Author : Fan-Gang Tseng
Publisher : MDPI
Page : 167 pages
File Size : 19,41 MB
Release : 2018-10-04
Category : Computers
ISBN : 3038421464
This book is a printed edition of the Special Issue "Micro/Nanofluidic Devices for Single Cell Analysis" that was published in Micromachines
Author : Tuhin Subhra Santra
Publisher : MDPI
Page : 254 pages
File Size : 10,43 MB
Release : 2021-06-02
Category : Science
ISBN : 3036506284
Cells are the most fundamental building block of all living organisms. The investigation of any type of disease mechanism and its progression still remains challenging due to cellular heterogeneity characteristics and physiological state of cells in a given population. The bulk measurement of millions of cells together can provide some general information on cells, but it cannot evolve the cellular heterogeneity and molecular dynamics in a certain cell population. Compared to this bulk or the average measurement of a large number of cells together, single-cell analysis can provide detailed information on each cell, which could assist in developing an understanding of the specific biological context of cells, such as tumor progression or issues around stem cells. Single-cell omics can provide valuable information about functional mutation and a copy number of variations of cells. Information from single-cell investigations can help to produce a better understanding of intracellular interactions and environmental responses of cellular organelles, which can be beneficial for therapeutics development and diagnostics purposes. This Special Issue is inviting articles related to single-cell analysis and its advantages, limitations, and future prospects regarding health benefits.
Author : Fan-Gang Tseng
Publisher : Springer
Page : 415 pages
File Size : 14,15 MB
Release : 2016-01-21
Category : Technology & Engineering
ISBN : 3662491184
This book provides an overview of single-cell isolation, separation, injection, lysis and dynamics analysis as well as a study of their heterogeneity using different miniaturized devices. As an important part of single-cell analysis, different techniques including electroporation, microinjection, optical trapping, optoporation, rapid electrokinetic patterning and optoelectronic tweezers are described in detail. It presents different fluidic systems (e.g. continuous micro/nano-fluidic devices, microfluidic cytometry) and their integration with sensor technology, optical and hydrodynamic stretchers etc., and demonstrates the applications of single-cell analysis in systems biology, proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics, biomedicine and drug delivery systems. It also discusses the future challenges for single-cell analysis, including the advantages and limitations. This book is enjoyable reading material while at the same time providing essential information to scientists in academia and professionals in industry working on different aspects of single-cell analysis. Dr. Fan-Gang Tseng is a Distinguished Professor of Engineering and System Science at the National Tsing Hua University, Taiwan. Dr. Tuhin Subhra Santra is a Research Associate at the California Nano Systems Institute, University of California at Los Angeles, USA.
Author : Fan-Gang Tseng
Publisher : MDPI
Page : 237 pages
File Size : 34,88 MB
Release : 2018-10-01
Category : Science
ISBN : 3038421936
This book is a printed edition of the Special Issue "Single Cell Analysis in Biotechnology and Systems Biology" that was published in IJMS
Author : Jin-Ming Lin
Publisher : Springer Nature
Page : 263 pages
File Size : 46,53 MB
Release : 2019-08-28
Category : Science
ISBN : 9813297298
This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor’s laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementary introduction and advanced guidebook, this book interests and inspires scholars and students who are currently studying or wish to study microfluidics-based cell analysis methods.
Author : Tuhin S. Santra
Publisher : CRC Press
Page : 548 pages
File Size : 17,38 MB
Release : 2020-11-01
Category : Medical
ISBN : 1000769135
The past two decades have seen rapid development of micro-/nanotechnologies with the integration of chemical engineering, biomedical engineering, chemistry, and life sciences to form bio-MEMS or lab-on-chip devices that help us perform cellular analysis in a complex micro-/nanoflluidic environment with minimum sample consumption and have potential biomedical applications. To date, few books have been published in this field, and researchers are unable to find specialized content. This book compiles cutting-edge research on cell manipulation, separation, and analysis using microfluidics and bio-MEMS devices. It illustrates the use of micro-robots for biomedical applications, vascularized microfluidic organs-on-a-chip and their applications, as well as DNA gene microarray biochips and their applications. In addition, it elaborates on neuronal cell activity in microfluidic compartments, microvasculature and microarray gene patterning, different physical methods for drug delivery and analysis, micro-/nanoparticle preparation and separation in a micro-/nanofluidic environment, and the potential biomedical applications of micro-/nanoparticles. This book can be used by academic researchers, especially those involved in biomicrofluidics and bio-MEMS, and undergraduate- and graduate-level students of bio-MEMS/bio-nanoelectromechanical systems (bio-NEMS), biomicrofluidics, biomicrofabricatios, micro-/nanofluidics, biophysics, single-cell analysis, bionanotechnology, drug delivery systems, and biomedical micro-/nanodevices. Readers can gain knowledge of different aspects of microfluidics and bio-MEMS devices; their design, fabrication, and integration; and biomedical applications. The book will also help biotechnology-based industries, where research and development is ongoing in cell-based analysis, diagnosis, and drug screening.
