Experimental Techniques in Host-Plant Resistance


Book Description

The earliest land-plants evolved around 450 million years ago from aquatic plants devoid of vascular systems. The diversification of flowering plants (angiosperms) during the Cretaceous period is associated with speciation in insects. Early insect herbivores were mandibulate, but the evolution of vascular plants led to the co-evolution of other forms of herbivory, such as leaf feeding, sap-sucking, leaf mining, tissue borer, gall forming and nectar-feeding. Plant defense against biotic stress is an adaptive evolution by plants to increase their fitness. Plants use a variety of strategies to defend against damage caused by herbivores. Plant defense mechanisms are either inbuilt or induced. Inbuilt mechanisms are always present within the plant, while induced defenses are produced or mobilized to the site where a plant is injured. Induced defense mechanisms include morphological, physiological changes and production of secondary metabolites. Host plant resistance (HPR) is one of the eco-friendly methods of pest management. It protects the crop by making it less suitable or tolerant to the pest. While books on theoretical aspects of HPR are available, an exclusive book on the practical aspects is lacking. There is a wide gap between the theory and the experimental procedures required for conducting studies on plant resistance for the post graduate students and young researchers. A dire need for a book on practical aspects was strongly felt. Initially a practical manual was prepared which eventually evolved into the present book. We hope this book provides information on major aspects of screening crop germplasm, sampling techniques, genetic and biochemical basis of HPR, behavioural studies on pheromone and plant volatiles, and some of the recent approaches in HPR. Further, the references provide the scientific articles and books as additional information to readers and workers alike.




Molecular Host Plant Resistance to Pests


Book Description

Molecular Host Plant Resistance to Pests examines environmentally safe and integrated techniques for effective pest management. Offering more than 1500 references for further exploration of the topic, this reference details the bioactivity, biosynthetic pathways, mechanisms of action, and genetic regulation for improved methods of crop protection a




Managing Global Genetic Resources


Book Description

This anchor volume to the series Managing Global Genetic Resources examines the structure that underlies efforts to preserve genetic material, including the worldwide network of genetic collections; the role of biotechnology; and a host of issues that surround management and use. Among the topics explored are in situ versus ex situ conservation, management of very large collections of genetic material, problems of quarantine, the controversy over ownership or copyright of genetic material, and more.




Plant Resistance to Arthropods


Book Description

Arthropod resistant crops reduce pesticide pollution, alleviate hunger and improve human nutrition. This book reviews new information on environmental advantages of plant resistance, transgenic resistance, molecular bases of resistance, and use of molecular markers to map resistance genes.




Handbook of Maize: Its Biology


Book Description

Handbook of Maize: Its Biology centers on the past, present and future of maize as a model for plant science research and crop improvement. The book includes brief, focused chapters from the foremost maize experts and features a succinct collection of informative images representing the maize germplasm collection.




The Tomato Crop


Book Description

The tomato is commercially important throughout the world both for the fresh fruit market and the processed food industries. It is grown in a wide range of climates in the field, under protection in plastic greenhouses and in heated glasshouses. Genetic, physiological and pathological investigations frequently adopt the tomato plant as a convenient subject. Hitherto, much of the information on tomatoes has been fragmented: tomatoes grown in the field and under protection have been considered separately and the more fundamental findings from research have often failed to reach those involved directly or indirectly in commercial crop production. Similarly, the research scientist is often unaware of the problems of commercial crop production and the possible relevance of his work to the crop. This book is an attempt to rectify that situation. By giving a thorough scientific review of all factors influencing tomato production systems, it is hoped that this book will prove useful to students, researchers and commercial producers alike. It gives the basis for the develop ment of improved cultivars, the formulation of strategies for managing pest, disease and disorder problems and the production of high yields of good quality fruit as well as suggesting important areas for scientific initiatives. The extensive bibliographies provide a comprehensive database for tomato researchers. Such a vast subject could not be covered with authority by anyone author.




Principles of Host-plant Resistance to Insect Pests


Book Description

Introduction; Insect-plant interaction; Host-plant selection in Phytophagous insects; Mechanisms of resistance; Biochemistry of resistance; Factors affecting expression of resistance; Resistance programme; Genetics of resistance; Plant resistance in pest management.










Methods in Virology


Book Description

Methods in Virology, Volume VII focuses on the methods used in virology, including radioimmunoassays, microscopy, hybridization, and mutagenesis. The selection first elaborates on monoclonal antibody techniques applied to viruses; competition radioimmunoassays for characterization of antibody reactions to viral antigens; and enzyme immunosorbent assays in plant virology. Discussions focus on the principles of enzyme immunosorbent assay, choice of enzyme and preparation of conjugate, determination of immunoglobulin class, and maintenance and specificity testing of hybridomas. The text then elaborates on electron microscopy for the identification of plant viruses in in vitro preparations and cloning and expression of viral antigens in Escherichia coli and other microorganisms, including influenza virus, expression of foreign coding sequences in Escherichia coli, hepatitis B virus, electron microscope, immunoelectron microscopy, and imaging of nucleic acids. The manuscript takes a look at the detection and characterization of subgenomic RNA in plant viruses; exploring the gene organization of baculoviruses; and spot hybridization for detection of viroids and viruses. Topics include application to viral diseases, mapping mutuations of baculoviruses, transcriptional mapping of baculovirus genomes, and genetic mapping by blot hybridization. The selection is a valuable source of information for researchers interested in the methods employed in virology.