Molecular Detection of Human Parasitic Pathogens


Book Description

Traditionally, laboratory identification of parasites has relied upon various phenotypic procedures that detect their morphological, biological, and immunological features. Because these procedures tend to be time-consuming and technically demanding, molecular methods based on nucleic acid amplification technologies have been increasingly utilized for rapid, sensitive, and specific characterization of parasites. The large number of original and modified molecular protocols that have been developed over the years creates a dilemma for those attempting to adopt the most appropriate protocol for streamlined identification and detection of human pathogenic organisms of interest. Part of a four-volume collection, Molecular Detection of Human Parasitic Pathogens provides a reliable and comprehensive resource on the molecular detection and identification of major human parasitic pathogens. This volume contains expert contributions from international scientists involved in human parasitic pathogen research and diagnosis. Following a similar format throughout, each chapter includes: A brief review on the classification, biology, epidemiology, clinical features, and diagnosis of an important pathogenic parasitic genus/group An outline of clinical sample collection and preparation procedures and a selection of representative stepwise molecular protocols A discussion on further research needs relating to improved diagnoses of major human parasitic pathogens This versatile reference on molecular detection and identification of major human parasitic pathogens is an indispensable tool for upcoming and experienced medical, veterinary, and industrial laboratory scientists engaged in parasite characterization. It is also suitable as a textbook for undergraduate and graduate students majoring in parasitology.




Advances in Automated Diagnosis of Intestinal Parasites of Animals and Humans


Book Description

Advances in Parasitology, Volume 116, the latest release in this ongoing series, includes medical studies of parasites of major influence, along with reviews of more traditional areas, such as zoology, taxonomy and life history. Chapters in this update include Landscape analysis of available diagnostic tests for STH: how far are we from the WHO TPPS? and Challenges and solutions for the diagnosis of animal and human Strongyloides stercoralis infection. Informs and updates on all the latest developments in the field of parasitology Includes medical studies of parasites of major influence Features reviews of more traditional areas, such as zoology, taxonomy and life history which will help shape current thinking and applications




Diagnostic Medical Parasitology


Book Description

This major reference -- now in its third edition -- updates and advances the field of diagnostic medical parasitology and reports on the dramatic changes that have occurred in this area. Newly recognized parasites, alternative techniques defined by new regulatory requirements, implementation of testing based on molecular techniques, and new information on parasitic infections are just a few of the changes covered in this new edition. As in previous editions, this book's approach is still to provide the user with clear, concise, well-organized procedures for use in the clinical laboratory. Along with these clinically relevant, practical, and cost-effective quality procedures, this text also provides a comprehensive discussion of two aspects of diagnostic medical parasitology, that is, the individual parasites and the relevant diagnostic methods designed to detect and identify the organisms present. These comprehensive discussions cover information related to life cycle, morphology, clinical disease, diagnosis, treatment, epidemiology, and prevention. Of particular importance in this third edition is the addition of Appendix 8, which contains information that includes late-breaking synopses of very recent publications. This appendix offers assistance in finding the most recent literature and information available. Updated and timely, this reference serves as a very comprehensive and practical guide full of necessary information for laboratorians, physicians, and health care professionals.




Procedure Manual for the Diagnosis of Intestinal Parasites


Book Description

Procedure Manual for the Diagnosis of Intestinal Parasites is the definitive resource for individuals involved in the collection, preparation, and examination of fecal specimens for microscopic diagnosis of intestinal parasitic infections. The book points out the stages of parasites possibly found in a fecal specimen, how to find them, and how to identify them. Specific details on how to effectively use the microscope for parasitic diagnosis are included. This information is missing from most texts and manuals of this kind. Photomicrographs and original drawings of the various stages and forms of parasites and eggs are used extensively throughout the text, with the photomicrographs printed to a standard scale for easy comparison. More than 400 illustrations in all are included. Biological keys for intestinal amoebae and eggs of various species of helminths are provided. This book is an essential reference for teachers of diagnostic parasitology and their students, physicians who order fecal examinations for intestinal parasites, nurses or health workers who handle or prepare the specimens for the laboratory, and technologists who receive, process, and examine the specimens.




Cyclospora and Cyclosporiasis


Book Description

Cyclospora cayetanensis is a microscopic parasite that can be transmitted via food or water sources and causes intestinal disease (Cyclosporiasis) in humans. Cyclospora and Cyclosporiasis: Epidemiology, Diagnosis, Detection, and Control reviews 22 species of Cyclospora and discusses C. cayetanensis infection in humans. This book offers comprehensive coverage that includes taxonomy and biology, molecular characteristics, and transmission of the parasite. It also provides researchers, clinicians, public health officials, and food safety officials with basic data for the epidemiology of the Cyclospora spp. or C. cayetanensis, as well as strategies to monitor large-scale outbreaks of Cyclospora spp. or C. cayetanensis. The book further covers clinical symptoms, diagnostic methods, and means of treatment and prevention of this disease and informs readers of the hazards of the parasite, common means of transmission, and self-protection measures. Provides the taxonomy of 22 species of Cyclospora (including two newly identified Cyclospora species) Covers the scenarios and characteristics of recent epidemics in the world, susceptible populations, and the risk factors in the transmission of the C. cayetanensis Summarizes recent developments in detection methods of Cyclospora spp. or C. cayetanensis in humans, animals, and the environment







Cryptosporidiosis and Microsporidiosis


Book Description

'... This volume provides most complete and balanced coverage of essential aspects of the pathogens as well as the diagnosis and clinical correlations of the disease they cause. It is set to become a valuable refrence for parasitologists, protozoologists, molecular biologists, clinical mecrobiologists, epidemiologists and specialists in infectious diseases.'




Blastocystis: Pathogen or Passenger?


Book Description

The stages of Blastocystis have been known for 101 years. However, many facts are still disputed, e.g. even the question whether it is a true pathogen or a commensal present in sometimes life-threatening diarrheas. The present book evaluates in chapters contributed by renowned researchers the latest findings on: •Landmarks in the discovery of Blastocystis •Epidemiology, transmission and zoonotic potential •Morphology of human and animal Blastocystis isolates •Clinical aspects of Blastocystis infections •Behavioral decision analysis: what makes us sick? •Blastocystis-host interactions •Molecular approaches on the systematical position •Genetic polymorphism •Blastocystis from a statistical point of view •Diarrheas due to different agents of disease •Zoonotic diseases in comparison As such, this book provides a broad range of information for people working in this field, for physicians and veterinarians who are confronted with clinical cases, teachers, students and technical staff members in the fields of microbiology, parasitology and diagnostic methods.




Sarcocystosis of Animals and Humans


Book Description

Sarcocystis is one of the most prevalent parasites of livestock and also infects many wild mammals, birds, and humans. Written by the authors who pioneered studies of Sarcocystosis of domestic animals, Sarcocystosis of Animals and Humans, Second Edition provides a current and comprehensive review of Sarcocystis and the infections it causes in anima