Author : Debmalya Barh
Publisher : Academic Press
Page : 492 pages
File Size : 42,54 MB
Release : 2019-06-06
Category : Medical
ISBN : 0128149205
Single-Cell Omics: Volume 1: Technological Advances and Applications provides the latest technological developments and applications of single-cell technologies in the field of biomedicine. In the current era of precision medicine, the single-cell omics technology is highly promising due to its potential in diagnosis, prognosis and therapeutics. Sections in the book cover single-cell omics research and applications, diverse technologies applied in the topic, such as pangenomics, metabolomics, and multi-omics of single cells, data analysis, and several applications of single-cell omics within the biomedical field, for example in cancer, metabolic and neuro diseases, immunology, pharmacogenomics, personalized medicine and reproductive health. This book is a valuable source for bioinformaticians, molecular diagnostic researchers, clinicians and members of the biomedical field who are interested in understanding more about single-cell omics and its potential for research and diagnosis. - Covers not only the technological aspects, but also the diverse applications of single cell omics in the biomedical field - Summarizes the latest progress in single cell omics and discusses potential future developments for research and diagnosis - Written by experts across the world, bringing different points-of-view and case studies to give a comprehensive overview on the topic
Author :
Publisher : Academic Press
Page : 328 pages
File Size : 29,58 MB
Release : 2022-01-13
Category : Science
ISBN : 0323989004
Micro/Nanofluidics and Lab-on-Chip Based Emerging Technologies for Biomedical and Translational Research Applications, Volume 185, Part A represents the collation of chapters written by eminent scientists worldwide. Chapters in this updated release include An introduction to microfluidics and their applications, Design and fabrication of Micro/Nanofluidics devices and systems, Detection and separation of proteins using Micro/Nanofluidics devices, Micro/Nanofluidics devices for DNA/RNA detection and separation, Paper based microfluidics a forecast towards the most affordable and rapid point-of-care devices, Paper based micro/Nanofluidics devices for biomedical applications, Advances of Microfluidics Devices and their Applications in Personalized Medicine, and much more. Additional chapters cover Microfluidics for single cell analysis, Fluorescence Based Miniaturized Microfluidic and Nanofluidic Systems for Biomedical Applications, Active Matter Dynamics in Confined Microfluidic Environments, Challenges and opportunities in micro/nanofluidics and lab-on-a-chip, and Paper-microfluidic signal-enhanced immunoassays. - Offers basic understanding of the state-of-the-art design and fabrication of microfluidics/ nanofluidics and lab-on-chip - Explains how to develop microfluidics/nanofluidics for biomedical application such as high throughout biological screening and separation - Discusses the applications, challenges and opportunities in biomedical and translational research applications of microfluidics/nanofluidics
Author : Jin-Ming Lin
Publisher : Springer
Page : 435 pages
File Size : 15,23 MB
Release : 2017-10-25
Category : Science
ISBN : 9811053944
This book presents a detailed overview of the design, formatting, application, and development of microfluidic chips in the context of cell biology research, enumerating each element involved in microfluidics-based cell analysis, discussing its history, status quo, and future prospects, It also offers an extensive review of the research completed in the past decade, including numerous color figures. The individual chapters are based on the respective authors' studies and experiences, providing tips from the frontline to help researchers overcome bottlenecks in their own work. It highlights a number of cutting-edge techniques, such as 3D cell culture, microfluidic droplet technique, and microfluidic chip-mass spectrometry interfaces, offering a first-hand impression of the latest trends in the field and suggesting new research directions. Serving as both an elementary introduction and advanced guidebook, the book interests and inspires scholars and students who are currently studying microfluidics-based cell analysis methods as well as those who wish to do so.
Author : Dimitrios Lamprou
Publisher : Springer Nature
Page : 499 pages
File Size : 37,91 MB
Release : 2023-04-27
Category : Science
ISBN : 303126908X
New materials and manufacturing techniques are evolving with the potential to address the challenges associated with the manufacture of medicinal products that will teach new tricks to old drugs. Nano- and microfabrication techniques include manufacturing methods such as additive manufacturing, lithography, micro-moulding, spray drying, and supercritical fluids among many others. The increasing resolution of new techniques allow researchers to produce objects with micrometric resolutions. This book follows a consecutive order, beginning with a background in the current field and limitations in the manufacturing of different pharmaceutical products, moving on the classification of each method by providing recent examples, and future prospective on a variety of traditional and new Nano and microfabrication techniques. A focus on the materials used to prepare these systems and their biocompatibility, including applied topics such as clinical applications and regulatory aspects also covered, offering the reader a holistic view of this rapidly growing field.
