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Hedgehog Signaling Protocols

Author : Jamila I. Horabin

Publisher : Springer Science & Business Media

Page : 267 pages

File Size : 14,90 MB

Release : 2007-09-13

Category : Medical

ISBN : 158829692X

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Book Description

In both vertebrates and invertebrates, Hedgehog signaling regulates the development of the tissues and structures of the body. In addition, many cancers and diseases arise as a result of misregulation of the pathway, which makes it an incredibly important system to understand. This volume examines various methods used in the analysis of the Hedgehog signaling pathway, bringing together different lines of analyses into one accessible and comprehensive text. This volume provides a single source for investigators, presenting several different experimental approaches, which are all varied and broad in nature, as well as procedures for a variety of different model systems. Researchers will find this volume to be an invaluble tool as they work towards deeper understanding of the underlying molecular events that the Hedgehog signaling pathway regulates.



Hedgehog Signaling

Author : Xiaochun Li

Publisher : Humana

Page : 0 pages

File Size : 10,78 MB

Release : 2022-10-11

Category : Science

ISBN : 9781071617038

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Book Description

This detailed book explores the technical breakthroughs with biophysical and cell biological approaches that have advanced the study of molecular mechanisms underlying the generation and transduction of the hedgehog (HH) signal, indicating the important role of sterols in this pathway. Within its pages, the volume examines techniques involving several key protein components in this pathway, including HH, the signaling ligand; Dispatched, a HH secretion regulator; Patched, the receptor of HH; Smoothened, the signal transducer used to transduce the HH signal across the plasma membrane; and GLI, the transcription factor to turn on HH target genes. Written in the highly successful Methods in Molecular Biology series format, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Hedgehog Signaling: Methods and Protocols provides researchers in clinical and basic science with a fresh and reliable reference for analyzing HH signal transduction through cell biological, biochemical, and biophysical approaches.



Cell Signalling

Author : John Hancock

Publisher : Oxford University Press, USA

Page : 364 pages

File Size : 44,60 MB

Release : 2010-01-21

Category : Education

ISBN : 0199232105

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Book Description

Signalling within and between cells is an essential part of many biological processes, from the development of the body to the activity of our immune system. Cell Signalling, Third Edition, presents a carefully structured introduction to this intricate subject, introducing those conserved features that underlie many different extra- and intracellular signalling systems. Starting with an overview of cell signalling and highlighting its importance in many biological systems, the book goes on to explore the key components of extracellular and intracellular signalling mechanisms before examining how these components come together to create signalling pathways, which are so crucial to the survival of many living organisms. The text is enhanced by two-color artwork and 3-D protein models. A Companion Website provides resources for students and instructors.



Regenerative Medicine - from Protocol to Patient

Author : Gustav Steinhoff

Publisher : Springer

Page : 378 pages

File Size : 35,33 MB

Release : 2016-04-25

Category : Science

ISBN : 3319275836

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Book Description

Regenerative medicine is the main field of groundbreaking medical development and therapy using knowledge from developmental and stem cell biology, as well as advanced molecular and cellular techniques. This collection of volumes on Regenerative Medicine: From Protocol to Patient, aims to explain the scientific knowledge and emerging technology, as well as the clinical application in different organ systems and diseases. International leading experts from all over the world describe the latest scientific and clinical knowledge of the field of regenerative medicine. The process of translating science of laboratory protocols into therapies is explained in sections on regulatory, ethical and industrial issues. This collection is organized into five volumes: (1) Biology of Tissue Regeneration, (2) Stem Cell Science and Technology, (3) Tissue Engineering, Biomaterials and Nanotechnology, (4) Regenerative Therapies I, and (5) Regenerative Therapies II. The textbook gives the student, the researcher, the health care professional, the physician and the patient a complete survey on the current scientific basis, therapeutical protocols, clinical translation and practiced therapies in regenerative medicine. Volume 1 contains eleven chapters addressing the latest basic science knowledge on the “Biology of Tissue Regeneration”. The principles of cell regeneration control by extracellular matrix and the biology of stem cell niches are explained. Depicted are the principles of molecular mechanisms controlling asymmetric cell division, stem cell differentiation, developmental and regenerative biology, epigenetic and genetic control as well as mathematical modelling for cell fate prediction. Regenerative biology of stem cells in the central nervous and cardiovascular systems leading to complex tissue regeneration in the model species axolotl and zebrafish, as well as the impact of immune signalling on nuclear reprogramming are outlined. These up to date accounts gives the readers advanced insights into the biological principles of the regenerative processes in stem cells, tissues and organisms.



The Analysis of Gene Expression Data

Author : Giovanni Parmigiani

Publisher : Springer Science & Business Media

Page : 511 pages

File Size : 28,34 MB

Release : 2006-04-11

Category : Medical

ISBN : 0387216790

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Book Description

This book presents practical approaches for the analysis of data from gene expression micro-arrays. It describes the conceptual and methodological underpinning for a statistical tool and its implementation in software. The book includes coverage of various packages that are part of the Bioconductor project and several related R tools. The materials presented cover a range of software tools designed for varied audiences.



Pichia Protocols

Author : James M Cregg

Publisher : Springer Science & Business Media

Page : 553 pages

File Size : 16,1 MB

Release : 2007-08-08

Category : Science

ISBN : 1588294293

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Book Description

This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI). Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system. This volume fully updates and expands upon the first edition.



Adhesion Protein Protocols

Author : Amanda S. Coutts

Publisher : Springer Science & Business Media

Page : 237 pages

File Size : 34,10 MB

Release : 2008-02-03

Category : Science

ISBN : 1597453536

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Book Description

The second edition of Adhesion Protein Protocols combines traditional techniques with cutting-edge and novel techniques that can be adapted easily to different molecules and cell types. The topics discussed include novel techniques for studying cell-cell adhesion, neutrophil chemotaxis, in vitro assays used to study leukocyte migration through monolayers of cultured endothelial cells, and novel techniques to purify pseudopodia from migratory cells. The protocols discussed in this volume are suitable for both novice and expert scientists, who will gain further insight into the complex and incompletely understood processes involved in cellular adhesion.



Cryopreservation and Freeze-Drying Protocols

Author : John G. Day

Publisher : Springer Science & Business Media

Page : 703 pages

File Size : 45,13 MB

Release : 2007-06-05

Category : Science

ISBN : 1588293777

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Book Description

In addition to outlining the fundamental principles associated with the conservation of biological resources, freeze-drying and cryopreservation, this text is a compilation of cryptopreservation and freeze-drying methodologies applicable to different biological materiels, developed by expert laboratories.



In Vitro Transcription and Translation Protocols

Author : Guido Grandi

Publisher : Springer Science & Business Media

Page : 568 pages

File Size : 48,41 MB

Release : 2007-05-03

Category : Medical

ISBN : 1588295583

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Book Description

This book is a highly anticipated update of the previous edition. It provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems. It has been completely updated with new chapters and topics.



Bacterial Pathogenesis

Author : Frank R. DeLeo

Publisher : Springer Science & Business Media

Page : 314 pages

File Size : 43,56 MB

Release : 2008-01-31

Category : Medical

ISBN : 1588297403

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Book Description

Bacterial infections affect world health today as a leading cause of morbidity and mortality. This book presents in-depth methods and state-of-the-art protocols for investigating specific mechanisms of pathogenesis for a wide range of bacteria. Written by experts in the field, this invaluable collection includes protocols to study host-pathogen interactions, animal models of infection, and novel approaches to identifying therapeutic targets designed to control infections.