Molecular Techniques and Approaches in Developmental Biology
Author : Maarten J. Chrispeels
Publisher :
Page : 306 pages
File Size : 10,38 MB
Release : 1973
Category : Developmental biology
ISBN :
Author : Maarten J. Chrispeels
Publisher :
Page : 306 pages
File Size : 10,38 MB
Release : 1973
Category : Developmental biology
ISBN :
Author : Maarten J. Chrispeels
Publisher : Wiley-Interscience
Page : 324 pages
File Size : 41,90 MB
Release : 1973
Category : Medical
ISBN :
Author : Matt Guille
Publisher : Springer Science & Business Media
Page : 222 pages
File Size : 15,65 MB
Release : 2008-02-03
Category : Science
ISBN : 1592596789
The process whereby a single cell, the fertilized egg, develops into an adult has fascinated for centuries. Great progress in understanding that process, h- ever, has been made in the last two decades, when the techniques of molecular biology have become available to developmental biologists. By applying these techniques, the exact nature of many of the interactions responsible for forming the body pattern are now being revealed in detail. Such studies are a large, and it seems ever-expanding, part of most life-science groups. It is at newcomers to this field that this book is primarily aimed. A number of different plants and animals serve as common model org- isms for developmental studies. In Molecular Methods in Developmental Bi- ogy: Xenopus and Zebrafish, a range of the molecular methods applicable to two of these organisms are described, these are the South African clawed frog, Xenopus laevis, and the zebrafish, Brachydanio rerio. The embryos of both of these species develop rapidly and externally, making them particularly suited to investigations of early vertebrate development. However, both Xenopus and zebrafish have their own advantages and disadvantages. Xenopus have large, robust embryos that can be manipulated surgically with ease, but their pseudotetraploidy and long generation time make them unsuitable candidates for genetics. This disadvantage may soon be overcome by using the diploid Xenopus tropicalis, and early experiments are already underway. The transp- ent embryos of zebrafish render them well-suited for in situ hybridization and immunohistochemistry, and good for observing mutations in genetic screens.
Author :
Publisher : Academic Press
Page : 357 pages
File Size : 12,71 MB
Release : 1997-11-17
Category : Science
ISBN : 0080584594
This volume incorporates practical methods at the level of molecular, cellular, and whole organism biology in vertebrate and invertebrate models. It presents straightforward protocols written step-by-step for state-of-the-art techniques with the emphasis on single-cell resolution procedures. Provides straightforward, current protocols and critical appraisals Includes diverse analysis of cellular and molecular techniques Presents everything from whole-organ cultures to electrophysiological approaches Details a variety of methods for interfering with gene function in various species Offers multiple illusions of in situ hybridization, immunostaining, and apoptosis
Author : Rocky S. Tuan
Publisher : Springer Science & Business Media
Page : 628 pages
File Size : 15,24 MB
Release : 2000
Category : Science
ISBN :
Annotation This book combines expert reviews of the best contemporary methods for studying the disease process in ovarian cancer with detailed technical protocols for its analysis. The book focuses on the major problems still facing researchers on reproducible experimental methods to illuminate those problems and on gene therapeutic approaches to its treatment.
Author : Society for Developmental Biology. Symposium
Publisher : New York : A.R. Liss
Page : 188 pages
File Size : 40,7 MB
Release : 1986
Category : Science
ISBN : 9780845115046
Author : Jonathan M. W. Slack
Publisher : John Wiley & Sons
Page : 496 pages
File Size : 37,63 MB
Release : 2012-12-26
Category : Science
ISBN : 0470923512
Essential Developmental Biology is a comprehensive, richly illustrated introduction to all aspects of developmental biology. Written in a clear and accessible style, the third edition of this popular textbook has been expanded and updated In addition, an accompanying website provides instructional materials for both student and lecturer use, including animated developmental processes, a photo gallery of selected model organisms, and all artwork in downloadable format. With an emphasis throughout on the evidence underpinning the main conclusions, this book is an essential text for both introductory and more advanced courses in developmental biology. Shortlisted for the Society of Biology Book Awards 2013 in the Undergraduate Textbook category. Reviews of the Second Edition: "The second edition is a must have for anyone interested in development biology. New findings in hot fields such as stem cells, regeneration, and aging should make it attractive to a wide readership. Overall, the book is concise, well structured, and illustrated. I can highly recommend it." —Peter Gruss, Max Planck Society "I have always found Jonathan Slack's writing thoughtful, provocative, and engaging, and simply fun to read. This effort is no exception. Every student of developmental biology should experience his holistic yet analytical view of the subject." —Margaret Saha, College of William & Mary
Author : Rocky S. Tuan
Publisher : Springer Science & Business Media
Page : 537 pages
File Size : 29,52 MB
Release : 2008-02-05
Category : Science
ISBN : 1592590659
Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events—changes in form, structure, and function of the org- ism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and mole- lar biology, as well as evolutionary biology. No longer is the study of embryonic development merely “embryology.” In fact, development biology has produced - portant paradigms for both basic and clinical biomedical sciences alike. Although modern developmental biology has its roots in “experimental emb- ology” and the even more classical “chemical embryology,” the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the “cellular and molecular biology revolution.” The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.
Author : Gerald P. Schatten
Publisher : Elsevier
Page : 223 pages
File Size : 10,53 MB
Release : 2003-01-04
Category : Science
ISBN : 0080490875
Together with other volumes in this series, Volume 53 presents thoughtful and forward-looking articles on developmental biology and developmental medicine. The exceptional reviews in this volume of Current Topics in Developmental Biology will be valuable to both clinical and fundamental researchers, as well as students and other professionals who want an introduction to current topics in cellular and molecular approaches to developmental biology and clinical problems of aberrant development. This volume in particular will be essential reading for anyone interested in stem cells, signaling, medical implications of developmental determinants, hematopoeisis, axis specification, and molecular genetics of development. Includes a landmark review on Hedgehog genes and their gene products in invertebrate and vertebrate development Presents major issues and astonishing discoveries at the forefront of modern developmental biology and developmental medicine The longest-running forum for contemporary issues in developmental biology with over 30 years of coverage
Author : Paul T. Sharpe
Publisher : Springer Science & Business Media
Page : 752 pages
File Size : 49,44 MB
Release : 2008-02-02
Category : Science
ISBN : 1592592708
Most people have some interest in embryos; this probably results, in part, from their interest in understanding the biological origins of themselves and their offspring and, increasingly, concerns about how environmental change such as pollution might affect human development. Obviously, et- cal considerations preclude experimental studies of human embryos and, c- sequently, the developmental biologist has turned to other species to examine this process. Fortunately, the most significant conclusion to be drawn from the experimental embryology of the last two decades is the manner in which orthologous or closely related molecules are deployed to mediate similar - velopmental processes in both vertebrates and invertebrates. The molecular mechanisms regulating processes fundamental to most animals, such as axial patterning or axon guidance, are frequently conserved during evolution. (It is now widely believed that the differences between phyla and classes are the result of new genes, arising mostly by duplication and divergence of extant sequences, regulating the appearance of derived characters. ) Other vertebrates are obviously most likely to use the same devel- mental mechanisms as humans and, within the vertebrate subphylum, the - parent degree of conservation of developmental mechanism is considerable. It has long been recognized that particular vertebrate species offer either d- tinct advantages in investigating particular stages of development or are - pecially amenable to particular manipulations. No single animal can provide all the answers because not all types of experiments can be carried out on a single species.