Author : J. Tampion
Publisher : Cambridge University Press
Page : 268 pages
File Size : 33,81 MB
Release : 1987-12-10
Category : Medical
ISBN : 9780521255561
This 1987 book gives a coherent overview of preparation and uses of immobilized enzymes.
Author : Ichiro Chibata
Publisher : Elsevier
Page : 372 pages
File Size : 16,28 MB
Release : 2014-06-28
Category : Science
ISBN : 1483215865
Immobilized Microbial Cells, Volume 4 provides an overview of the methods of immobilization, applications, and ways of utilizing immobilized microbial cells and subcellular organelles and chloroplasts as biocatalysts. This volume is comprised of seven chapters. It begins with the historical background of immobilized cell research. Subsequent chapters focus on the methods of immobilization and applications of immobilized microbial cells, living cells, and organelles. The last two chapters discuss gas production of immobilized cells for energy generation and the chemical engineering analysis of immobilized-cell systems. The book will be of great use to chemists and chemical engineers.
Author : Jose M. Guisan
Publisher : Humana
Page : 0 pages
File Size : 46,57 MB
Release : 2016-08-27
Category : Science
ISBN : 9781493959884
For most of industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used for very long periods of time. From this point of view, immobilization, simplicity and stabilization have to be strongly related concepts. The third edition of Immobilization of Enzymes and Cells expands upon and updates the previous editions with current, detailed protocols for immobilization. With new chapters on protocols for immobilization of enzymes and cells which may be useful to greatly improve the functional properties of enzymes and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immobilization of Enzymes and Cells, Third Edition demonstrates simple and efficient protocols for the preparation, characterization, and utilization of immobilized enzymes and cells.
Author : Jose M. Guisan
Publisher : Humana
Page : 495 pages
File Size : 28,43 MB
Release : 2021-01-29
Category : Science
ISBN : 9781071602171
This fourth edition volume expands on the previous editions with new insights on important aspects to take into accounting when immobilizing enzymes and cells, illustrating outstanding examples that support those aspects, and exploring ways to fabricate and characterize heterogeneous biocatalysts including both immobilized enzymes and cells. The transformation of soluble and usually instable enzymes into heterogeneous and highly stable biocatalysts is strongly emphasized. The chapters in this book cover topics such as the importance of enzyme orientation on the support surface; application and characterization of immobilized enzymes; different functionalization chemistries for the modulation of the immobilized enzyme properties; co-immobilization of multi-enzyme systems; new analytical techniques for the characterization of heterogeneous biocatalysts; protocols for cell entrapment in alginate; preparation and characterization of biofilms; and cell encapsulation technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Immobilization of Enzymes and Cells: Methods and Protocols, Fourth Edition is a valuable resource for researchers interested in expanding their knowledge of this developing field.
Author : R. D. Tyagi
Publisher : CRC Press
Page : 294 pages
File Size : 12,98 MB
Release : 1990-05-23
Category : Technology & Engineering
ISBN : 9780849351761
The purpose of this state-of-the-art publication is to provide up-to-date and pertinent scientific information concerning immobilized cell processes for treatment of wastewater. It comprehensively reviews and examines essential data on the feasibility of various immobilization methods, with special reference to wastewater treatment. It also discusses methods of identification of structure and composition of microbial aggregates and analytical methods for the estimation of biomass in the presence of carriers. This work reports the novel process of immobilized microalgae and cyanobacteria for wastewater treatment, while highlighting their future prospects. Additionally, it critically reviews the various fixed film bioreactor configurations. This book is a must for all engineers, planners, scientists, students and sewage treatment plant operators.
Author : Viktor Nedovic
Publisher : Springer Science & Business Media
Page : 542 pages
File Size : 30,99 MB
Release : 2013-04-17
Category : Science
ISBN : 9401716382
Cell Immobilisation Biotechnology Biotechnology is divided into two volumes. The first volume is dedicated to fundamental aspects of cell immobilisation while the second volume deals with the diverse applications of this technology. The first volume, Fundamentals of Cell Immobilisation Biotechnology, comprises 26 chapters arranged into four parts: Materials for cell immobilisation/encapsulation, Methods and technologies for cell immobilisation/encapsulation, Carrier characterisation and bioreactor design, and Physiology of immobilised cells: techniques and mathematical modelling.
Author : José M. Guisán
Publisher : Springer Science & Business Media
Page : 447 pages
File Size : 36,61 MB
Release : 2008-02-05
Category : Science
ISBN : 1597450537
Enzymes and whole cells are able to catalyze the most complex chemical processes under the most benign experimental and environmental conditions. In this way, enzymes and cells could be excellent catalysts for a much more sustainable chemical industry. However, enzymes and cells also have some limitations for nonbiological applications: fine chemistry, food chemistry, analysis, therapeutics, and so on. Enzymes and cells may be unstable, difficult to handle under nonconventional conditions, poorly selective toward synthetic substrates, and so forth. From this point of view, the transformation—from the laboratory to industry—of chemical processes catalyzed by enzymes and cells may be one of the most complex and exciting goals in biotechnology. For many industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used over very long periods of time. From this point of view, immobilization, simplicity, and stabilization have to be strongly related concepts. Over the last 30 years, a number of protocols for the immobilization of cells and enzymes have been reported in scientific literature. However, only very few protocols are simple and useful enough to greatly improve the functional properties of enzymes and cells, activity, stability, selectivity, and related properties.
Author : Allen I. Laskin
Publisher : Butterworth-Heinemann
Page : 344 pages
File Size : 48,70 MB
Release : 1985
Category : Science
ISBN :
Author : Winfried Hartmeier
Publisher : Springer Science & Business Media
Page : 217 pages
File Size : 30,41 MB
Release : 2012-12-06
Category : Science
ISBN : 3642733646
Biotechnological processes often require the use of immobilized biocatalysts. This translation of the most successful German edition introduces the theoretical background of this rapidly growing field followed by a detailed description of the various techniques of immobilizing, characterizing, and using biocatalysts. The comprehensive discussion of possible applications in industrial processes and in basic research results in a practical guide for everyone involved in these techniques. Furthermore, some selected experiments are added in order to facilitate the understanding of the theoretical reports.
Author : Gordon Bickerstaff
Publisher : Humana Press
Page : 367 pages
File Size : 12,17 MB
Release : 1996-12-01
Category : Science
ISBN : 9780896033863
Immobihzatron of enzymes, cells, and organelles has expanded greatly in the past 30 years as the advantages of immobilization have been evaluated and utilized in analyttcal, biotransformation, and medical applications. A c- sequence of this explosion of technology IS that there is now a bewildering array of permutations for the immobilization of biological material. The p- pose of Immobilization of Enzymes and Cells is to provrde a basic reference tool for all academic and industrial research workers seeking to start or expand the use of mnnobilization techniques in their work. The book does not aim to provide comprehensive coverage of the vast range of methods available, but will serve as a launch pad for potential users of immobilization techniques. One reason for the vast expanse of mmrobilization technology lies m the subject material to be immobilized. Biological catalysts (enzymes, organelles, and cells) have a high degree of individual variability, and although many tmmobilization techniques have wide applicability, tt is imposstble for one or even a few methods to cater to the great diversity of requirements inherent in biological material. This is especially so when the atm is to produce an op- mum system m which the immobihzed biocatalyst will function at high levels of efficrency, stability, and so on.
