Measuring Plant-associated Nitrogen Fixation in Agricultural Systems


Book Description

Biological nitrogen fixation. Why, when an how to measure nitrogen fixation. Analysis of nitrogen. Nitrogen Balance Method. Nitrogen Difference method. Ureide (N solute) metode. N-isotopic methods. N-abundance method. N isotopic ditution method. prcaution whenquantifying N2 fixation associated with no-nodulatin plants (associative N2 fixation). Assays of nitrogenase activity.




Methods for Evaluating Biological Nitrogen Fixation


Book Description

The cultivation of diazotrophic microorganisms. Measurement of nitrogen fixation by direct means. Measurement of nitrogen fixation by indirect means. Methods for legumes in glasshouses and controlled environment cabinets. Non-legumes nodule systems. Methods for studying nitrogenase. Methods for studying enzymes involved in metabolism related to nitrogenase. Preparation and experimental use of leghaemoglobin. Methods for identifying strains of diazotrophs. Genetic studies with diazotrophs. Experiments with crop and pasture legumes: principles and practice. Production and quality control of legume inoculants. Forage grasses and grain crops. Nitrogen fixation in natural plant communities and soils. Sytems involving blue-green algae (cyanobacteria).




Handbook for Rhizobia


Book Description

Rhizobia are bacteria which inhabit the roots of plants in the pea family and "fix" atmospheric nitrogen for plant growth. They are thus of enormous economic importance internationally and the subject of intense research interest. Handbook for Rhizobia is a monumental book of practical methods for working with these bacteria and their plant hosts. Topics include the general microbiological properties of rhizobia and their identification, their potential as symbionts, methods for inoculating rhizobia onto plants, and molecular genetics methods for Rhizobium in the laboratory. The book will be invaluable to Rhizobium scientists, soil microbiologists, field and laboratory researchers at agricultural research centers, agronomists, and crop scientists.







Biological nitrogen fixation in forest ecosystems: foundations and applications


Book Description

Silvicultural systems and biological nitrogen fixation; Morphology of nitrogen fixers in forest ecosystems; Taxonomy and distribution of non-legume nitrogen-fixing systems; Isolation and culture of nitrogen-fixing organisms; Wheeler, biochemical, physiological and environmental aspects of symbiotic nitrogen fixation; Analysis of nitrogen fixation; Agricultural and horticultural systems: implications for forestry; Nitrogen fixing plants in forest plantation management; Nitrogen fixation in North American forestry: research and application; Application of biological nitrogen fixation in European silviculture; Nitrogen fixation in Southeast Asian forestry: research and practice; Biological nitrogen fixation in forestry: research and practice in Australia and New Zealand.




Technical Handbook on Symbiotic Nitrogen Fixation


Book Description

General information on the symbiotic nitrogen fixation. Isolation, identification and counting of rhizobia. Production of an inoculant and inoculation of legumes. Experiments.




Stable Isotope Techniques in the Study of Biological Processes and Functioning of Ecosystems


Book Description

This book has a very strong practical orientation, telling readers what methodologies are available using stable isotopes, how studies should be designed and executed to maximise effectiveness and incisiveness in terms of data obtained and outcomes in terms of understanding events and processes in plant and ecosystem functioning. The readership is very much aimed toward postgraduates with good knowledge of general biological principles and underlying chemical and physical processes. Pre-existing knowledge of or experience in application and assay of stable isotopes is not required. The stable isotope research field is one of the most rapidly growing areas of ecophysiology and techniques using stable isotopes comprise an ever increasing component of research programs of university postgraduates and a wide range of agencies conducting environmental monitoring and rehabilitation programs. The book is tailor-made for such an audience.




Nitrogen Fixation in Acacias


Book Description

2. The plant; 3. The bacteria; 4. The symbiosis; 5. Enhancement of the symbiosis; 6. Exploitation of the symbiosis; 7. General conclusions and prognosis.




Symbiotic Nitrogen Fixation


Book Description

During the past three decades there has been a large amount of research on biological nitrogen fixation, in part stimulated by increasing world prices of nitrogen-containing fertilizers and environmental concerns. In the last several years, research on plant--microbe interactions, and symbiotic and asymbiotic nitrogen fixation has become truly interdisciplinary in nature, stimulated to some degree by the use of modern genetic techniques. These methodologies have allowed us to make detailed analyses of plant and bacterial genes involved in symbiotic processes and to follow the growth and persistence of the root-nodule bacteria and free-living nitrogen-fixing bacteria in soils. Through the efforts of a large number of researchers we now have a better understanding of the ecology of rhizobia, environmental parameters affecting the infection and nodulation process, the nature of specificity, the biochemistry of host plants and microsymbionts, and chemical signalling between symbiotic partners. This volume gives a summary of current research efforts and knowledge in the field of biological nitrogen fixation. Since the research field is diverse in nature, this book presents a collection of papers in the major research area of physiology and metabolism, genetics, evolution, taxonomy, ecology, and international programs.