Author : Christoph Kessler
Publisher : Springer Science & Business Media
Page : 1170 pages
File Size : 49,94 MB
Release : 2000-03-11
Category : Science
ISBN : 9783540646013
Highly sensitive systems which are widely used in molecular biological & biomedical laboratories, such as colorimetric, luminescence, fluorescence measuring using antibody-antigen binding or hybridisation, as well as PCR amplification are described in detail.
Author : Christoph Kessler
Publisher : Springer Science & Business Media
Page : 759 pages
File Size : 32,98 MB
Release : 2012-12-06
Category : Science
ISBN : 3642572065
New techniques and updated protocols for the detection and analysis of biomolecules - proteins, glycoproteins and nucleic acids. The second edition of this successful laboratory manual describes in detail the highly sensitive systems which are widely used in molecular biological and biomedical laboratories, such as colorimetric, luminescence, fluorescence measuring using antibody/antigen binding or hybridisation as well as PCR amplification. The clearly structured step-by-step protocols with practical hints and a troubleshooting guide are complemented by chapters on the theoretical background and the application of the techniques, enabling scientists to plan, design and conduct the appropriate procedures.
Author : Christoph Kessler
Publisher :
Page : 768 pages
File Size : 27,55 MB
Release : 2000-03-11
Category :
ISBN : 9783642572074
Author : Christoph Kessler
Publisher : Springer
Page : 449 pages
File Size : 41,48 MB
Release : 2012-12-06
Category : Science
ISBN : 3662001446
All the bioanalytical labeling and detection techniques in one source! This book gathers together all the important nonradioactive labeling techniques for nucleic acids, proteins, glycoproteins and glycolipids like Digoxigenin:Anti-Dioxigenin (DIG), Biotin, 5-Bromodeoxyuridine (BrdU), Sulfone, Immunogold, Silver Enhancement, and Synthetic Nucleic Acid Probe (SNAP) as well as the standard procedures for optical, chemical, biological, electrochemiluminescent and fluorescent detection. Additionally, applications for the use of non-isotopically labeled biomolecules are described. Specific protocols are given for hybridization analysis such as blot, colony/plaque and in-situ hybridization formats, quantitative formats, and also nonradioactivetechniques for nucleic acid sequencing and amplification. Each chapter contains a short introduction, a detailed description of the method with labprotocols, troubleshooting tips and references.
Author : Christoph Kessler
Publisher : Springer Verlag
Page : 436 pages
File Size : 47,26 MB
Release : 1992-01-01
Category : Science
ISBN : 9780387554822
Author : A. E. G. Cass
Publisher : Practical Approach
Page : 235 pages
File Size : 13,7 MB
Release : 1998
Category : Medical
ISBN : 0199636362
Biosensors are rising to the forefront of technology because they show that humans can harness the incredible functions of living molecules and cells and use them in valuable ways. Biomolecules and cells are critical components of biosensors. In order for these components to function in an artificial environment, they must be immobilized in a way that does not affect their interactions. This useful book provides a selection of the most current methods for immobilizing biomolecules and cells on a variety of surfaces so that they retain their functionality. The book also includes analytical techniques for measuring the functionality of immobilized biomolecules. All of the protocols have been tried and validated by the authors. The methods are easily repeatable, and the authors have carefully crafted the instructions so that they can be used without an extensive prior knowledge of biochemistry. Research in biosensors is carried out in a wide variety of fields including biochemistry, chemistry, engineering, laboratory medicine, environmental and defense research. This guide will be invaluable to researchers in all of these disciplines.
Author : Gary C. Howard
Publisher : McGraw-Hill/Appleton & Lange
Page : 342 pages
File Size : 33,10 MB
Release : 1993-01-01
Category : Science
ISBN : 9780838569467
Author : Stefan Lorkowski
Publisher : John Wiley & Sons
Page : 992 pages
File Size : 14,89 MB
Release : 2006-03-06
Category : Science
ISBN : 3527605339
This book combines the experience of 225 experts on 900 pages. Scientists worldwide are currently overwhelmed by the ever-increasing number and diversity of genome projects. This handbook is your guide through the jungle of new methods and techniques available to analyse gene expression - the first to provide such a broad view of the measurement of mRNA and protein expression in vitro, in situ and even in vivo. Despite this broad approach, detail is sufficient for you to grasp the principles behind each method. In each case, the authors weigh up the advantages and disadvantages, paying particular attention to the automated, high-throughput processing demanded by the biotech industry. Completely up to date, the book covers such ground-breaking methods such as DNA microarrays, serial analysis of gene expression, differential display, and identification of open reading frame expressed sequence tags. All the methods and necessary equipment are presented visually in more than 300 mainly colour illustrations to assist their step-by-step reproduction in your laboratory. Each chapter is rounded off with its own set of extensive references that provide access to detailed experimental protocols. In short, the bible of analysing gene expression.
Author :
Publisher : ScholarlyEditions
Page : 169 pages
File Size : 26,2 MB
Release : 2013-06-21
Category : Science
ISBN : 1481689959
Molecular Probes—Advances in Research and Application: 2013 Edition is a ScholarlyBrief™ that delivers timely, authoritative, comprehensive, and specialized information about ZZZAdditional Research in a concise format. The editors have built Molecular Probes—Advances in Research and Application: 2013 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about ZZZAdditional Research in this book to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Molecular Probes—Advances in Research and Application: 2013 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Author : Friedrich Lottspeich
Publisher : John Wiley & Sons
Page : 1137 pages
File Size : 41,72 MB
Release : 2018-03-08
Category : Science
ISBN : 3527694447
Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids. The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a bird's eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists. The following techniques are covered in detail: - Purification and determination of proteins - Measuring enzymatic activity - Microcalorimetry - Immunoassays, affinity chromatography and other immunological methods - Cross-linking, cleavage, and chemical modification of proteins - Light microscopy, electron microscopy and atomic force microscopy - Chromatographic and electrophoretic techniques - Protein sequence and composition analysis - Mass spectrometry methods - Measuring protein-protein interactions - Biosensors - NMR and EPR of biomolecules - Electron microscopy and X-ray structure analysis - Carbohydrate and lipid analysis - Analysis of posttranslational modifications - Isolation and determination of nucleic acids - DNA hybridization techniques - Polymerase chain reaction techniques - Protein sequence and composition analysis - DNA sequence and epigenetic modification analysis - Analysis of protein-nucleic acid interactions - Analysis of sequence data - Proteomics, metabolomics, peptidomics and toponomics - Chemical biology
