Methods for Plant Molecular Biology


Book Description

Methods for Plant Molecular Biology is a collection of articles that focuses on the techniques used in plant molecular biology and genetics. The book discusses the isolation and characterization of nuclear, chloroplast, and mitochondrial nucleic acids and the factors and systems involved in transcription and gene expression. Procedures for the isolation of cell walls, chloroplast membranes, membrane proteins; techniques to carry out plant cell culture and protoplast formation; and methods for gene and organelle transfer are covered as well. Biologists, molecular biologists, botanists, and students will find the book very useful.




Plant Molecular Biology


Book Description

In the preface to the first edition ofthis book, we expressed a conviction that there was a need for a short book that highlighted important advances in the new discipline of plant molecular biology. The rapid development of this topic has been brought about by the recognition of the unique properties of plants in the study of growth and development together with the application of recombinant DNA techniques to tackle these problems. Plant cells contain DNA in nuclei, plastids and mitochondria, and so offer the unique challenge of studying the interaction of three separate genetic systems in a single organism. The molecular approach has provided, in recent years, a wealth of important information about how plants function, and how they interact with bacteria, fungi and viruses. Furthermore, plant development involves the regulation of gene expression in response to intern al and external signals, and plant molecular biology has provided a fundamental insight into how this development is regulated. This is not only of considerable scientific interest, but also has important implications for the production of plants and plant products in agriculture, horticulture and the food industries.




The Molecular Biology of Chloroplasts and Mitochondria in Chlamydomonas


Book Description

Provides a thorough overview of current research with the green alga Chlamydomonas on chloroplast and mitochondrial biogenesis and function, with an emphasis on the assembly and structure-function relationships of the constituents of the photosynthetic apparatus. Contributions emphasize the multidisciplinary nature of current research in photosynthesis, combining molecular genetics, biochemical, biophysical, and physiological approaches. The 36 articles address topics including nuclear genome organization; RNA stability and processing; splicing; translation; protein targeting in the chloroplast; photosystems; pigments; glycerolipids; the ATP synthase; and ferrodoxin and thioredoxin. Further contributions address new measurements methods for photosynthetic activity in vivo; starch biosynthesis; the responses of Chlamydomonas to various stress conditions; nitrogen assimilation; and mitochondrial genetics. Annotation copyrighted by Book News, Inc., Portland, OR




Transgenic Microalgae as Green Cell Factories


Book Description

Microalgae have been largely commercialized as food and feed additives, and their potential as a source of high-added value compounds is well known. Yet, only a few species of microalgae have been genetically transformed with efficiency. A better understanding of the mechanisms that control the regulation of gene expression in eukaryotes is therefore needed. In this book a group of outstanding researchers working on different areas of microalgae biotechnology offer a global vision of the genetic manipulation of microalgae and their applications.




Plant Cell Culture Protocols


Book Description

A comprehensive state-of-the-art collection of the most frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. Two new appendices explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, and list more than 100 very useful internet sites.




The Genus Citrus


Book Description

The Genus Citrus presents the enormous amount of new knowledge that has been generated in recent years on nearly all topics related to citrus. Beginning with an overview of the fundamental principles and understanding of citrus biology and behavior, the book provides a comprehensive view from Citrus evolution to current market importance. Reporting on new insights supported by the elucidation of the citrus genome sequence, it presents groundbreaking theories and fills in previous knowledge gaps. Because citrus is among the most difficult plants to improve through traditional breeding, citrus researchers, institutions and industries must quickly learn to adapt to new developments, knowledge and technologies to address the biological constraints of a unique fruit-tree such as citrus. Despite the challenges of working with citrus, tremendous progress has been made, mostly through advances in molecular biology and genomics. This book is valuable for all those involved with researching and advancing, producing, processing, and delivering citrus products. - Includes the most current research on citrus genomic information - Provides the first detailed description of citrus origin, a new proposal for citrus taxonomy, and a redefinition of the genus Citrus - Details citrus challenges including climate change, global disease impacts, and plant improvement strategies




Chloroplast Biotechnology


Book Description

In Chloroplast Biotechnology: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used in chloroplast molecular biology. Chapters focus on essential background information, applications in tobacco and protocols for plastid transformation in crops and Chlamydomonas and Bryophytes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoidance of known pitfalls. Authoritative and practical, Chloroplast Biotechnology: Methods and Protocols seek to aid scientists who study chloroplast molecular biology as well as those interested in applications in agriculture, industrial biotechnology and healthcare.




Methods in Plant Molecular Biology


Book Description

Methods in Plant Molecular Biology is a lab manual that introduces students to a diversity of molecular techniques needed for experiments with plant cells. Those included have been perfected and are now presented for the first time in a usable and teachable form. Because the manual integrates protein, RNA, and DNA techniques, it will serve students, teachers, and researchers in plant physiology, biophysics, and animal molecular biology who have no previous experience handling recombinant DNA or purified proteins. It can also be used by the established molecular biologist who wishes to utilize the powerful techniques of recombinant DNA to explore the mysteries of the plant kingdom. - Eight basic experiments which can be used collectively or individually cover - Recombinant Cloning and Screening in E. coli; DNA Sequencing - Plant RNA Isolation and in Vitro Translations - Plant DNA Isolations and Genomic DNA Southern AnalysisChloroplast Isolation and Protein SynthesisPlant Tissue Culture and Agrobacterium Transformations - Experiments that have been student tested for three years - Blueprints for setting up gel rigs - Comprehensive course schedule outlining individual procedures to be finished in each lab segment - Course can be tailored to suit the needs of the individual instructor