Test No. 222: Earthworm Reproduction Test (Eisenia fetida/Eisenia andrei)


Book Description

This Test Guideline is designed to be used for assessing the effects of chemicals in soil on the reproductive output (and other sub-lethal end points) of the earthworm species Eisenia fetida or Eisenia andrei. Adult worms are exposed to a range of ...




Test No. 222: Earthworm Reproduction Test (Eisenia Fetida/Eisenia Andrei)


Book Description

This Test Guideline is designed to be used for assessing the effects of chemicals in soil on the reproductive output (and other sub-lethal end points) of the earthworm species Eisenia fetida or Eisenia andrei. Adult worms are exposed to a range of concentrations of the test substance either mixed into the soil or applied to the soil surface. The range of test concentrations is selected to encompass those likely to cause both sub-lethal and lethal effects over a period of eight weeks. The limit test corresponds to one dose level of 1000 mg/kg. This study includes the observation of unusual behaviour and morphology, the counting and weighing of the adult worms after the four primary weeks, the number of juveniles hatched at the end of the second 4-week period. The reproductive output of the worms exposed to the test substance is compared to that of the control(s) in order to determine the no observed effect concentration (NOEC) and/or ECx by using a regression model to estimate the concentration that would cause a x % reduction in reproductive output. The test concentrations should bracket the ECx so that the ECx then comes from interpolation rather than extrapolation.




OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 207: Earthworm, Acute Toxicity Tests


Book Description

This Test Guideline includes two methods: a paper contact toxicity test and an artificial soil test. The recommended specie is Eisenia foetida (Michaelsen). The initial screening test (filter paper contact test) involves exposing earthworms to test ...







OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 218: Sediment-Water Chironomid Toxicity Using Spiked Sediment


Book Description

This Test Guideline is designed to assess the effects of prolonged exposure of chemicals to the sediment-dwelling larvae of the freshwater dipteran Chironomus sp. First instar chironomid larvae are exposed to at least five concentrations of the test ...




Test No. 220: Enchytraeid Reproduction Test


Book Description

This Test Guideline is designed to be used for assessing the effects of chemicals on the reproductive output of the enchytraeid worm, Enchytraeus albidus Henle 1873, in soil. Adult enchytraeid worms are exposed to a range of concentrations of the ...




OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 227: Terrestrial Plant Test: Vegetative Vigour Test


Book Description

This Test Guideline is designed to assess effects on vegetative vigour of terrestrial plants following above-ground exposure by general chemicals, biocides and crop protection products. The test can be conducted in order to determine the ...




OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 219: Sediment-Water Chironomid Toxicity Using Spiked Water


Book Description

This Test Guideline is designed to assess the effects of prolonged exposure of chemicals to the sediment-dwelling larvae of the freshwater dipteran Chironomus sp. First instar chironomid larvae are exposed to at least five concentrations of the test ...




OECD Guidelines for the Testing of Chemicals, Section 3 Test No. 317: Bioaccumulation in Terrestrial Oligochaetes


Book Description

This Test Guideline describes procedures designed to assess bioaccumulation of chemicals in soil oligochaetes. The parameters which characterise the bioaccumulation of a substance include the bioaccumulation factor (BAF), the uptake rate constant ...




OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 208: Terrestrial Plant Test: Seedling Emergence and Seedling Growth Test


Book Description

This Test Guideline is designed to assess effects on seedling emergence and early growth of higher plants following exposure to the test substance applied to the soil surface or into the soil. Seeds are placed in contact with soil treated with the ...