amino acid enantiomers is also described. This book will be invaluable to chemists, biochemists, and analysts involved in the separation and determination of amino acids or amines and their derivatives.