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Methods in Plant Molecular Biology and Biotechnology

Author : Bernard R. Glick

Publisher : CRC Press

Page : 384 pages

File Size : 13,25 MB

Release : 2018-05-04

Category : Science

ISBN : 1351091395

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Book Description

Methods in Plant Molecular Biology and Biotechnology emphasizes a variety of well-tested methods in plant molecular biology and biotechnology. For each detailed and tested protocol presented, a brief overview of the methodology is provided. This overview considers why the protocol is used, what other comparable methods are available, and what limitations can be expected with the protocol. Other chapters in the book present overviews regarding how to approach particular problems and introduce unique methods - such as how to use computer methodology to study isolated genes. The book will be a practical reference for plant physiologists, plant molecular biologists, phytopathologists, and microbiologists.



Methods in Plant Biochemistry and Molecular Biology

Author : William V. Dashek

Publisher : CRC Press

Page : 477 pages

File Size : 12,92 MB

Release : 2018-05-04

Category : Science

ISBN : 1351091387

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Book Description

Modern plant science research currently integrates biochemistry and molecular biology. This book highlights recent trends in plant biotechnology and molecular genetics, serving as a working manual for scientists in academic, industrial, and federal laboratories. A wide variety of authors have contributed to this book, reflecting the thinking and expertise of active investigators who generate advances in technology. The authors were selected especially for their ability to create and/or implement novel research methods.



Plant Genetics and Molecular Biology

Author : Rajeev K. Varshney

Publisher : Springer

Page : 306 pages

File Size : 16,43 MB

Release : 2018-09-04

Category : Science

ISBN : 3319913131

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Book Description

This book reviews the latest advances in multiple fields of plant biotechnology and the opportunities that plant genetics, genomics and molecular biology have offered for agriculture improvement. Advanced technologies can dramatically enhance our capacity in understanding the molecular basis of traits and utilizing the available resources for accelerated development of high yielding, nutritious, input-use efficient and climate-smart crop varieties. In this book, readers will discover the significant advances in plant genetics, structural and functional genomics, trait and gene discovery, transcriptomics, proteomics, metabolomics, epigenomics, nanotechnology and analytical & decision support tools in breeding. This book appeals to researchers, academics and other stakeholders of global agriculture.



Plant Biotechnology and Genetics

Author : C. Neal Stewart, Jr.

Publisher : John Wiley & Sons

Page : 554 pages

File Size : 16,36 MB

Release : 2012-12-13

Category : Science

ISBN : 1118589483

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Book Description

Designed to inform and inspire the next generation of plant biotechnologists Plant Biotechnology and Genetics explores contemporary techniques and applications of plant biotechnology, illustrating the tremendous potential this technology has to change our world by improving the food supply. As an introductory text, its focus is on basic science and processes. It guides students from plant biology and genetics to breeding to principles and applications of plant biotechnology. Next, the text examines the critical issues of patents and intellectual property and then tackles the many controversies and consumer concerns over transgenic plants. The final chapter of the book provides an expert forecast of the future of plant biotechnology. Each chapter has been written by one or more leading practitioners in the field and then carefully edited to ensure thoroughness and consistency. The chapters are organized so that each one progressively builds upon the previous chapters. Questions set forth in each chapter help students deepen their understanding and facilitate classroom discussions. Inspirational autobiographical essays, written by pioneers and eminent scientists in the field today, are interspersed throughout the text. Authors explain how they became involved in the field and offer a personal perspective on their contributions and the future of the field. The text's accompanying CD-ROM offers full-color figures that can be used in classroom presentations with other teaching aids available online. This text is recommended for junior- and senior-level courses in plant biotechnology or plant genetics and for courses devoted to special topics at both the undergraduate and graduate levels. It is also an ideal reference for practitioners.



Advanced Methods in Molecular Biology and Biotechnology

Author : Khalid Z. Masoodi

Publisher : Academic Press

Page : 220 pages

File Size : 24,12 MB

Release : 2020-10-28

Category : Science

ISBN : 012824450X

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Book Description

Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. - Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology - Features clear, step-by-step instruction for applying the techniques covered - Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment



Molecular Biology Techniques

Author : Heather B. Miller

Publisher : Academic Press

Page : 227 pages

File Size : 45,9 MB

Release : 2011-10-18

Category : Science

ISBN : 0123855454

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Book Description

This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions



Practical Applications of Plant Molecular Biology

Author : Robert J. Henry

Publisher : Nelson Thornes

Page : 284 pages

File Size : 23,76 MB

Release : 1997

Category : Science

ISBN : 9780412732201

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Book Description

Practical Applications of Plant Molecular Biology is an important new title which covers the major techniques and how they are applied to a range of vitally important areas. Divided broadly into four sections, this book covers key subjects including the identification of plants and plant pathogens using molecular techniques, the estimation of genetic variation in plants, the use of molecular markers in plant improvement and the use of plant transformation techniques for the improvement of quality and the introduction of resistance. Also included is a comprehensive listing and description of the most frequently used techniques and a set of appendices covering useful topics of reference for the reader. All undergraduates studying plant sciences, molecular biology, biotechnology and agricultural sciences would benefit from having access to this title as would those studying for upper-level Masters courses concentrating on the disciplines covered. This book also provides an invaluable source of reference for professionals in agriculture, plant breeding, crop protection and improvement, biotechnology and molecular biology.



Current Technologies in Plant Molecular Breeding

Author : Hee-Jong Koh

Publisher : Springer

Page : 360 pages

File Size : 31,90 MB

Release : 2015-08-26

Category : Science

ISBN : 9401799962

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Book Description

Recent progress in biotechnology and genomics has expanded the plant breeders’ horizon providing a molecular platform on the traditional plant breeding, which is now known as ‘plant molecular breeding’. Although diverse technologies for molecular breeding have been developed and applied individually for plant genetic improvement, common use in routine breeding programs seems to be limited probably due to the complexity and incomplete understanding of the technologies. This book is intended to provide a guide for researchers or graduate students involved in plant molecular breeding by describing principles and application of recently developed technologies with actual case studies for practical use. The nine topics covered in this book include the basics on genetic analysis of agronomic traits, methods of detecting QTLs, the application of molecular markers, genomics-assisted breeding including epigenomic issues, and genome-wide association studies. Identification methods of mutagenized plants, actual case studies for the isolation and functional studies of genes, the basics of gene transfer in major crops and the procedures for commercialization of GM crops are also described. This book would be a valuable reference for plant molecular breeders and a cornerstone for the development of new technologies in plant molecular breeding for the future.



Plant Functional Genomics

Author : Erich Grotewold

Publisher : Springer Science & Business Media

Page : 443 pages

File Size : 46,97 MB

Release : 2008-02-03

Category : Science

ISBN : 1592594131

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Book Description

Functional genomics is a young discipline whose origin can be traced back to the late 1980s and early 1990s, when molecular tools became available to determine the cellular functions of genes. Today, functional genomics is p- ceived as the analysis, often large-scale, that bridges the structure and organi- tion of genomes and the assessment of gene function. The completion in 2000 of the genome sequence of Arabidopsis thaliana has created a number of new and exciting challenges in plant functional genomics. The immediate task for the plant biology community is to establish the functions of the approximately 25,000 genes present in this model plant. One major issue that will remain even after this formidable task is c- pleted is establishing to what degree our understanding of the genome of one model organism, such as the dicot Arabidopsis, provides insight into the or- nization and function of genes in other plants. The genome sequence of rice, completed in 2002 as a result of the synergistic interaction of the private and public sectors, promises to significantly enrich our knowledge of the general organization of plant genomes. However, the tools available to investigate gene function in rice are lagging behind those offered by other model plant systems. Approaches available to investigate gene function become even more limited for plants other than the model systems of Arabidopsis, rice, and maize.



Molecular Genetics, Genomics and Biotechnology of Crop Plants Breeding

Author : Søren K. Rasmussen

Publisher : MDPI

Page : 238 pages

File Size : 18,5 MB

Release : 2020-05-27

Category : Science

ISBN : 3039288776

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Book Description

This Special Issue on molecular genetics, genomics, and biotechnology in crop plant breeding seeks to encourage the use of the tools currently available. It features nine research papers that address quality traits, grain yield, and mutations by exploring cytoplasmic male sterility, the delicate control of flowering in rice, the removal of anti-nutritional factors, the use and development of new technologies for non-model species marker technology, site-directed mutagenesis and GMO regulation, genomics selection and genome-wide association studies, how to cope with abiotic stress, and an exploration of fruit trees adapted to harsh environments for breeding purposes. A further four papers review the genetics of pre-harvest spouting, readiness for climate-smart crop development, genomic selection in the breeding of cereal crops, and the large numbers of mutants in straw lignin biosynthesis and deposition.